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Micro 🦠 > عملي مايكرو > Flashcards

عملي مايكرو Flashcards

1
Q
A

( Flame source )

Dry heat is to kill microorganisms

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2
Q
A

( Safety box )

Contaminated sharp to be incinerated

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3
Q
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( Disposable bag )

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4
Q
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Sodium hypochlorite ( CLORX )

Use : Decontamination of liquid waste , surface and some medical equipment.

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5
Q
A

Iodophores

Use : 1- hand hygiene
2- it can applied on mucous membranes

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6
Q
A

Gram negative rod , Gram stain

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7
Q
A

Filamentous fungi

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8
Q
A

Chocolate agar “heated blood agar”

Can be cultured with : Haemophilus influenza Neisseria

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9
Q
A

Blood agar

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10
Q
A

MacConkeys agar

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11
Q
A

Thiosulfate - citrate - bile salts - sucrose agar ( TCBS )

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12
Q
A

Robertson CMM ( Enriched )

For growth of anaerobic organism

Eg. Clostridium

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13
Q
A

( Metalic loop )

Method of sterilization is by Red heat

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14
Q

Identify :
Stain :
Shape :
Example of possible organisms :

A 
Identify : 
Spirochetes 
Stain :
Fontana stain 
Shape : 
Twisted spirally ( corkscrew ) 
Example of possible organisms : 
 Treponema Pallidum
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15
Q

Identify ?

Stain ?

Arrangement ?

Example ?

A

Identify : Staphylococci in Culture
Stain : Gram +ve
Arrangement : Grape-like irregular clusters
Example : S.aureus , S.epidermidis

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16
Q

Identify ?

Stain ?

Arrangement ?

Example ?

A

Identify : Streptococci, in Culture
Stain : Gram +ve
Arrangement : Chain
Example : S. pyogens , S. agalactiae & S. viridans

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17
Q

Identify ?
Stain ?
Arrangement ?

A

Identify : Corynebacterium Diphtheria
Stain : Gram + ve
Arrangement : V shape giving Chinese letter appearance

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18
Q 
Identify ? 
Stain ? 
shape ? 
Arrangement ? 
Example?
A 
Identify : Bacillus group 
Stain : Gram +ve ( gram stain ) 
shape : Rods 
Arrangement : Long Chains 
Example : B. anthracis & B. cereus
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19
Q
A

Identify : Bacillus group
Stain : Gram +ve ( spore stain )
shape : Rods
Arrangement : Long Chains

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20
Q
A

Clostridium tetani

G +ve bacilli arranged in Drum stick appearance with unstained terminal spores

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21
Q
A 
Identify : Gram Negative Diplococci
Stain : Gram –ve 
Shape : Diplococci 
Arrangement : Pairs 
Example : Neisseria meningitides & Neisseria gonorrhea
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22
Q
A

Identify: Gram –ve bacilli
Stain : Gram –ve
Shape : straight bacilli
Example : E. coli

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23
Q
A

Identify : Gram –ve bacilli
Stain : Gram –ve
Shape : curved bacilli
Example : Vibrio cholera , Helicobacter, Cambylobacter

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24
Q
A

Staphylococcus aureus in pus
Gram +ve cocci arranged in clusters ( Grape like
Staphylococci aureus
+ Present in pus

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25
Q
A

Staphylococcus aureus in pus
Gram +ve cocci arranged in clusters ( Grape like
Staphylococci aureus
+ Present in pus

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26
Q
A

Staphylococcus aureus in pus
Gram +ve cocci arranged in clusters ( Grape like
Staphylococci aureus
+ Present in pus

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27
Q
A

Staphylococcus aureus in pus
Gram +ve cocci arranged in clusters ( Grape like
Staphylococci aureus
+ Present in pus

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28
Q
A

Streptococcus pyogenes in pus

Gram +ve cocci arranged in chains + present in pus

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29
Q
A

Streptococcus pyogenes in pus

Gram +ve cocci arranged in chains + present in pus

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30
Q
A

Streptococcus pyogenes in pus

Gram +ve cocci arranged in chains + present in pus

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31
Q
A

(/Meningococci / Gonococci )

Gram -ve diplococci arranged in pairs with kidney shape appearance + present in pus

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32
Q
A

Bacillus anthracis in tissue

G +ve bacilli arranged in long chain + present in pus

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33
Q
A

Identify: Acid Fast Bacilli
Stain : Ziehl Neelsen
Example : Mycobacterium Tuberculosis in sputum

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34
Q
A

Mycobacterium tuberculosis in sputum stained by Ziehl Neelsen

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35
Q
A

Mycobacterium leprae

Stain : Modified Ziehl Neelsen

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36
Q
A

Id entify : Spirochetes
Stain : Fontana stain
Shape : Twisted spirally ( corkscrew )
Example : Treponema Pallidum

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37
Q
A

Gram +ve budding yeast Example ; Candida albicans

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38
Q

Specific test to differentiate between Candida albicans from other albicans?

A

Germ tube test

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39
Q
A

Gram +ve ( Candida albicans )

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40
Q
A

Cryptococcus neoformans

Black background by India ink stain

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41
Q
A

Cryptococcus neoformans

black background by Direct Immunofluoresce

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42
Q
A

CPE of CMV (Intranuclear inclusion bodies with Owl’s eye appearance)

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43
Q
A

CPE of CMV (Intranuclear inclusion bodies with Owl’s eye appearance)

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44
Q
A

Heavy duty gloves

Safe for holding and cleaning of sharp instruments ( surgical scissors , and instruments )

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45
Q
A

Goggles

Should be used to avoid splashes of infectious material to the eye

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46
Q
A

( Autoclave )

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47
Q
A

( swab stick )

For collection of specimen

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48
Q
A

Nutrient agar (Basic culture media)

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49
Q
A

( Endopigment )

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50
Q
A

Exopigment

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51
Q
A

( Macconkeys agar media )
Showing lactose fermenting colonies ( LFC )
Example : E.coli

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52
Q
A

Lactose fermenter on MacConkey agar

Example : klebsiella

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53
Q
A

Blood agar
Beta hemolytic
Example : S.aureus

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54
Q
A

Blood agar

Alpha hemolysis

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55
Q
A

Filamentous fungi , Aspergillus in culture, lactophenol cotton blue stain .

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56
Q
A

Heavy duty gloves

Safe for holding and cleaning of sharp instruments ( surgical scissors , and instruments )

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57
Q
A

Goggles

Should be used to avoid splashes of infectious material to the eye

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58
Q
A

Surgical mask

To prevent transmission of droplets and protect against infection.

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59
Q
A

Lowenstein – Jensen Medium

Uses: For isolation of mycobacterium tuberculosis

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60
Q
A

Loffler’s Serum Media
Type: Enriched
Uses: isola:on of Corynebacterium diphtheria

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61
Q
A

Robertson’s Cooked Meat Media
Type: Enrichment
Uses: For growth of anaerobic organism
e.g. Clostridium

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62
Q

البول الاسود، جوا

A

Bile Aesculin Agar showing ( Black discoloration )

Ex. Enterococcus

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63
Q

لاهوائي و غاز و ظهر الجار

A

Anaerobic Gas Pack Jar
Uses: - For isolation of anaerobic organisms
e.g. all Clostridium sp.
For isolation of microaerophilic microorganisms
e.g. Campylobacter & Helicobacter

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64
Q

انا بروفيسوره هندية

A

AP I (Analytical Profile Index) test

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65
Q

الازرق كوب عليه مويه KP

الاخضر يعني إفا E

A

Citrate utilization test
Positive (Blue)
Ex: 1- Klebsiella
2- pseudomonas aeroginosa

Negative (Green)
Ex. E.coli

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66
Q

المجنون يحب اللون الوردي اكثر من مره و يحب الجلوكوز و المالتوز

A

Sugar utilization test for Neisseria

Meningococci: Showing fermentation of glucose and maltose

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67
Q

جنى تحب اللون الاصفر و الوردي و تحب الجلوكوز بس

A

Gonococci: Showing fermentation of glucose ONLY

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68
Q
A

Brucella slide test , slide agglutination test

For serotyping of bacteria

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69
Q
A

Swarming of proteus on nutrient agar

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70
Q

ثايو السافل ستر البول المالح السكروزي

A

Thiosulphate citrate bile salt sucrose agar (TCBS)
Growth of non sucrose fermenting colonies
Use in :
1- vibrio genus
2- vibro cholera

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71
Q
A

MacConkey agar
Growth of lactose fermenter ( LFC )
Use in :
E. Coli

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72
Q
A

MacConkey agar
Growth of lactose fermenter ( LFC )
Use in :
E. Coli

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73
Q
A

MacConkey agar
Growth of lactose fermenter ( LFC )
Use in :
E. Coli

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74
Q
A

MacConkey agar Non lactose fermenter

Proteus

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75
Q
A

Custine lactose Electrolyte Deficient agar ( CLED )
Growth of lactose fermenter
Semiquantitave urine culture
E. Coli

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76
Q
A

Custine lactose Electrolyte Deficient agar ( CLED )
Growth of lactose fermenter
Semiquantitave urine culture
E. Coli

77
Q
A

Exopigment of pseudomonas on nutrient agar

78
Q
A

Exopigment of pseudomonas on nutrient agar

79
Q
A

Exopigment of pseudomonas on Mac Conkey agar

80
Q
A 
Staphylococcus aureus in pus 
Gram +ve cocci arranged in clusters ( Grape like
Staphylococci aureus
\+ Present in pus
Virulence factor: 
1- Protein A
2- Clumping factors (bound coagulase)
Disease : Impetigo
81
Q
A 
Staphylococcus aureus in pus 
Gram +ve cocci arranged in clusters ( Grape like
Staphylococci aureus
\+ Present in pus
Virulence factor: 
1- Protein A
2- Clumping factors (bound coagulase)
Disease : Impetigo
82
Q
A 
Staphylococcus aureus in pus 
Gram +ve cocci arranged in clusters ( Grape like
Staphylococci aureus
\+ Present in pus
Virulence factor: 
1- Protein A
2- Clumping factors (bound coagulase)
Disease : Impetigo
83
Q
A 
Staphylococcus aureus in pus 
Gram +ve cocci arranged in clusters ( Grape like
Staphylococci aureus
\+ Present in pus
Virulence factor: 
1- Protein A
2- Clumping factors (bound coagulase)
Disease : Impetigo
84
Q
A 
Streptococcus pyogenes in pus 
Gram +ve cocci arranged in chains + present in pus
Virulence factor: 
1- M protein 
2- IgG degrading enzyme
Disease : 
1- acute pharyngitis
2-impetigo
Treatment: penicillin G
85
Q
A 
Streptococcus pyogenes in pus 
Gram +ve cocci arranged in chains + present in pus
Virulence factor: 
1- M protein 
2- IgG degrading enzyme
Disease : 
1- acute pharyngitis
2-impetigo
Treatment: penicillin G
86
Q
A

Pneumococci
Gram +ve cocci arranged in chains or diplos surrounded by unstained area + present in pus
Virulence factors:
1- Polysaccharide capsule 2- IgA1 protease

Diseases:
1- Otitis media
2- Lobar pneumonia

87
Q
A

Pneumococci
Gram +ve cocci arranged in chains or diplos surrounded by unstained area + present in pus
Virulence factors:
1- Polysaccharide capsule 2- IgA1 protease

Diseases:
1- Otitis media
2- Lobar pneumonia

88
Q
A

Pneumococci
Gram +ve cocci arranged in chains or diplos surrounded by unstained area + present in pus
Virulence factors:
1- Polysaccharide capsule 2- IgA1 protease

Diseases:
1- Otitis media
2- Lobar pneumonia

89
Q
A

Pneumococci
Gram +ve cocci arranged in chains or diplos surrounded by unstained area + present in pus
Virulence factors:
1- Polysaccharide capsule 2- IgA1 protease

Diseases:
1- Otitis media
2- Lobar pneumonia

90
Q
A

Meningococci / Gonococci

Gram -ve diplococci arranged in pairs with kidney shape appearance + present in pus

91
Q
A

Bacillus anthracis in tissue
G +ve bacilli arranged in long chain + present in pus

Virulence:
1-polypeptide Capsule
2- Anthrax toxin

92
Q
A

Identify : Acid fast bacilli
Stain : Ziehl Neelsen
Example : Mycobacterium Tuberculosis in sputum

93
Q
A

Mycobacterium leprae arranged in masses or groups mostly intracellular stained by Modified Ziehl Neelsen

94
Q
A

Candida albicans

Germ tube test +ve

95
Q
A

Candida albicans

Germ tube test -ve

96
Q
A

Candida albicans

Germ tube test +ve

97
Q
A

Cryptococcus neoformans, Capsulated budding yeast (India ink stain)

98
Q
A

Cryptococcus neoformans, Capsulated budding yeast (India ink stain)

99
Q
A

Cryptococcus neoformans, Capsulated budding yeast (India ink stain)

100
Q
A

Cryptococcus neoformans Direct Immunofluoresce

101
Q
A

Aspergillus flavus (lactophenol cotton blue)

102
Q
A

CPE of Herpes simplex (Rounding and ballooning of cells grape like)

103
Q
A

CPE of Herpes simplex (Rounding and ballooning of cells grape like)

104
Q
A

CPE of RSV (Fusion of cells with syncytia formation) Syncytial formation caused by RSV in cell culture )

Disease :bronchiolitis and pneumonia in infants

105
Q
A

Nutrient agar (Basic culture media)

106
Q

Spicemen: ( CSF ) , blood , Nasopharyngeal swap in carrier’s ?

A 
Chocolate agar (heated blood agar) (Enriched) 
Neisseria meningitis
107
Q

Spicemen : urethral discharge, cervical samples , conjunctival swap ?

A

modified Thayer Martin medium

Neisseria gonorrhoea

108
Q

Spicemen : sputum , CSF , pus ?

A 
Chocolate agar (heated blood agar) (Enriched) 
Haemophilus influenza
109
Q
A

Thiosulph ate citrate bile salt sucrose agar (TCBS)
Uses: Isolation of vibrio
Can be cultured with: Vibrio cholerae showing yellow sucrose fermenting colonies

110
Q
A

Thiosulphate Citrate Bile Salt Sucrose Agar (TCBS)
Cultured with: vibrio parahemolyticus
Showing: colorless non lactose fermenting colonies

111
Q
A

Cysteine Lactose Electrolyte deficient media (CLED)

Used for urine culture to inhibit swarming of proteus

112
Q
A

Cysteine Lactose Electrolyte deficient media (CLED)

Non selective differential/indicator media for isolation of urinary pathogens

113
Q
A

Loffler’s serum media
Type: Enriched
Uses: Rapid isola:on of Corynebacterium diphtheria

114
Q
A

Lowenstein – Jensen Media
Type: Enriched, Selective & Indicator (differential)
Uses: For isolation of mycobacterium tuberculosis

115
Q
A

Robertson’s Cooked Meat Media

Uses: For growth of anaerobic organism e.g. Clostridium

116
Q
A

Nutrient Agar
Endopigment
Staphylococcus aureus give golden yellow endopigment on nutrient agar

117
Q
A

Nutrient agar
Exopigment
Pepseudomonas aeruginosa give greenish exopigment on nutrient agar

118
Q
A

( Cell culture for virus culture )(For virus isolation and identification )

-Viral culturing: e.g. Polymyelitis & Herbs viruses

119
Q
A

Anaerobic Gas Pack Jar
Us es: - For isolation of anaerobic organisms e.g. all Clostridium sp.
- For isolation of microaerophilic microorganisms e.g. Campylobacter & Helicobacter

120
Q
A

Novobiocin sensitivity

To Differentiates S. epidermidis from S. saprophyticus

121
Q

حساسة ما تحب احد يجي جنبها

A

Novobiocin sensitivity
S. epidermidis
Skin
Sensitive

122
Q

ليست حساسة و الكل جنبها

A

( Novobiocin sensitivity )

S. saprophyticus
URINE
Resistance

123
Q
A

Novobiocin sensitivity
S. epidermidis
Skin
Sensitive

124
Q
A

( Novobiocin sensitivity )

S. saprophyticus
URINE
Resistance

125
Q

مكتوب على الدائرة B او A كلهم نفس الشيء

A

Bacitracin sensitivity

Group A streptococci sensitive ( streptococcus pyogenes)

126
Q

مكتوب على الدائرة B او A كلهم نفس الشيء

A

Bacitracin resistant

Streptococcus group A , resistant ( streptococcus agalactiae )

127
Q
A

Bacitracin sensitivity

Group A streptococci sensitive ( streptococcus pyogenes)

128
Q
A

Bacitracin sensitivity

Group A streptococci sensitive ( streptococcus pyogenes)

129
Q
A

Bacitracin sensitivity

Group A streptococci sensitive ( streptococcus pyogenes)

130
Q

O او P نفس الشيء

A

Optochin sensitivity (Pneumococci sensitive)

131
Q

O او P

A

Optochin sensitivity (Pneumococci sensitive)

132
Q

O او P

A

Optochin sensitivity (Pneumococci sensitive)

133
Q
A 
Optochin sensitivity 
optochin resistance ( Viridans streptococci )
134
Q

است

A

Antibiotic Sensitivity Testing (Disc diffusion method)
Used fo test antibiotics sensitivity by Disc
diffusion method

135
Q
A

ELEK’s test (Ag/Ab precipitation test, double diffusion of Ag/Ab in gel) Toxigenic strains of C.diphtheria

136
Q
A

ELEK’s test

Toxigenic C.diphtheria

137
Q
A

ELEK’s test

Non - toxigenic C.diphtheria

138
Q
A

ELEK’s test

139
Q
A

Nagler’s reaction (toxin neutralization) Clostridium perfringenes
Antitoxin

140
Q
A

Nagler’s reaction (toxin neutralization) Clostridium perfringenes
No Antitoxin

141
Q
A

Nagler’s reaction

Used for toxin neutralization of clostridium perfringenes

142
Q
A

Pseudomonas aeruginosa on nutrient agar

showing greenish exopigment

143
Q
A

Proteus on nutrient agar showing swarming growth

144
Q
A

Mannitol salt agar

145
Q
A

Mannitol salt agar

Staphylococcus aureus

146
Q
A

Mannitol salt agar

Staphylococcus epidermidis

147
Q
A

Mannitol salt agar

Staphylococcus aureus

148
Q
A

Mannitol salt agar

149
Q
A

Mannitol salt agar

Staphylococcus aureus

150
Q

الثقيلة

A

Lactose fermenting colonies in MacConkeys agar and mucoid is Klebsiella

151
Q

الثقيلة

A

mucoid and Lactose fermenting colonies in MacConkeys agar is klebsiella

152
Q

الخطوط الخفيفة

A

MacConkeys agar Lactose fermenting colonies , E. coli

153
Q
A

MacConkeys agar Lactose fermenting colonies , E. coli

154
Q
A

Non Lactose fermenting colonies (NLFC)

•Salmonella •Shigella •Proteus

155
Q
A

Non hemolytic colonies

156
Q
A

Blood agar , Non hemolytic colonies

157
Q
A

Blood agar , Non hemolytic colonies

158
Q
A

Blood Agar à Swarming Growth

Cultured with: Proteus

159
Q
A

Blood tellurite (Black colonies of Corynaebacterium diphtheria)

160
Q
A

Blood tellurite (Black colonies of Corynaebacterium diphtheria)

161
Q
A

Blood tellurite (Black colonies of Corynaebacterium diphtheria)

162
Q
A

Alpha hemolysis in Chocolate agar (pneumococci/Strept virridans)

163
Q
A

Sabouraud’s dextrose agar Filamentous fungi

164
Q

Bcb

A

Blood cultu re bottles
Type : Enrichment

Used for culture of blood Examples/ culture of circulating organisms in blood In case of Bacteremia,
septicaemia, typhoid fever

165
Q
A

Tube agglutination test (standard agglutination test; SAT) e.g diagnosis of brucellosis

166
Q
A

Tube agglutination test (standard agglutination test; SAT) e.g Prozone phenomenon

167
Q
A

Tube agglutination test (standard agglutination test; SAT) e.g Prozone phenomenon

168
Q

انتي الانبوبه الحمراءinti الدويري O

A

Antistreptolysin O test (ASOT) for diagnosis of rheumatic fever (+ve tube showing no hemolysis)

169
Q

الدوائر الصفراء يعني ترتر صغير MT حق اليسا

A

Microtiter plate (ELISA plate) (Detection of Ag or Ab)

170
Q
A

Coagulase production test , positive, staphylococcus aureus

171
Q
A

Coagulase production test , negative, staphylococcus epidermidis

172
Q
A

Urease production test Positive , proteus

173
Q
A

Urease production test , negative

174
Q

بيه

A

Bile esculin hydrolysis test (Positive  Enterococci)

175
Q
A

Agglutination test Blood grouping

176
Q
A

Slide agglutination test serogrouping/serotyping of bacteria

177
Q
A

Catalase test , catalase positive, staphylococcus aureus

178
Q
A

Catalase test , catalase positive , staphylococci

179
Q
A

Catalase test , catalase negative , streptococci

180
Q
A

Catalase test , catalase positive , staphylococci

181
Q
A

Catalase test , catalase negative , streptococci

182
Q
A

Oxidase test

183
Q
A

Oxidase test , Oxidase positive , Neisseria , pseudomonas

184
Q
A

Oxidase test , oxidase negative , streptococci

185
Q
A

Oxidase test

186
Q
A

Oxidase test

187
Q

است

A

Antibiotic sensitivity Testing
Used for test antibiotics sensitivity by Disc
diffusion method

188
Q
A

Identify: Antiseptics
Uses : used for hand hygiene, preparation of patient skin before invasive procedures
Example : alcohols, chlorhexidine and iodophores (Betadine)

Micro 🦠 (41 decks)

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