1.Lab Safety Flashcards

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1
Q

What is a hazard?

A

A hazard is anything that poses a potential threat to individuals or environment. It can include toxic or corrosive, chemicals, heat or flammable substances, pathogenic organisms and mechanical equipment.

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2
Q

What is a risk?

A

Risk is the likelihood of harm arising from exposure to a hazard. a risk assessment, reduces the risk of Hazard.

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3
Q

What is a risk assessment?

A

A risk assessment involves identifying control measures to minimise the risk control measures include using appropriate handling techniques, protective clothing, equipment and aseptic technique.

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4
Q

What is linear dilution?

A

What are the dilution series difference by equal intervals for example, 0.1 0.2 0.3 et cetera

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5
Q

What is a log or serial dilution?

A

It differs by a constant proportion

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6
Q

What are the different equipment able to measure liquids?

A

Measuring cylinders, pipettes, buttered auto pipettes and syringes

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7
Q

What are colorimeter?

A

Colorimeters are devices that can be used to quantify concentrations.

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8
Q

What do colorimeters measure?

A

The absorbance of specific wavelengths of light by solution.

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9
Q

What can absorbance be used to determine?

A

The concentration of a coloured pigment

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10
Q

What can percentage transmission be used to determine?

A

Turbidity such as cells in suspension

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11
Q

What is a standard curve?

A

The standard curve is one method used to determine an unknown concentration of a solution.

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12
Q

What is pH?

A

pH is a measure of the acidity or basicity of a solution. It is a logarithmic measure of hydrogen ion concentration in a solution.

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13
Q

How can pH be measured?

A

By indicator or electronic meter

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14
Q

What are the advantages of using an indicator?

A

Maybe good for field usage or measuring small volumes are dealing with noxious samples

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15
Q

What are the disadvantages of using an indicator?

A

Less precise and each indicator is only good for a small pH range

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16
Q

What is the advantage of using electronic metres?

A

Accurate and can use data logging on computer

17
Q

What is the disadvantage of using an electronic meter?

A

Need for frequent calibration

18
Q

What is a pH buffer used for?

A

To control pH.

19
Q

How can substance be separated using their physical properties?

A

Solubility, size/density, shape and charge

20
Q

What is centrifugation?

A

Centrifugation is when a liquid is spun at very high speed for a period of time.

21
Q

What is the use of a centrifuge?

A

The use of a centrifuge separate substances in the liquid by differing density.

22
Q

Where does the largest and densest material separate and settle?

A

In the pellet at the bottom of the tube

23
Q

What is the remaining liquid is known as above the pellet

A

Supernatant