3.16 Chromatography

Question 1

How chromatography work?

Answer 1

separation depends on the affinity for mobile or stationary phase

Question 2

Thin Layer chromatography

Answer 2

Runs fast
Plates are robust
Plate is coated in silica gel- stationary phase
Solvent is mobile phase

Question 3

How do you calculate Rf value?

Answer 3

distant moved by solvent

Question 4

Column Chromatography

Answer 4

silica packed in tube- stationary phase
solvent, mobile phase is ran through tube

allows for silica to be used multiple times
allows for collection of individual constituents

Question 5

Gas Liquid Chromatography

Answer 5

stationary is a powder packed capillary tube
mobile is an inert gas

Different components leave tube at different times- retention time
accurate detectors used

Question 6

GCMS

Answer 6

Gas Liquid Chromatography with a Mass Spec attached