Dilution method

Question 1

How many mL for macrodilution test?

Answer 1

1mL broth

Question 2

How many mL for microdilution test?

Answer 2

0.05mL to 0.1mL

Question 3

For microorganisms with unpredictable susceptibilities

Answer 3

Minimum inhibitory concentration

Question 4

NOTE: MIC is the first broth in which the growth of the organisms has been inhibited

EX: (CLick again)

Answer 4

1st tube (10mg): No growth
2nd tube (20mg): No growth
3rd tube (30 mg): Growth

Question 5

The more resistant an organism, the ______ will be the MIC. (Higher/Lower)

Answer 5

Higher

Question 6

Set up for Macrobroth?

Answer 6

MHB + Antibiotics + Inoculums of uniform volume (Same volume)

Question 7

Antibiotics are NOT uniformed but?

Answer 7

Serially diluted

Question 8

This system uses a tray that would contain the antibiotics

Answer 8

Alamar system

Question 9

What is the indicator for Alamar system?

Answer 9

Alamar Blue indicator

Question 10

Set up of alamar system?

Answer 10

MHB + ORganism + Alamar blue indicator

Question 11

What is the indicator when there is growth?

Answer 11

Blue to pink

PINK

Question 12

What is used in microdilution?

Answer 12

Microtiter plate

Question 13

Uses automated multi-pronged inoculators calibrated to deliver the precise volume of inoculum to each well

Answer 13

Microdilution

Question 14

What is the temp for the storage in microdilution?

Answer 14

• 20C

Question 15

Once thawed, the panels should never be refrozen because of substantial loss of antimicrobial action and potency? T or F

Answer 15

TRUE

Question 16

Instead of freezing microdilution, what is the alternative for this?

Answer 16

Lyophilization

Question 17

Detect growth in micro volumes of broth with various dilutions of antimicrobials

Detection via photo, turbid, or fluorometric methods

Answer 17

Automated AST methods

Question 18

Automated AST methods machines?

Answer 18

BD phoenix
Microscan walkaway
TREK sensititre
Vitek 1 and 2

Question 19

What are the advantages of Automated AST methods?

Answer 19

Increased reproductivity
Decreased labor cost
Rapid
Software
Detects multi-drug resistance
ESBLs
Correlates bacterial ID and sensitivity

Question 20

What is the only disadvantage of Automated AST methods?

Answer 20

Cost