2.4 Production and destruction of RBCs Flashcards
Stimulus for production of RBCs?
Hypoxia
Source of EPO?
Peritubular interstitial cells (Kidneys)
A glycoprotein hormone and growth factor that is produce in the kidney
Erythropoietin
Functions of erythropoietin:
- Early release of _________ from the BM
- Preventing __________ of RBC progenitors
- Less time of RBC maturation in the BM
Reticulocytes
Apoptosis
Lab measurement for RBCs production:
What is the specimen used?
EDTA plasma
Specimen used indetermining RBCs production is commonly measured by?
Chemiluminescence
Reference interval for RBCs production in Chemiluminescence?
10 - 30 U/L
Major mechanism of Destruction of RBCs?
Extravascular hemolysis (80-90%)
Extravascular hemolysis mechanism is done through RES macrophage-medated
T or F
T
Destruction of RBCs is throug fragmentation of RBCs
Intravascular RBCs
A term where macrophage destroys OLD RBCs in Spleen
Splenic Culling
The process of Splenic culling:
Destruction of Old RBC releases?
Heme and Globin
The process of Splenic culling:
Once Heme is released, it is broken down into?
Iron and porphyrin
The process of Splenic culling:
Porphyrin is deoxidized by what enzyme which turns into biliverdin?
Heme oxygenase
The process of Splenic culling:
Biliverdin will convert in to ___________ that is calatyze by Biliverdin reductase
Unconjugated bilirubin
The process of Splenic culling:
B1 will be converted in to B2 in what organ?
Liver
The process of Splenic culling:
B2 will go to intestine and is reduce by bacteria into _____________?
Urobilinogen
The process of Splenic culling:
Urobilinogen:
Urine:__________
Stool:____________
Urobilinogen
Urobilin
Labs finding in excessive hemolysis for extravascular hemolysis:
_________ Serum Bilirubin (B1)
_________ Urine urobilinogen
All increase
Extravascular hemolysis: 80-90%
Intravascular hemolysis: 10-20%
T or F
T
Intravascular hemolysis:
How RBCs are fragmented is due to ?
Deposits (Fibrin, any deposits)
Intravascular hemolysis:
Fragmented RBCs are also known as?
Schistocytes
Intravascular hemolysis:
When hemoglobin is release to plasma, it will taken up by ___________ while heme will be taken up by?
Haptoglobin
Hemopexin
Intravascular hemolysis:
After heme and hemoglobin are taken up, it will go to the _________ for Hgb degradation
Liver
Labs finding in excessive hemolysis for intravascular hemolysis:
_________ Haptoglobin
_________ Hemopexin
All decrease
Labs finding in excessive hemolysis for intravascular hemolysis:
Decrease in haptoglobin is due to?
Hemoglobinuria
Labs finding in excessive hemolysis for intravascular hemolysis:
Excessive hemoglobinuria can lead to _________ where iron is lost in the urine
hemosiderinuria
Reference method for RBC survival studies?
Chromium (51 CR) Radioisotope labeling
