1.4 immobilized enzymes Flashcards

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1
Q

what are immobilised enzymes

A

immobilized enzymes are fixed bound or trapped on an inert matrix(or membrane)
∴ reducing frequency of collisions (substrate is the only moving molecule)

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2
Q

where are immobilized enzymes used

A

(industrial)
- food
- pharmaceutical
- agrochemical

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3
Q

what is an example of an immobilized enzyme

A

alginate beads

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4
Q

advantages of immobalized enzymes:

A
  • can be reused
  • only a small enzyme quantity needed
  • greater stability ∴ denature at higher temps
  • no contamination
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5
Q

disadvantages of immobilised enzymes

A
  • cost a lot
  • enzymes can become less active
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6
Q

how does a biosensor that detects urea function

A
  • urea molecules diffuse across semi-permeable membrane ∴ enzyme-substrate complex (immobilized urease)
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7
Q

urea + urease =

A

ammonia

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8
Q

what is a biosensor

A

analyse the concentration of substances in a solution

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9
Q
A
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