Weirauch Flashcards
Flashcards for Weirauch Lecture
___________ regulation is the control of gene production.
Gene
Transcription Factors (TFs) are ___________ that can turn on or off the synthesis of genes in response to specific cues.
proteins
The two broad categories of gene regulation are ___________ regulation and ___________ regulation.
transcriptional, post-transcriptional
The human genome has approximately ___________ TFs.
1639
TFs interact with the genome by binding to ___________.
short sequences
Each Transcription Factor recognizes a specific ___________ in the genome.
DNA motif
TF regulatory elements are usually located near the ___________ that they control.
genes
Humans have roughly ___________ genes.
20,000
___________ is the process by which DNA is transcribed into RNA.
Transcription
___________ footprinting is bad for sensitivity.
Phylogenetic
When a library of PWMs is scanned to see if any are statistically over-represented in a set, ___________ motifs are being used.
known
When searching for common patterns in a set, ___________ motif analysis is being employed.
de novo
___________ data is one type of functional data that can be leveraged to understand TF binding and function.
ENCODE
___________ of TFs and co-factors is one factor that influences TF binding and function.
Relative concentrations
___________ accessibility impacts TF binding and function.
Chromatin
The ___________ status of DNA can influence TF binding and function.
methylation
Both local and ___________ topology of DNA plays a role in TF binding and function.
long-range
___________ are stretches of DNA that encode blueprints for making proteins.
Genes
Every cell in an organism has the same ___________.
genomic DNA
T/F: Genes are not all used at all times.
True
The genome is like a ___________, while transcriptional regulation is like following a ___________ to make a cake.
recipe book, recipe
A ___________ experiment uses a library of random oligos and multiple rounds of selection/amplification to determine which TF can bind.
SELEX
A ___________ is a representation of the binding preferences of a transcription factor.
position frequency matrix (PFM)
___________ can affect TF binding specificity.
cofactor interactions
The prevalence of how co-factor interactions can affect TF binding specificity is largely ___________.
unknown
___________ positioning, which dictates chromatin accessibility, is a factor that can influence TF binding and function.
Nucleosome
Humans are composed of about ___________ cells.
100 trillion
Human and ___________ DNA is greater than 99% identical.
Chimp
