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Bacte Lab (Finals > Kirby bauer > Flashcards

Kirby bauer Flashcards

1
Q

To give information to physicians on the proper antibiotics that will give to the patient

To test the effectiveness of antibiotics

A

AST

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2
Q

AST comes with?

A

Culture

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3
Q

What are the methods of AST?

A

Diffusion
Dilution method

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4
Q

Examples of Diffusion method?

A

Kirby bauer
Agar cup diffusion method (Liquid)
Agar cylinder diffusion method (Liquid)
Epsilometer test

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5
Q

Most common medium for kirby bauer diffusion method?

A

Mueller hinton agar

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6
Q

Uses antibiotic in strips

A

Epsilometer test

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7
Q

Method of AST that is commonly used in research

A

Dilution method (Macro and Micro)

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8
Q

Generally, what is the incubation for kirby bauer?

A

18-24hrs

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9
Q

What is the measuring tool for measuring of zone of inhibition in actual practice?

A

Vernier caliper

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10
Q

Components of McFarland

A

99.5 mL of 1% sulfuric acid
0.5mL of 1.175% barium chloride

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11
Q

NOTE: Difference of McFarland differs in the amount of components

A
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12
Q

What is the optical density for McFarland?

A

600nm with an absorbance of 0.08 to 0.1

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13
Q

Research study have a protocol for McFarland, what is the nm?

A

625nm

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14
Q

Example of densitometer?

A

Sensititre Nephelometer

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15
Q

The green light in Sensititre nephelometer indicates?

A

0.5 McFarland/Density

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16
Q

The red light on the right side of the green light in Sensititre nephelometer indicates?

A

High density

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17
Q

The red light on the Left side of the green light in Sensititre nephelometer indicates?

A

Add NSS or Demineralized water

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18
Q

Sensititre nephelometer usually used what water in red light on the left?

A

Demineralized water

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19
Q

What is the concentration of NSS used in the Experiment?

A

0.85 - 0.9%

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20
Q

How many colonies from plated media should be put in the MHA?

A

4-5

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21
Q

What is the pH for MHA?

A

7.2 to 7.4

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22
Q

What is the depth of MHA?

A

5mm

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23
Q

NOTE: After prep of MHA, ref first then incubate for 10 - 20 mins To dry off moisture

A
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24
Q

What organisms can be used for MHA only?

A

Non-fastidious organisms

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25
What organisms can be used for MHA + 5%NaCl?
Staphylococcus spp. MRSA
26
What organisms can be used for MHA + 5% defibrinated blood sheep?
Haemophilus spp (Fastidious) Streptococci spp (Hemolysis)
27
What is the size of plate in AST for 12 antibiotics?
150mm x 15mm (Big plate)
28
What is used for streaking the inoculum in AST?
Sterile cotton Swab
29
How to Streak in AST?
Vertical and Diagonal streaking (No Space)
30
How many mins to stand in AST after streaking?
3 - 5 mins
31
How many antibiotics should be impregnated in a paper disk ?
12 different antibiotics
32
What is used to impregnated the antibiotics in a paper disk?
Sterile forceps
33
How many antibiotics in outer part?
8
34
How many antibiotics in Inner part?
4
35
What is the proper spacing in between antibiotics?
>15 mm
36
In 100mm plate (Small petri dish), how many antibiotics can fit in this plate?
4-6 antibiotics
37
What family of bacteria use in big plate for the detection of ESBL (Extended spectrum beta lactamases) and AmpC (Ampicillinase C)
Enterobacteriaceae
38
What are the 6 antibiotics for Enterobacteriaceae
FOX ATM AMC CAZ CTX IPM
39
What are the antibiotics used for Staph and Strep?
Erythromycin Clindamycin (In the Middle)
40
How many hours for incubation for Enterobacteriaceae?
16-18hrs
41
How many hours for incubation for P. aeruginosa??
16-18 hrs
42
How many hours for Enterococci with vancomycin Resistant?
24 hrs
43
How many hours for Staphylococcus with Vancomycin and Methicillin resistant?
24 hours
44
What is the incubation time for Strep?
20 - 24 hrs
45
What is the incubation time for Neisseria ?
20 - 24 hrs with 5-10% CO2
46
What is the incubation time for H. influenzae ?
16-18 hrs with 5-10% CO2
47
Indicates an Organism is inhibited by the recommended dose
Susceptible
48
Indicates an organism may require a higher dose for longer period of time
Intermediate
49
Indicates an organism is not inhibited by recommended dose
Resistance
50
What is the unit for Zone of inhibition?
millimeters
51
What happens when Inoculum is too light and Nutritionally poor medium?
Zone is large
52
What to do if the inoculum is too light?
Adjust the inoculum to McFarland 0.5 standard turbidity
53
What to do Nutritionally poor medium?
Only use MHA
54
What happens to the zone there is slow growing organisms and improper depth (Less than <5)?
Zone is large
55
What to do if there is slow growing organisms
Use Minimum inhibitory concentration (MIC) only
56
What happen to the zone is the inoculum is too heavy and improper depth >5?
Zone is small
57
Reason for colonies in the zone of inhibition?
Mixed culture Resistant mutants within the zone
58
What to do if there is mixed culture?
Isolate, identify and retest pure culture
59
What to do if there is resistant mutants within the zone?
Gram stain Repeat test
60
Reason for zones that are overlap?
Disk too close (No more than 12 disk (Big Plate) or 4-6 disk (Small plate), not <15mm)
61
Reason for zone indisticnt with single colonies noted on the plate
Not proper streaking Inadequate inoculum
62
Should Mycobacterium use disk method?
FALSE
63
What is the method used for Mycobacterium?
Dilution and broth method
64
What is the media used for Mycobactrium in AST?
Middle brook 7H10
65
Employs boring holes on the agar while streaking of inoculum on agar is one in an overlapping manner?
Agar cup diffusion method
66
Antibiotics in Agar cup diffusion method are in what form?
Liquid
67
Makes us of a metal or plastic cylinder
Agar cylinder diffusion method
68
Can used to detect Minimum inhibitory concentration (MIC) Plastic strips is impregnated
Epsilometer test
69
How to read Epsilometer?
Intercets on the strip on Zone inhibition
70
What is being diluted in AST?
Antibiotic NOT bacteria

Bacte Lab (Finals (13 decks)

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