Urine Cultures Flashcards
Organisms commonly isolated from UTIs
E.coli, Enterococcus, Staph saprophyticus, Staph aureus, Pseudomonas aeruginosa, Strep agalactiae, Strep pyogenes, Candida
A 0.001 mL loop represents how many colony forming units in urine?
1,000 col/mL
A 0.01 mL loop represents how many colony forming units in the urine
100 col/mL
When 3 or more organisms are each >100,000 col/mL, what should be suspected
Contamination & long term sitting @ room temp
3 or more colony types each less than 10,000 col/mL, what should be suspected?
Contaminants
1 colony type between 10,000-50,000 col/mL, what should be interpreted if sample came from general clinic, hospital patient, urology clinic?
General clinic: likely no treatment - contaminant
Hospital patient: likely no treatment - contaminant
Urology clinic: depending on diagnosis
1 colony type between 50-100,000 col/mL, what is the interpretation if sample comes from general clinic, hospital patient, urology clinic?
General clinic: probable treatment depending on symptoms, WBC’s, etc
Hospital patient: treatment likely
Urology clinic: treatment likely
1 colony type >100,000 col/mL, what is the interpretation if sample comes from general clinic, hospital patient, urology clinci
General clinic: treatment
Hospital patient: treatment
Urology clinic: treatment
Reasons why 10,000 col/mL may be significant
Chronic condition, unique diagnostic problems, immunocompromised patient
Clean catch midstream urine collection
Patient cleanses area, begins voiding in toilet for discard, then collects rest of specimen
Pathogenicity of Staph saprophyticus in urine cultrues
Problem with infertility in child bearing aged women, also cause UTI
Staph saprophyticus ID
Cat +
Coag =
resistant to novobiocin,
C biochemicals
Pyelonephritis
Infection located in the renal parenchyma
Cystitis
Infection located in the bladder
Pyuria
WBC’s in urine
