week week 2 BASIC HEMATOLOGICAL METHODS OF EXAMINATION QUALITY ASSURANCE PROGRAM COLLECTION OF BLOOD ANTICOAGULANT

Question 1

COMPLETE BLOOD COUNT

Answer 1

• Basic screening test and is one of the most frequently ordered laboratory procedure.

Question 2

most commonly requested lab test

Answer 2

complete blood count

Question 3

• Gives valuable diagnostic information about the hematologic and other body system , prognosis response to treatment and recovery

Answer 3

COMPLETE BLOOD COUNT

Question 4

COMPLETE BLOOD COUNT test the following:

Answer 4

Hgb, Hct, RBC and WBC count, Differential Count

Question 5

rbc parameters

Answer 5

rbc count
hgb
hct
mcv
mchc
rdw - red distribution width
retic

Question 6

platelets parameters

Answer 6

plt count and mpv (mean platelet volume)

Question 7

if your mean platelet volume has a reference range of

Answer 7

8-10 femtoliters

Question 8

if the mpv is in 8-10 your platelet has a normal size of

Answer 8

2-4 um

Question 9

wbc parameters

Answer 9

wbc count

Question 10

the main component of erythrocytes, serves as the vehicle for the transportation of oxygen and carbon dioxide.

Answer 10

hemoglobin

Question 11

• Measurement of the concentration of Hgb in the blood

Answer 11

hemoglobin determination

Question 12

reagent used for cyanmethemoglobin method

Answer 12

Drabkin’s reagent

Question 13

• Used to screen for disease associated with anemia and to evaluate polycythemia vera

Answer 13

HEMOGLOBIN DETERMINATION

Question 14

serves as an important buffer in the extracellular fluid

Answer 14

hemoglobin

Question 15

• is the ratio of the volume of packed RBCs to the volume of whole blood.

Answer 15

hematocrit determination

Question 16

• Hematocrit is also called

Answer 16

PACKED CELL VOLUME (PCV)

Question 17

This test indirectly measures the RBC mass.

Answer 17

Hematocrit determination

Question 18

• Results are expressed as the ___ by volume of packed RBCs in the whole blood.

Answer 18

percentage or decimal liter per liter (L/L)

Question 19

3 test to rule out anemia and polycythemia are

Answer 19

rbc count, hct and hgb determination

Question 20

• blood test used to find out how many RBCs

Answer 20

RBC COUNT

Question 21

RBC COUNT IS ALSO KNOWN AS

Answer 21

Erythrocyte count

Question 22

WHITE BLOOD CELL COUNT ALSO CALLED AS

Answer 22

LEUKOCYTE COUNT)

Question 23

 WBCs may be counted visually using a ___ and ___

Answer 23

microscope and HEMACYTOMETER

Question 24

wbc diluting fluid used for wbc count

Answer 24

hypotonic solution - in order to swell up the wbc

Question 25

the ratio of diluting fluid for wbc

Answer 25

1:20 hypotonic

Question 26

ratio of diluting fluid for rbc

Answer 26

1:200

Question 27

 A ___ detects hidden infections

Answer 27

WBC count

Question 28

 Serves as a useful guide to the severity of the disease process.

Answer 28

WBC count

Question 29

 can detect immature WBC and abnormalities, both of which are signs of potential issues.

Answer 29

differential count

Question 30

 The total count of circulating WBC is differentiated according to the five types of leukocyte
 neutrophils: ___

Answer 30

pyogenic infection

Question 31

 The total count of circulating WBC is differentiated according to the five types of leukocyte
 neutrophils: _ infection
 eosinophils: _ infestation
 basophils: _ infection
 lymphocytes: _ infection
 monocytes: severe infection by _

Answer 31

 The total count of circulating WBC is differentiated according to the five types of leukocyte
 neutrophils: pyogenic infection
 eosinophils: allergic disorder parasitic infestation
 basophils: parasitic infection
 lymphocytes: viral infection
 monocytes: severe infection by phagocytosis

Question 32

the medium size of lymphocytes are called

Answer 32

robin’s egg

Question 33

• Use to assess the erythropoietic activity of the bone marrow

Answer 33

RETICULOCYTE COUNT

Question 34

blood sample for reticulocyte count

Answer 34

• whole blood, anticoagulated with EDTA is stained with a supravital stain such as new methylene blue or brilliant cresyl blue.
  1:1 ratio

Question 35

stains for retculocyte

Answer 35

supravital stain - such as new methylene blue or brilliant cresyl blue.

Question 36

1:1 ratio of reticulocyte count sample means

Answer 36

one drop of blood and one drop of stain

Question 37

reticulocyte incubate the sample for how any minutes?

Answer 37

15 mns at 37* celcius or room temperature

Question 38

• Rate at which red blood cells sediment in a period of one hour

Answer 38

ERYTHROCYTE SEDIMENTATION RATE (ESR)

Question 39

• Common hematology test and nonspecific measure of inflammation

Answer 39

ERYTHROCYTE SEDIMENTATION RATE (ESR)

Question 40

• One of the oldest hematology tests, the ___, detects inflammation and roughly estimates its intensity

Answer 40

ERYTHROCYTE SEDIMENTATION RATE

Question 41

• uses graduated concentrations of saline solutions to detect SPHEROCYTES (RBCs with proportionally reduced surface membrane area) in hereditary SPHEROCYTOSIS or WARM AUTOIMMUNE HEMOLYTIC ANEMIA

Answer 41

OSMOTIC FRAGILITY TEST

Question 42

osmotic fragility test is used to detect what type of disease?

Answer 42

hereditary SPHEROCYTOSIS or WARM AUTOIMMUNE HEMOLYTIC ANEMIA

Question 43

the capability of rbc to hold the concentration of diluting hypotonic solution

Answer 43

osmotic fragility test

Question 44

how man test tubes are needed for the osmotic fragility test?

Answer 44

12

Question 45

osmotic fragility test will check for the presence of

Answer 45

hemolysis