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Microbiology 484 > Media > Flashcards

Media Flashcards

1
Q

Regular/ambient environment

A

35-37 degree

room atmosphere

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2
Q

Increased CO2 environment

A

Increased CO2 to 10% (ambient air CO2 is 1%)

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3
Q

anaerobic environment

A

All O2 removed

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4
Q

Microaerophilic environment

A

Orgs require partial pressure of O2 less that atmospheric oxygen (21% O2)

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5
Q

Principle of Gas pack jar

A

H20 is added to generator and hydrogen & carbon dioxide gasses are produced

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6
Q

Principle of anaerobic bags

A

O2 removal system is initiated

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7
Q

Principle of anaerobic glove box

A

Vinyl is surrounding an area large enough for plates, specimens etc. The tech inserts hands into gloves inside of vinyl tent to prevent any O2 from entry

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8
Q

Capnophilic

A

Microorganisms that require an increased concentration of CO2, usually between 5-10%

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9
Q

Halophlic

A

“Salt-loving”

An organism that grows best in media with increased concentration of NaCL

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10
Q

Psychrophiles

A

Bacteria that grow best @ cold temps (10-20 degrees C)

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11
Q

Mesophiles

A

Organisms that grow best in moderate temp (20-40 degree c)

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12
Q

Thermophiles

A

Bacterial that grow best @ high temps (50-60 degree C)

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13
Q

Cary-Blair medium

A

Allows recovery of Salmonellae, shigella, vibrio cholerae, Campylobacter

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14
Q

Culturette

A

Sterile specimen collection system that has a polyester swab & plastic shaft. Transport media is in a glass ampule which is broken after the specimen has been put on the swab to moisten it

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15
Q

Calgiswab

A

Calcium alginate wool on flexible aluminum nasopharyngeal swab

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16
Q

Stuart’s or Amie’s medium

A

viral transport media

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17
Q

Autoclave sterilization

A

121.6 degree c for 15 minutes @ 15 psi

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18
Q

Filtration sterilization

A

0.22 um pore size for solutions, 0.30 um for size pores for HEPA filters

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19
Q

Dry heat sterilization

A

160-180 degrees C for 1.5-3 hours

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20
Q

Gas sterilization f

A

Ethylene oxide

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21
Q

Radiation

A

Ionizing radiation of gamma rays or electron beams

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22
Q

Purpose of gram stain

A

to make visible the bacteria and determine gram stain reaction - positive or negative & morphology - cocci or rod or other

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23
Q

Gram positive bacteria

A

bacteria with thick cell walls containing teichoic acid retain the crystal violet-iodine complex dye after decolorization

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24
Q

Gram negative bacteria

A

Bacteria with thinner walls containing lipopolysaccharides don’t retain the dye complex & pick up counterstain

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25
Function of crystal violet
Purple stain that attaches to bacterial cell wall | Primary stain
26
Function of iodine-mordent
Drives crystal violet into cell
27
Function of decolorizer
Alcohol or acetone alcohol | removes crystal violet form cell walls with high lipid content
28
Purpose of Safranin
Stains cell walls with high lipid content red | Secondary stain
29
Selective media
Supports the growth of one type or group of microbes while inhibiting the growth of others Ex. Mac plate
30
Differential media
Helps group bacteria by certain characteristics displayed by the media Ex. Mac, HE, XLD, EMB
31
Enriched media
Nonselective & supports growth of almost all fastidious & non-fastidious microbes Ex. CAP
32
Enrichment broth
Helps support a minority population of bacteria grow while suppressing another Ex. Salmonella/Shigella broth
33
Sheep Blood Agar (BAP) purpose, class, components
routine media nonselective Tryptic soy agar, 5-10% sheep blood
34
Phenylethanol Blood Agar (PEA) purpose, class, components
Selective for gram pos cocci Selective media Phenyl ethyl alcohol inhibits aerobic neg rods
35
Chocolate Agar (GC medium base) purpose, class, components
Promotes growth of haemophilus & other fastidious organisms Enrichment media Contains cell-free Hgb & NAD and sheep blood
36
CNA purpose, class, components
Isolation of cocci Selective, enrichment media Enriched with sheep blood & has antibiotics (colistin & naladistic acid to inhibit neg rods). Tri prevents proteus from swarming. Nystatin inhibits fungus
37
EMB purpose, class, components
Isolation of gram neg rod Selective, differential media E. coli - blue black colonies Eosin Y & methylene blue decrease +C growth
38
MacConkey purpose, class, components
Isolate gram neg rods Selective, differential Bile salts & crystal violet inhibits pos
39
XLD purpose, class, components
Isolate & recognize lactose neg & H2S + orgs selective, differential Shows lactose =, H2S production
40
Salmonella-Shigella Agar (SS) purpose, class, components
Isolate Salmonella & Shigella (not as well) Selective, differential Non lactose fermenters & H2S production
41
Tergitol-7 agar (Te-7)purpose, class, components
To identify lactose negative for Salmonella & Shigella isolation Selective, differential Lactose = orgs are visible
42
Hektoen Agar purpose, class, components
To identify lactose = for Salmonella & Shigella iso Selective, differential Lactose = orgs are visible
43
Campy-BAP purpose, class, components
Selective for Campylobacter species Selective, enriched Brucella agar base, antibiotics & sheep blood
44
Modified Thayer Martin purpose, class, components
Selection for Neisseria & inhibits for NF Selective, enriched Added growth factors, Vacomycin inhibits +C, colistin inhibits = rods
45
HBT/V agar purpose, class, components
Isolate Garderella vaginalis Non-selective, enriched Human blood will produce beta hemolysis
46
SSA/SXT purpose, class, components
Isolate S. pyogenes & S. agalactiae Selective Bile salts & sodium thio sulfate inhibit gp & gn rods
47
Anaerobic PEA | purpose, class, components
Helps gp & gn nonsporalating anaerobes Selective Add PEA to CDC anaerobic agar
48
Anaerobic KV purpose, class, components
Isolation of obligate anaerobes, particularly Bacteroides Selective Addition of Kanamycin & vancomycin to anaerobic BAP
49
Campy thio purpose, class, components
Allows Campylobacter to grow Selective enrichment Brucella agar base, 10% antibiotic mixture
50
Thioglycolate purpose, class, components
Helps small #s of orgs to grow, will support anaerobes All purpose broth Thioglycolate, cysteine, sodium sulfite are reducing agents
51
GN broth purpose, class, components
Enhance the growth of Salmonella/Shigella Selective, enriched Deoxycholate & cirate salts decrease growth of + orgs, increase mannitol to increase Salmonella/Shigella
52
Function of indicator in thio media
Color indicates if the O2 has been eliminated to support the growth of anaerobes. Boiling reducing agents will drive O2 of the top
53
Mac with Sorbitol used to isolate?
E. coli O157:H7
54
Mannitol Salt agar used to isolate?
Staph aureus
55
Tinsdale used to isolate?
Corynebacterium diphtheriae
56
Lowenstein-Jensen used to isolate?
Mycobacteria
57
Bismuth Sulfite (BS) used to isolate?
Salmonella typhi & other Salmonella
58
Middlebrook 7H-10 used to isolate?
Mycobacterium
59
Fletcher's medium used to isolate?
Leptospira
60
BCYE (buffered charcoal-yeast extract agar) used to isolate?
Legionella
61
TCBS (thio sulfate citrate bile salts sucrose) used to isolate?
Vibrio
62
Percentage of blood used in preparing SBA
5%
63
Strep pyogenes isolated on what media?
SSA
64
Staph aureus isolated on what media?
Mannitol Salt agar
65
Corynebacterium diphtheria isolated on what media?
Tinsdale
66
Bacteroides fragilis isolated on what media?
anaerobic KV
67
E. coli isolated on what media?
EMB, Mac
68
Neisseria gonorrhoeae isolated on what media?
MTM
69
Salmonella typhi isolated on what media?
HE, XLD, TE-7
70
Shigella sonneiisolated on what media?
HE, XLD, TE-7
71
Clostridium perfringens isolated on what media?
BRU/BA (Brucella/Blood agar plate or Egg yolk agar)
72
Vibrio chlera isolated on what media?
TCBS
73
Vibrio parahemolyticus isolated on what media?
TCBS
74
Campylobacter jejuni isolated on what media?
Campy BAP
75
Regulations for mailing biologicals
The biological must be placed in a securely closed, watertight vial or test tube surrounded by absorbent material, then placed in a labeled secondary container
76
If there is growth on BAP, Choc, CNA...
....the direct gram stain could be gram pos cocci
77
If there is growth on Mac only...
...the direct gram stain could be gram neg rod
78
If there is growth on the Choc plate only...
...could indicated fastidious organisms such as Neisseria (gram neg diplococcic) and Haemophilus (tiny gram neg rod)
79
If Staph aureus is present...
...then growth on BAP, CAP, CNA
80
If Haemophilus influenza is present...
...then growth on CAP
81
If bacillus spp is present...
...then growth on BAP, CAP
82
If Enterobacter aerogenes is present...
...then growth on BAP, CAP, MAC
83
If Strep pyogenes & Cornybacterium spp present...
...then growth on BAP, CAP, CNA
84
If strep paneumoniae present...
...then growth on BAP, CAP, CNA
85
If Candida albicans present...
...then growth on BAP, CAP
86
Level 1 BSL
Agents are well classified and are not known to cause disease consistently in healthy adults
87
Level 2 BSL
Agents that pose a moderation potential hazard for the employees & the environment
88
Level 3 BSL
Agents that are indigenous or exotic, potential for aerosol transmission & may have serious lethal consequences
89
Level 4 BSL
``` Agents that are dangerous & exotic, high risk of life-threatening infections, aerosols, unknown risk of transmission -PPE, BSC class III, neg pressure rooms, decontamination of room/personnel ```

Microbiology 484 (15 decks)

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