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Microbiology 484 > Media > Flashcards

Media Flashcards

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1
Q

Regular/ambient environment

A

35-37 degree

room atmosphere

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2
Q

Increased CO2 environment

A

Increased CO2 to 10% (ambient air CO2 is 1%)

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3
Q

anaerobic environment

A

All O2 removed

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4
Q

Microaerophilic environment

A

Orgs require partial pressure of O2 less that atmospheric oxygen (21% O2)

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5
Q

Principle of Gas pack jar

A

H20 is added to generator and hydrogen & carbon dioxide gasses are produced

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6
Q

Principle of anaerobic bags

A

O2 removal system is initiated

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7
Q

Principle of anaerobic glove box

A

Vinyl is surrounding an area large enough for plates, specimens etc. The tech inserts hands into gloves inside of vinyl tent to prevent any O2 from entry

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8
Q

Capnophilic

A

Microorganisms that require an increased concentration of CO2, usually between 5-10%

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9
Q

Halophlic

A

“Salt-loving”

An organism that grows best in media with increased concentration of NaCL

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10
Q

Psychrophiles

A

Bacteria that grow best @ cold temps (10-20 degrees C)

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11
Q

Mesophiles

A

Organisms that grow best in moderate temp (20-40 degree c)

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12
Q

Thermophiles

A

Bacterial that grow best @ high temps (50-60 degree C)

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13
Q

Cary-Blair medium

A

Allows recovery of Salmonellae, shigella, vibrio cholerae, Campylobacter

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14
Q

Culturette

A

Sterile specimen collection system that has a polyester swab & plastic shaft. Transport media is in a glass ampule which is broken after the specimen has been put on the swab to moisten it

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15
Q

Calgiswab

A

Calcium alginate wool on flexible aluminum nasopharyngeal swab

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16
Q

Stuart’s or Amie’s medium

A

viral transport media

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17
Q

Autoclave sterilization

A

121.6 degree c for 15 minutes @ 15 psi

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18
Q

Filtration sterilization

A

0.22 um pore size for solutions, 0.30 um for size pores for HEPA filters

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19
Q

Dry heat sterilization

A

160-180 degrees C for 1.5-3 hours

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20
Q

Gas sterilization f

A

Ethylene oxide

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21
Q

Radiation

A

Ionizing radiation of gamma rays or electron beams

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22
Q

Purpose of gram stain

A

to make visible the bacteria and determine gram stain reaction - positive or negative & morphology - cocci or rod or other

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23
Q

Gram positive bacteria

A

bacteria with thick cell walls containing teichoic acid retain the crystal violet-iodine complex dye after decolorization

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24
Q

Gram negative bacteria

A

Bacteria with thinner walls containing lipopolysaccharides don’t retain the dye complex & pick up counterstain

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25
Q

Function of crystal violet

A

Purple stain that attaches to bacterial cell wall

Primary stain

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26
Q

Function of iodine-mordent

A

Drives crystal violet into cell

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27
Q

Function of decolorizer

A

Alcohol or acetone alcohol

removes crystal violet form cell walls with high lipid content

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28
Q

Purpose of Safranin

A

Stains cell walls with high lipid content red

Secondary stain

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29
Q

Selective media

A

Supports the growth of one type or group of microbes while inhibiting the growth of others
Ex. Mac plate

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30
Q

Differential media

A

Helps group bacteria by certain characteristics displayed by the media
Ex. Mac, HE, XLD, EMB

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31
Q

Enriched media

A

Nonselective & supports growth of almost all fastidious & non-fastidious microbes
Ex. CAP

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32
Q

Enrichment broth

A

Helps support a minority population of bacteria grow while suppressing another
Ex. Salmonella/Shigella broth

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33
Q

Sheep Blood Agar (BAP) purpose, class, components

A

routine media
nonselective
Tryptic soy agar, 5-10% sheep blood

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34
Q

Phenylethanol Blood Agar (PEA) purpose, class, components

A

Selective for gram pos cocci
Selective media
Phenyl ethyl alcohol inhibits aerobic neg rods

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35
Q

Chocolate Agar (GC medium base) purpose, class, components

A

Promotes growth of haemophilus & other fastidious organisms
Enrichment media
Contains cell-free Hgb & NAD and sheep blood

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36
Q

CNA purpose, class, components

A

Isolation of cocci
Selective, enrichment media
Enriched with sheep blood & has antibiotics (colistin & naladistic acid to inhibit neg rods). Tri prevents proteus from swarming. Nystatin inhibits fungus

37
Q

EMB purpose, class, components

A

Isolation of gram neg rod
Selective, differential media
E. coli - blue black colonies
Eosin Y & methylene blue decrease +C growth

38
Q

MacConkey purpose, class, components

A

Isolate gram neg rods
Selective, differential
Bile salts & crystal violet inhibits pos

39
Q

XLD purpose, class, components

A

Isolate & recognize lactose neg & H2S + orgs
selective, differential
Shows lactose =, H2S production

40
Q

Salmonella-Shigella Agar (SS) purpose, class, components

A

Isolate Salmonella & Shigella (not as well)
Selective, differential
Non lactose fermenters & H2S production

41
Q

Tergitol-7 agar (Te-7)purpose, class, components

A

To identify lactose negative for Salmonella & Shigella isolation
Selective, differential
Lactose = orgs are visible

42
Q

Hektoen Agar purpose, class, components

A

To identify lactose = for Salmonella & Shigella iso
Selective, differential
Lactose = orgs are visible

43
Q

Campy-BAP purpose, class, components

A

Selective for Campylobacter species
Selective, enriched
Brucella agar base, antibiotics & sheep blood

44
Q

Modified Thayer Martin purpose, class, components

A

Selection for Neisseria & inhibits for NF
Selective, enriched
Added growth factors, Vacomycin inhibits +C, colistin inhibits = rods

45
Q

HBT/V agar purpose, class, components

A

Isolate Garderella vaginalis
Non-selective, enriched
Human blood will produce beta hemolysis

46
Q

SSA/SXT purpose, class, components

A

Isolate S. pyogenes & S. agalactiae
Selective
Bile salts & sodium thio sulfate inhibit gp & gn rods

47
Q

Anaerobic PEA

purpose, class, components

A

Helps gp & gn nonsporalating anaerobes
Selective
Add PEA to CDC anaerobic agar

48
Q

Anaerobic KV purpose, class, components

A

Isolation of obligate anaerobes, particularly Bacteroides
Selective
Addition of Kanamycin & vancomycin to anaerobic BAP

49
Q

Campy thio purpose, class, components

A

Allows Campylobacter to grow
Selective enrichment
Brucella agar base, 10% antibiotic mixture

50
Q

Thioglycolate purpose, class, components

A

Helps small #s of orgs to grow, will support anaerobes
All purpose broth
Thioglycolate, cysteine, sodium sulfite are reducing agents

51
Q

GN broth purpose, class, components

A

Enhance the growth of Salmonella/Shigella
Selective, enriched
Deoxycholate & cirate salts decrease growth of + orgs, increase mannitol to increase Salmonella/Shigella

52
Q

Function of indicator in thio media

A

Color indicates if the O2 has been eliminated to support the growth of anaerobes. Boiling reducing agents will drive O2 of the top

53
Q

Mac with Sorbitol used to isolate?

A

E. coli O157:H7

54
Q

Mannitol Salt agar used to isolate?

A

Staph aureus

55
Q

Tinsdale used to isolate?

A

Corynebacterium diphtheriae

56
Q

Lowenstein-Jensen used to isolate?

A

Mycobacteria

57
Q

Bismuth Sulfite (BS) used to isolate?

A

Salmonella typhi & other Salmonella

58
Q

Middlebrook 7H-10 used to isolate?

A

Mycobacterium

59
Q

Fletcher’s medium used to isolate?

A

Leptospira

60
Q

BCYE (buffered charcoal-yeast extract agar) used to isolate?

A

Legionella

61
Q

TCBS (thio sulfate citrate bile salts sucrose) used to isolate?

A

Vibrio

62
Q

Percentage of blood used in preparing SBA

A

5%

63
Q

Strep pyogenes isolated on what media?

A

SSA

64
Q

Staph aureus isolated on what media?

A

Mannitol Salt agar

65
Q

Corynebacterium diphtheria isolated on what media?

A

Tinsdale

66
Q

Bacteroides fragilis isolated on what media?

A

anaerobic KV

67
Q

E. coli isolated on what media?

A

EMB, Mac

68
Q

Neisseria gonorrhoeae isolated on what media?

A

MTM

69
Q

Salmonella typhi isolated on what media?

A

HE, XLD, TE-7

70
Q

Shigella sonneiisolated on what media?

A

HE, XLD, TE-7

71
Q

Clostridium perfringens isolated on what media?

A

BRU/BA (Brucella/Blood agar plate or Egg yolk agar)

72
Q

Vibrio chlera isolated on what media?

A

TCBS

73
Q

Vibrio parahemolyticus isolated on what media?

A

TCBS

74
Q

Campylobacter jejuni isolated on what media?

A

Campy BAP

75
Q

Regulations for mailing biologicals

A

The biological must be placed in a securely closed, watertight vial or test tube surrounded by absorbent material, then placed in a labeled secondary container

76
Q

If there is growth on BAP, Choc, CNA…

A

….the direct gram stain could be gram pos cocci

77
Q

If there is growth on Mac only…

A

…the direct gram stain could be gram neg rod

78
Q

If there is growth on the Choc plate only…

A

…could indicated fastidious organisms such as Neisseria (gram neg diplococcic) and Haemophilus (tiny gram neg rod)

79
Q

If Staph aureus is present…

A

…then growth on BAP, CAP, CNA

80
Q

If Haemophilus influenza is present…

A

…then growth on CAP

81
Q

If bacillus spp is present…

A

…then growth on BAP, CAP

82
Q

If Enterobacter aerogenes is present…

A

…then growth on BAP, CAP, MAC

83
Q

If Strep pyogenes & Cornybacterium spp present…

A

…then growth on BAP, CAP, CNA

84
Q

If strep paneumoniae present…

A

…then growth on BAP, CAP, CNA

85
Q

If Candida albicans present…

A

…then growth on BAP, CAP

86
Q

Level 1 BSL

A

Agents are well classified and are not known to cause disease consistently in healthy adults

87
Q

Level 2 BSL

A

Agents that pose a moderation potential hazard for the employees & the environment

88
Q

Level 3 BSL

A

Agents that are indigenous or exotic, potential for aerosol transmission & may have serious lethal consequences

89
Q

Level 4 BSL

A 
Agents that are dangerous & exotic, high risk of life-threatening infections, aerosols, unknown risk of transmission
-PPE, BSC class III, neg pressure rooms, decontamination of room/personnel

Microbiology 484 (15 decks)

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