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Bacte Lab (Finals > Water microbiology Part 1 > Flashcards

Water microbiology Part 1 Flashcards

1
Q

Public health?

A

Portability of water
Treatment process

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2
Q

One of the qualification for RMT ?

A

Water analyst

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3
Q

Where do you train for water analyst

One of the national reference laboratory for Environmental and Occupational Health, Toxicology and Micronutrient Assa (NRL - EOHTMA)

A

East Avenue Medical Center

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4
Q

What are the standard References for Water analyst?

A

Standard Methods for the Examination of Water and Wastewater (SiMEWW)

Philippine National Standards for Drinking Water

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5
Q

Procedures intended for the examination of waters of a wider range of quality

A

Standard Methods for the Examination of Water and Wastewater

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6
Q

NOTE: For SIMEWW

A

Covers:
- Domestic or industrial supplies
- Surface water
- Ground Water
- Cooling or Circulating water
- Boiler water
- Boiler feed water
- Treated and untreated municipal or industrial wastewater
- Saline water

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7
Q

Water comes from industrial factories that uses for cleaning, etc.

A

Wastewater

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8
Q

Philippine National Standards for Drinking Water is also known as?

A

Administrative order 2017-0010

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9
Q

Standards have been set by Philippines DOH in order to ensure that the general public is given access to safe drinking water

A

Philippine national Standards for Drinking water

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10
Q

4 Water analysis

A

Physical
Chemical
Microbiological
Radiological

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11
Q

Water analysis that determines temp, turbidty, etc

A

Physical

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12
Q

Water analysis that determines the amount of reagents such as reagents, etc

A

Chemical

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13
Q

Water analysis that determines the amount of microbes in the water?

A

Microbiological

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14
Q

Water analysis that determine the radio isotopes in the water?

A

Radiological

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15
Q

NOTE: PNSDW

A

free from contamination by human and
animal excreta

indices of potential water borne diseases

The major interest in classifying and issuing standards is the
identification, quantification, and evaluation of organisms associated with
waterborne diseases.

Frequent examination for fecal indicator organisms remain as the most
sensitive and specific way of assessing the hygienic quality of water.

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16
Q

major interest in classifying and issuing standards is?

A

Identification
Quantification
Evaluation of organisms

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17
Q

Most sensitive and specific

Provides evidence of fecal contamination from humans or warm-blooded animals

A

Indicator organism

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18
Q

Criteria for indicator Organisms

A

Always present

Large numbers in the feces

Respond to natural environmental conditions

Readily detectable

Ratio of indicator/pathogen should be high

Comes from the same source (GIT)

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19
Q

NOTE:

A

“No organism fulfills all the criteria for an indicator organism, but the coliform bacteria fulfill most”

20
Q

What Family of bacteria is can be considered coliform group?

A

Enterobacteriaceae

21
Q

Incubation for fermentation of Enterobacteriaceae?

A

35 C within 48 hrs

22
Q

What is the indicator organism for Coliform bacteria?

A

Escherichia coli

23
Q

What is the characteristics for Enterobacteriaceae?

A

Facultatively anaerobic
Gram (-)
Non spore forming
Rod shaped
Bacteria that ferment lactose with gas
acid formation

24
Q

Routine analysis for Drinking water

A

Heterotrophic plate Count
Coliform count (Total and Thermotolerant Coliform count)

25
Q

Describes a broad group of bacteria that include pathogens nonpathogens and opportunistic microorganisms

A

Heterotrophic bacteria

26
Q

indicate general biological condition of drinking water as a consequence of insufficiency of treatment process, regrowth or recontamination of drinking water in the distribution system

A

Heterotrophic plate count

27
Q

3 Procedures for heterotrophic plate count?

A

Pour plate
Spread plate
Membrane Filtration technique

28
Q

Subgroup of total coliforms that are differentiated using elevated temperature

A

Thermotolerant fecal coliforms

29
Q

What is the temp for thermotolerant fecal coliforms?

A

43 - 44.5 C

30
Q

Collection container:

A

Nonreactive borosilicate glass or plastic bottles
(Final rinse: Deionized / Distilled water)
Sterilized: 121C in 15mins

31
Q

The containers must be able to contain at least__________ with additional space to facilitate
mixing.

A

250 mL

32
Q

A minimum amount of _________ is required if
samples are to be tested for presence of total
coliform, thermotolerant coliform and
heterotrophic bacteria.

A

150 mL

33
Q

NOTE: Bottles must be unopened and kept away from
contamination until it is required for filling.
During filling the stopper and the cap must be
removed as a unit

A
34
Q

A __________ is added to collection
containers of water having residual chlorine or
other halogens

A

reducing agent

(Dechlorination)

35
Q

What is the reducing agent the neutralized any residual halogen and prevents continuation of bactericidal action during sample transit?

A

Sodium thiosulfate

36
Q

What is the concentration for Sodium thiosulfate to neutralize 5 mg/L residual chlorine?

A

0.1 mL of a 3% solution

37
Q

How to transport samples?

A

Zip locked bags that contains wet ice (10C)

38
Q

What is the holding time for coliform count?

A

30 hrs

39
Q

What is the holding time for heterotrophic plate count?

A

8 hrs

40
Q

Temp for storage?

A

2-4C

41
Q

What is the retention time for storage?

A

Month or longer

42
Q

What is the standard hold time for both coliform and heterotrophic plate count in the Philippines?

A

24 hrs

43
Q

Label:

A

Location
Treatment for water
Date of colletion
Time of collection
Who collected

44
Q

What water is use for the final rinse before sterilizing the nonreactive borosilicate glass

A

Distilled or deionized water

45
Q

What is the holding time for storage if positive?

A

1 month

46
Q

What is the holding for storage if negative?

A

3 days

47
Q

Procedure in collecting water

A
  1. Aseptically clean the faucet (Bleach then alcohol)
  2. Flame the tip of the faucet
  3. leave the running water for 1 min
  4. Collect the water (Make sure to leave a space on the top)
  5. Label
  6. Transport (10C) - Retention (HPC: 30 hrs, CC: 8 hrs)
  7. Storage (2-4 C)

Bacte Lab (Finals (13 decks)

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