Viral Replication

Question 1

Viral growth different from bacterial because

Answer 1

Eclipse period - time between entry time and progeny creation…there is time where it is just viral DNA in the cell

Question 2

dsDNA virus replication and transcription

Answer 2

Transcribe genes using host RNA polymerase
Replication using host DNA polymerase

Use proteins and progeny DNA to package and export

Question 3

Capsid proteins must

Answer 3

Enter the nucleus after being synthesized

Question 4

When do viruses acquire spikes and final envelope?

Answer 4

Golgi

Question 5

Examples of dsDNA viruses that are important

Answer 5

Herpes

Question 6

Inportant ssRNA+ naked

Answer 6

Entero, Rhino, Polio

Question 7

ssRNA+ replication

Answer 7

Translated by host ribosomes
Synthesize RNA polymerase (RDRP)…this then creates ssRNA-…this is template for ssRNA+ used to package

ssRNA+ also used to translate proteins for capsid

Question 8

Hep A

Answer 8

ssRNA+ naked virus

Question 9

VPg protein

Answer 9

Primes RDRP synthesis of RNA in Hep A

Question 10

Replicaiton of ssRNA- genomes

Answer 10

Contains an RDRP (already made)
This synthesizes ssRNA+ strands used to do 2 things
1) Make viral proteins including RDRP
2) Use as template to make more ssRNA- to be packaged

Question 11

Example of ssRNA- genomes

Answer 11

Ebolavirus

Question 12

Influenza virus genome and structure

Answer 12

8 ssRNA- enveloped

Question 13

CM of influenze

Answer 13

Abrupt (important) onset of fever, chills, non-productive cough and muscle aches (from cytokines)

Question 14

Mech of influenza

Answer 14

HA - binds virus to host cell via sialic acid and triggers endocytosis
NA - hydrolyzes sialic acid to release

These are both glycoprotein spikes in the envelope