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Microbiology 484 > Gastrointestinal cultures > Flashcards

Gastrointestinal cultures Flashcards

1
Q

Organism as main growth after antibiotic treatment

A

Clostridium difficil

Screen with C serological screen

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2
Q

Fresh stool specimen handling

A

If can’t be plated within 1-2 hours, place in transport medium (cary-blair) for bacteria. Other transport for virus/parasites

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3
Q

Salmonella XLD reaction

A

Red colonies with black centers

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4
Q

Shigella XLD reactions

A

Colorless red colonies

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5
Q

Salmonella HE reaction

A

Green with black centers

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6
Q

Shigella HE reaction

A

Green

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7
Q

Salmonella Te7 reaction

A

Red

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8
Q

Shigella Te7 reaction

A

red

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9
Q

TSI Salmonella reaction

A

Red/yellow (K/A) with gas & H2S

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10
Q

TSI Shigella reaction

A

red/yellow (K/A)

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11
Q

LIA Salmonella reaction

A

Purple/Yellow (K/A) with H2S

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12
Q

LIA Shigella reaction

A

purple/yellow (K/A)

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13
Q

Urea salmonella reaction

A

Negative - yellow

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14
Q

Urea Shigella reaction

A

Negative - yellow

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15
Q

Salmonella infection

A

Ingestion of contaminated or undercooked poultry, beef, unpasteurized milk and eggs and person-to-person.
Causes gastroenteritis - usually self-limited up to serious epticemia

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16
Q

Shigella infection

A

Bloody stool, person to person contact

Usually self limiting

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17
Q

Campylobacter jejuni isolation media

A

Campy BAP/Brucella agar, 10% sheep blood with antibiotics

Environment at 42 and N 80-85%

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18
Q

Confirmation of Campylobacter jejuni

A

Curved gram neg rod, growth on Campy BAP, catalase +, nitrate +, sensitive to 30 ug disk of nalidixic acid, resistant to 30 ug disk of cephalothin

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19
Q

Campylobacter method of infection

A

Inadequately cooked poultry, untreated water, unpasteurized milk - exposure to animals with diarrhea (puppies & kittens)

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20
Q

Frequency of Campylobacter jejuni

A

Most common cause of diarrhea in US

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21
Q

Campylobacter jejuni course of illness

A

fever, pain, 1-7 day incubation but self-limiting

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22
Q

Yersinia enterocolitica media

A

CIN agar has sefsulodin, irgasan, novoiocin. Bile salts and crystal violet are inhibitory agents for normal colon organisms

23
Q

Yersinia enterocolitica colony morphology

A

“bulls eye” dark red or burgundy center surrounded by a translucent border
Oxidase =
Optimal growth 25-30

24
Q

Yersinia enterocolitica

source

A

Domestic animals, cats, dogs

25
Yersinia enterocolitica disease
acute enteritis, appendicitis-like syndrome, bloody stool
26
Vibrio cholera media
TCBS, which contains thiosulfate, citrate, bile salts, sucrose Temp: 35, curved neg rods
27
Vibrio cholera method of infection
Transferred through contaminated water, seafood
28
Vibrio cholera illness
Enterotoxin production is sever, C stimulates intestines to secrete water & electrolytes "Rice water stool"
29
enterotoxigenic E. coli
secrete enterotoxins, symptoms similar to Cholera | Traveler's diarrhea, diagnose by elimination of everything else
30
enterohemorrhagic E. coli
O157:H7 hemorrhagic diarrhea | Can be fatal among young & elderly
31
Enteropathogenic E. coli
Cause of infantile diarrhea
32
Enteroinvasive E. coli
Bloody diarrhea has invasive properties
33
Useful media for isolation of E. coli O157:O7
Sorbitol containing Mac plate - does not ferment sorbitol
34
E. coli O157:H7 symptoms
Hemorrhagic diarrhea, no pus in stool
35
E. coli O157:H7 method of infection
contaminated undercooked meat
36
E. coli O157:H7 course of disease
Low platelet count, hemolytic anemia, possible kidney failure
37
Aeromonas symptoms, method of infection, course of disease
Gastroenteritis usually from some aquatic exposure, untreated well water, raw oysters & clams -Self limiting
38
Aeromonas isolation
Straight negative rods, oxidase + on BAP, beta hemolysis
39
Staphylococcus aureus pathogen
Usually from infected food handler
40
Staphylococcus aureus symptoms
2-8 hours after ingestion of food
41
Staphylococcus aureus course of illness
no fever, but nausea, abdominal pain, severe cramping
42
Clostridium botulinum pathogen
ingestion of preformed toxins - very severe. From home-canning
43
Clostridium botulinum symptoms
Attacks neuromuscular junction of affected nerves
44
Clostridium botulinum symptoms
incubation time varies - need antitoxins
45
Clostridium perfringens pathogen
relatively mild - self limiting
46
Clostridium perfringens symptoms
8-12 hours incubation - resolves in 24 hours
47
Bacillus cereus pathogen
Ingestion of contaminated rice, poultry, meat
48
Bacillus cereus symptoms
Incubation 8-16 hours after ingestion, lasts 24 hours
49
Helicobacter pylori pathogen
Causative agent for most gastric ulcers - urease + - serologic tests for antibodies available
50
Upper small intestine NF
Strep, lacto bacilli, yeast
51
Lower intestine NF
Enterobacteriaceae, Enterococcus, Bacteroides, Clostridium peptostreptococcus, Bifidobacterium, Eubacterum Anaerobe: aerobes = 1000:1
52
Direct gram stain on stool specimens
Presence or absence of WBC, RBC, and mucus
53
Viral pathogens in gastrointestinal infections
Rotavirus, enteric adenovirus, calcivirus, astrovirus
54
Parasitic pathogens
Giardia lamblia, Entamoeba histolytica, Cryptosporidium parvum, Cyclospora caytenensis, microsporidia

Microbiology 484 (15 decks)

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