week week 2 BASIC HEMATOLOGICAL METHODS OF EXAMINATION QUALITY ASSURANCE PROGRAM COLLECTION OF BLOOD ANTICOAGULANT Flashcards
COMPLETE BLOOD COUNT
- Basic screening test and is one of the most frequently ordered laboratory procedure.
most commonly requested lab test
complete blood count
- Gives valuable diagnostic information about the hematologic and other body system , prognosis response to treatment and recovery
COMPLETE BLOOD COUNT
COMPLETE BLOOD COUNT test the following:
Hgb, Hct, RBC and WBC count, Differential Count
rbc parameters
rbc count
hgb
hct
mcv
mchc
rdw - red distribution width
retic
platelets parameters
plt count and mpv (mean platelet volume)
if your mean platelet volume has a reference range of
8-10 femtoliters
if the mpv is in 8-10 your platelet has a normal size of
2-4 um
wbc parameters
wbc count
the main component of erythrocytes, serves as the vehicle for the transportation of oxygen and carbon dioxide.
hemoglobin
- Measurement of the concentration of Hgb in the blood
hemoglobin determination
reagent used for cyanmethemoglobin method
Drabkin’s reagent
- Used to screen for disease associated with anemia and to evaluate polycythemia vera
HEMOGLOBIN DETERMINATION
serves as an important buffer in the extracellular fluid
hemoglobin
- is the ratio of the volume of packed RBCs to the volume of whole blood.
hematocrit determination
- Hematocrit is also called
PACKED CELL VOLUME (PCV)
This test indirectly measures the RBC mass.
Hematocrit determination
- Results are expressed as the ___ by volume of packed RBCs in the whole blood.
percentage or decimal liter per liter (L/L)
3 test to rule out anemia and polycythemia are
rbc count, hct and hgb determination
- blood test used to find out how many RBCs
RBC COUNT
RBC COUNT IS ALSO KNOWN AS
Erythrocyte count
WHITE BLOOD CELL COUNT ALSO CALLED AS
LEUKOCYTE COUNT)
WBCs may be counted visually using a ___ and ___
microscope and HEMACYTOMETER
wbc diluting fluid used for wbc count
hypotonic solution - in order to swell up the wbc
the ratio of diluting fluid for wbc
1:20 hypotonic
ratio of diluting fluid for rbc
1:200
A ___ detects hidden infections
WBC count
Serves as a useful guide to the severity of the disease process.
WBC count
can detect immature WBC and abnormalities, both of which are signs of potential issues.
differential count
The total count of circulating WBC is differentiated according to the five types of leukocyte
neutrophils: ___
pyogenic infection
The total count of circulating WBC is differentiated according to the five types of leukocyte
neutrophils: _ infection
eosinophils: _ infestation
basophils: _ infection
lymphocytes: _ infection
monocytes: severe infection by _
The total count of circulating WBC is differentiated according to the five types of leukocyte
neutrophils: pyogenic infection
eosinophils: allergic disorder parasitic infestation
basophils: parasitic infection
lymphocytes: viral infection
monocytes: severe infection by phagocytosis
the medium size of lymphocytes are called
robin’s egg
- Use to assess the erythropoietic activity of the bone marrow
RETICULOCYTE COUNT
blood sample for reticulocyte count
- whole blood, anticoagulated with EDTA is stained with a supravital stain such as new methylene blue or brilliant cresyl blue.
1:1 ratio
stains for retculocyte
supravital stain - such as new methylene blue or brilliant cresyl blue.
1:1 ratio of reticulocyte count sample means
one drop of blood and one drop of stain
reticulocyte incubate the sample for how any minutes?
15 mns at 37* celcius or room temperature
- Rate at which red blood cells sediment in a period of one hour
ERYTHROCYTE SEDIMENTATION RATE (ESR)
- Common hematology test and nonspecific measure of inflammation
ERYTHROCYTE SEDIMENTATION RATE (ESR)
- One of the oldest hematology tests, the ___, detects inflammation and roughly estimates its intensity
ERYTHROCYTE SEDIMENTATION RATE
- uses graduated concentrations of saline solutions to detect SPHEROCYTES (RBCs with proportionally reduced surface membrane area) in hereditary SPHEROCYTOSIS or WARM AUTOIMMUNE HEMOLYTIC ANEMIA
OSMOTIC FRAGILITY TEST
osmotic fragility test is used to detect what type of disease?
hereditary SPHEROCYTOSIS or WARM AUTOIMMUNE HEMOLYTIC ANEMIA
the capability of rbc to hold the concentration of diluting hypotonic solution
osmotic fragility test
how man test tubes are needed for the osmotic fragility test?
12
osmotic fragility test will check for the presence of
hemolysis
