Medical Micro

Question 1

Prior to performing gram staining?

Answer 1

Smear prep

Question 2

NOTE:

Swab is role in on the slide

Urine is aspirate by the use of pipette or loos

Question 3

Smear prep

Answer 3

1. Heat / Methanol Fixation
2. Gram staining

Question 4

NOTE: the timing of applying and wating the reagent depends on the manufacturer

Question 5

Responsible for syphilis or inta

Answer 5

Treponims / Treponema spp.

Question 6

NOTE: Blood agar plate = Hemolysis

Answer 6

Beta, Alpha, Gamma

Question 7

NOTE: MacConkey Agar

Answer 7

Selective: Only gram neg grows
Differential: Differentiates Gram neg by lactose fermenting

Question 8

NOTE: SIM

Answer 8

S- sulfide (Blackening
I - Indole
M - Motility (Punture)

Question 9

NOTE: Citrate

Answer 9

Blue: Positive
Green: Negative

Question 10

NOTE: TSI

Answer 10

3 sugars
- Sucrose and Lactose (Slant)
- Glucose (Butt)

Question 11

H2S is a 2 way process:

Question 12

Indiator for H2S?

Answer 12

Iron

Question 13

Ferric ions + H2S = ?

Answer 13

Ferrous sulfide (Black precipitate)

Question 14

Common morphology: Pseudomonas aeruginosa

Answer 14

Blue - Green Pigment (MHA, MAC)

Question 15

Common morphology: Serratia marcescens

Answer 15

Red pigment

Question 16

Klebsiella and Raoutella spp are Motile or non?

Answer 16

Non