Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology 484 > Nonfermenters/Unusual Neg Rods/ Haemophilus > Flashcards

Nonfermenters/Unusual Neg Rods/ Haemophilus Flashcards

1
Q

Pseudomonas
Acinetobacter
Flavoacteriaceae

A

Nonfermenters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q 
Stenotrophomonas
Alcaligenes + Achromobacter
Methylobacterium 
Shewanella
Moraxella + Oligella
A

Nonfermenters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Burkolderia
Shigomonas
Chromobacterium

A

Nonfermenters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Baleatrix
Brevundimonas
Comanomas

A

Nonfermenters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

3 clues that indicate nonfermenter

A
  • Clear colonies on the MAC plate
  • Poor growth in automated in 24 hour
  • Oxidase pos reactions (some are neg)
  • Coccobacilli or very long gram = bacilli
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Typical TSI reaction for a nonfermenters

A

K/K

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Fermentative (OF test)

A

Will utilize glucose in an open or closed system (fermentor)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Oxidative (OF test)

A

Will utilize glucose only in open tube (non fermentor)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Nonsaccharolytic (OF test)

A

Will not utilize the sugar in either system

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Pycocyanin

A

A water soluble pigment produced by P. aeruginosa that gives a blue-green appearance with a slightly metallic surface

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Pyoverdin

A

Water soluble fluorescent pigment that is produced by P. flourescens & P. putida & P. aeruginosa

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Pseudomonas aeruginosa pathogenicity

A 
Wound infections
Nosocomial
Swimmers ear
Pulmonary disease
Increased in CF patients
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Pseudomonas aeruginosa special identifying features

A 
Very resistant to antibiotics
Green sheen
Ox pos
Grape/corn chip-like odor
Large flat spready colony
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Pseudomonas aeruginosa growth requirements

A

Will tolerate 42 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Pseudomonas fluorescens/putida pathogenicity

A

Low virulence

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Pseudomonas fluorescens/putida special ID features

A

Produce pyoverdine

Ox pos

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Pseudomonas fluorescens/putida growth requirements

A

37 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Stenotrophomonus maltophilia pathogenicity

A

Nosocomial infections in contaminated equip (#1 venous cath)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Stenotrophomonus maltophilia special ID features

A

Ox neg

Non fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Stenotrophomonus maltophilia growth requirements

A

37 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Acinetobacter baumannii/ lwoffi pathogenicity

A

Opportunistic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Acinetobacter baumannii/ lwoffi special ID features

A

Ox neg
Coccobacilli
Cat +
Nonmotile

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Acinetobacter baumannii/ lwoffi growth requirements

A

Purple colony on MAC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Burkholderia cepacia pathogenicity

A

Nosocomial infections

Increased in CF patients

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Burkholderia cepacia special ID features
Slow ox pos results
26
Burkholderia cepacia growth requirements
Can grow on MAC & blood but is inhibited by NF | Grows best on OFPBL
27
Flacobacteriaceae pathogenicity
Nosocomial
28
Flacobacteriaceae special ID features
Weak fermenters so appear as nonfermenters | Ox pos
29
Flacobacteriaceae special iso procedures
Yellow pigment
30
Moraxella pathogenicity
Opportunists
31
Moraxella special ID features
Ox pos Nonmotile Coccobacilli Don't utilize common carbs
32
Oligella pathogenicity
Opportunists
33
Oligella special ID features
Coccobacilli
34
Oligella growth requirements
No growth on MAC
35
Colony morphology of Psuedomonas stutzeri
Wrinkled, leathery colony with a light yellow to brown pigment
36
Significant characteristics of Burkholderia cepacia
``` Nosocomial pathogen Attacks cystic fibrosis patients frequently Prefers 30 C Oxidase = Yellow pigment - non-wrinkled colony ```
37
Burkholeria pseudomallei pathogenicity
Causes meliodiosis granulomatous pulmonary disease
38
Burkholeria pseudomallei characteristics
Has bipolar staining on gram stains, nonfermenter with a wrinkled colony "Earthy odor"
39
Burkholeria mallei pathogenicity
Bioterroristic organism | Causes glanders
40
Burkholeria mallei characteristics
Nonmotile | Gram neg coccobacilli that may branch & look filamentous
41
Haemophilus phenotypic characteristics
Require X and/or V factors
42
Enterobacteriaceae phenotypic characteristics
All are oxidase neg | Change nitrates --> nitrites & ferment glucose
43
Non fermenters phenotypic characteristics
Don't ferment glucose
44
HACEK Group
``` H = Haemophilus spp (new name Aggregatibacter Aphrophilus) A = Actinobacillus actinomycetemcomitans C = Cardiobacterium hominis E = Eikenella carrodens K = Kingella ```
45
HACEK Group characteristics
All need increased CO2 & their love of heart valves | All NF except Aphrophilus
46
Actinobacillus pathogenicity
Zoonotic | Isolated from blood
47
Actinobacillus special ID features
Nonmotile Coccobacilli 48 hr - star like colonies
48
Actinobacillus growth
increased CO2 | Mac neg
49
Cardiobacterium pathogenicity
Respiratory & GI normal flora | May cause endocarditis following dental visits
50
Cardiobacterium Special ID features
Pleomorphic neg rod Hemolytic Pits agar Nonmotile
51
Cardiobacterium growth
Increased CO2 | Mac negative
52
Eikenella pathogenicity
Respiratory & GI NF | -Human teeth bites
53
Eikenella Special ID features
Coccobacilli Ox pos Yellow pigment Pits agar/bleach odor
54
Eikenella growth requirements
Increased CO2 | Poor on Mac
55
Kingella pathogenicity
Rare pathogen
56
Kingella Special ID features
Pairs or short chained coccobacilli | Ox pos
57
Kingella special growth
Increased CO2 | 42 C
58
Haemophilus general characteristics
Gram neg coccobacillus Ox & catalase pos Like increased CO2
59
H. influenzae location on body
Upper respiratory
60
H. aegytpius location on body
Eye
61
H. ducreyi location on body
Genital
62
H. influenzae X & V requirements
Requires both X & V
63
H. aegyptius X & V requirements
requires both X & V
64
H. ducreyi X & V requirements
Requires X only, | Ox =
65
Hemophilic
Blood lover
66
X factor
Hemin
67
V factor
NADC (nicotinamide-adenine dinucleotide)
68
Porphyrin Test
Looks for the organism's ability to convert ALA to porphyrin. Takes place of the X test. If org requires X to be provided, porphyrin =
69
Satellitism
If Haemophilus requires both X & V factor, on BAP the X would be available from media, V would be donated from Strep pneumoniae, Staph aureus, Neisseria Grows around these colonies as tiny, gray shiny colonies
70
H. influenzae capsule
When antigens are from for the 6 serotypes (a, b, c, d, e, f) B most invasive
71
H. influenzae IgA
Protease lyses IgA's so weakens defenses in mucous membranes
72
H. influenzae adherence
Lack of adherence may play a role in the orgs ability to become systemic
73
H. influenzae LPS
paralyzing effect of the ciliated respiratory epithelium
74
Pathogenicity of H. influenzae serotype B
Affects children between ages 3 months to 6 years, | Causative agent of meningitis, cellulitis, epiglottitis
75
Antibiotics used in life-threatening H. influenzae infections
Cefotaxime or ceftriazone alternates (tremethoprim, SXT, imipenam, ciprofloxacin)
76
Antibiotics used in non life-threatening H. influenzae infections
Amoxicillin or 2 or 3rd gen cephalosporins
77
Antibiotics used in H. ducreyi infections
Erythromycin
78
Mechanism for testing for resistance to ampicillin
A disk containing Cefinase is rehydrated with water. Several colones are placed on disk. If the org has the enzyme, it breaks the B-lactam ring of the cefinase & color reaction occurs
79
Pasterurella multocida pathogenicity
Cellulitis from cat scratch or bites
80
Pasterurella multocida special ID features
Pleomorphic gram = rod
81
Pasterurella multocida growth requirements
Grow on BAP ox + Cat +
82
Brucella pathogenicity
Zoonotic - infects through contact with infected animals & animal products
83
Brucella special ID features
Multiple blood cultures held for 30 days
84
Brucella special isolation procedures
serological testing most beneficial
85
Legionella pathogenicity
Pneumonia Legionnaires disease Pontiac fever
86
Legionella special ID features
Urine antigen test Fl testing DNA probe
87
Legionella growth requirements
20-43 C BCYE isolation Ground glass appearance
88
Francisella pathogenicity
Tuleremia | Zoonotic - usually infected by rabbits or ticks
89
Francisella special ID features
Ulcer specimens or biopsy of lymph nodes
90
Francisella special iso procedures
Serological studies
91
Bordetella pertussis pathogenicity
Whooping cough or pertussis
92
Bordetella pertussis special ID features
Direct Fl testing | DNA probes
93
Bordetella pertussis growth requirements
Regan-Lowe | Bordet-Gengou media
94
Gardnerella pathogenicity
bacterial vaginosis
95
Gardnerella special ID features
epithelial cell with borders obscured by tiny gram = rods (clue cells)
96
Gardnerella special iso procedures
B hemolytic on V agar
97
Campylobacter pathogenicity
Most common cause of bacterial gastroenteritis
98
Campylobacter special ID features
Darting motility Nonfermenter curved gram strain Ox +, cat +
99
Campylobacter growth requirements
Campy BAP | 42 C with 85% N2
100
Helicobacter pathogenicity
Gastric & duodenal ulcers
101
Helicobacter special ID features
Rapidly reacting urease
102
Helicobacter special iso procedures
Serological testing available
103
Vibrio cholerae pathogenicity
Causative agent of a severe life threatening diarrheal disease
104
Vibrio cholerae ID
Curved gram neg rods but straight on lab growth | Ox +, nitrates --> nitrites, halophilic, + string test, susceptible to O/129
105
Vibrio cholerae growth requirements
Require addition of Na+ | TCBS media
106
Aeromonas pathogenicity
Causes gastroenteritis, wound infections, septicemia | Distributed in water worldwide
107
Aeromonas ID
Ox + on blood agar plate, glucose fermenting
108
Plesiomonas pathogenicity
Causes gastroenteritis Found in soil & water Has been moved to Enterobacteriaceae family
109
Plesiomonas ID
Ox +, glucose fermenting | Looks like E. coli on regular enteric media
110
Plesiomonas growth requirements
Cannot grow at less than 8 C

Microbiology 484 (15 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions