Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology 484 > Gastrointestinal cultures > Flashcards

Gastrointestinal cultures Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Organism as main growth after antibiotic treatment

A

Clostridium difficil

Screen with C serological screen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Fresh stool specimen handling

A

If can’t be plated within 1-2 hours, place in transport medium (cary-blair) for bacteria. Other transport for virus/parasites

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Salmonella XLD reaction

A

Red colonies with black centers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Shigella XLD reactions

A

Colorless red colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Salmonella HE reaction

A

Green with black centers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Shigella HE reaction

A

Green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Salmonella Te7 reaction

A

Red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Shigella Te7 reaction

A

red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

TSI Salmonella reaction

A

Red/yellow (K/A) with gas & H2S

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

TSI Shigella reaction

A

red/yellow (K/A)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

LIA Salmonella reaction

A

Purple/Yellow (K/A) with H2S

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

LIA Shigella reaction

A

purple/yellow (K/A)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Urea salmonella reaction

A

Negative - yellow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Urea Shigella reaction

A

Negative - yellow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Salmonella infection

A

Ingestion of contaminated or undercooked poultry, beef, unpasteurized milk and eggs and person-to-person.
Causes gastroenteritis - usually self-limited up to serious epticemia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Shigella infection

A

Bloody stool, person to person contact

Usually self limiting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Campylobacter jejuni isolation media

A

Campy BAP/Brucella agar, 10% sheep blood with antibiotics

Environment at 42 and N 80-85%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Confirmation of Campylobacter jejuni

A

Curved gram neg rod, growth on Campy BAP, catalase +, nitrate +, sensitive to 30 ug disk of nalidixic acid, resistant to 30 ug disk of cephalothin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Campylobacter method of infection

A

Inadequately cooked poultry, untreated water, unpasteurized milk - exposure to animals with diarrhea (puppies & kittens)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Frequency of Campylobacter jejuni

A

Most common cause of diarrhea in US

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Campylobacter jejuni course of illness

A

fever, pain, 1-7 day incubation but self-limiting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Yersinia enterocolitica media

A

CIN agar has sefsulodin, irgasan, novoiocin. Bile salts and crystal violet are inhibitory agents for normal colon organisms

23
Q

Yersinia enterocolitica colony morphology

A

“bulls eye” dark red or burgundy center surrounded by a translucent border
Oxidase =
Optimal growth 25-30

24
Q

Yersinia enterocolitica

source

A

Domestic animals, cats, dogs

25
Q

Yersinia enterocolitica disease

A

acute enteritis, appendicitis-like syndrome, bloody stool

26
Q

Vibrio cholera media

A

TCBS, which contains thiosulfate, citrate, bile salts, sucrose
Temp: 35, curved neg rods

27
Q

Vibrio cholera method of infection

A

Transferred through contaminated water, seafood

28
Q

Vibrio cholera illness

A

Enterotoxin production is sever, C stimulates intestines to secrete water & electrolytes
“Rice water stool”

29
Q

enterotoxigenic E. coli

A

secrete enterotoxins, symptoms similar to Cholera

Traveler’s diarrhea, diagnose by elimination of everything else

30
Q

enterohemorrhagic E. coli

A

O157:H7 hemorrhagic diarrhea

Can be fatal among young & elderly

31
Q

Enteropathogenic E. coli

A

Cause of infantile diarrhea

32
Q

Enteroinvasive E. coli

A

Bloody diarrhea has invasive properties

33
Q

Useful media for isolation of E. coli O157:O7

A

Sorbitol containing Mac plate - does not ferment sorbitol

34
Q

E. coli O157:H7 symptoms

A

Hemorrhagic diarrhea, no pus in stool

35
Q

E. coli O157:H7 method of infection

A

contaminated undercooked meat

36
Q

E. coli O157:H7 course of disease

A

Low platelet count, hemolytic anemia, possible kidney failure

37
Q

Aeromonas symptoms, method of infection, course of disease

A

Gastroenteritis usually from some aquatic exposure, untreated well water, raw oysters & clams
-Self limiting

38
Q

Aeromonas isolation

A

Straight negative rods, oxidase + on BAP, beta hemolysis

39
Q

Staphylococcus aureus pathogen

A

Usually from infected food handler

40
Q

Staphylococcus aureus symptoms

A

2-8 hours after ingestion of food

41
Q

Staphylococcus aureus course of illness

A

no fever, but nausea, abdominal pain, severe cramping

42
Q

Clostridium botulinum pathogen

A

ingestion of preformed toxins - very severe. From home-canning

43
Q

Clostridium botulinum symptoms

A

Attacks neuromuscular junction of affected nerves

44
Q

Clostridium botulinum symptoms

A

incubation time varies - need antitoxins

45
Q

Clostridium perfringens pathogen

A

relatively mild - self limiting

46
Q

Clostridium perfringens symptoms

A

8-12 hours incubation - resolves in 24 hours

47
Q

Bacillus cereus pathogen

A

Ingestion of contaminated rice, poultry, meat

48
Q

Bacillus cereus symptoms

A

Incubation 8-16 hours after ingestion, lasts 24 hours

49
Q

Helicobacter pylori pathogen

A

Causative agent for most gastric ulcers

  • urease +
  • serologic tests for antibodies available
50
Q

Upper small intestine NF

A

Strep, lacto bacilli, yeast

51
Q

Lower intestine NF

A

Enterobacteriaceae, Enterococcus, Bacteroides, Clostridium peptostreptococcus, Bifidobacterium, Eubacterum

Anaerobe: aerobes = 1000:1

52
Q

Direct gram stain on stool specimens

A

Presence or absence of WBC, RBC, and mucus

53
Q

Viral pathogens in gastrointestinal infections

A

Rotavirus, enteric adenovirus, calcivirus, astrovirus

54
Q

Parasitic pathogens

A

Giardia lamblia, Entamoeba histolytica, Cryptosporidium parvum, Cyclospora caytenensis, microsporidia

Microbiology 484 (15 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions