Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology 484 > Nonfermenters/Unusual Neg Rods/ Haemophilus > Flashcards

Nonfermenters/Unusual Neg Rods/ Haemophilus Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Pseudomonas
Acinetobacter
Flavoacteriaceae

A

Nonfermenters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q 
Stenotrophomonas
Alcaligenes + Achromobacter
Methylobacterium 
Shewanella
Moraxella + Oligella
A

Nonfermenters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Burkolderia
Shigomonas
Chromobacterium

A

Nonfermenters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Baleatrix
Brevundimonas
Comanomas

A

Nonfermenters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

3 clues that indicate nonfermenter

A
  • Clear colonies on the MAC plate
  • Poor growth in automated in 24 hour
  • Oxidase pos reactions (some are neg)
  • Coccobacilli or very long gram = bacilli
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Typical TSI reaction for a nonfermenters

A

K/K

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Fermentative (OF test)

A

Will utilize glucose in an open or closed system (fermentor)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Oxidative (OF test)

A

Will utilize glucose only in open tube (non fermentor)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Nonsaccharolytic (OF test)

A

Will not utilize the sugar in either system

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Pycocyanin

A

A water soluble pigment produced by P. aeruginosa that gives a blue-green appearance with a slightly metallic surface

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Pyoverdin

A

Water soluble fluorescent pigment that is produced by P. flourescens & P. putida & P. aeruginosa

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Pseudomonas aeruginosa pathogenicity

A 
Wound infections
Nosocomial
Swimmers ear
Pulmonary disease
Increased in CF patients
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Pseudomonas aeruginosa special identifying features

A 
Very resistant to antibiotics
Green sheen
Ox pos
Grape/corn chip-like odor
Large flat spready colony
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Pseudomonas aeruginosa growth requirements

A

Will tolerate 42 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Pseudomonas fluorescens/putida pathogenicity

A

Low virulence

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Pseudomonas fluorescens/putida special ID features

A

Produce pyoverdine

Ox pos

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Pseudomonas fluorescens/putida growth requirements

A

37 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Stenotrophomonus maltophilia pathogenicity

A

Nosocomial infections in contaminated equip (#1 venous cath)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Stenotrophomonus maltophilia special ID features

A

Ox neg

Non fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Stenotrophomonus maltophilia growth requirements

A

37 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Acinetobacter baumannii/ lwoffi pathogenicity

A

Opportunistic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Acinetobacter baumannii/ lwoffi special ID features

A

Ox neg
Coccobacilli
Cat +
Nonmotile

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Acinetobacter baumannii/ lwoffi growth requirements

A

Purple colony on MAC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Burkholderia cepacia pathogenicity

A

Nosocomial infections

Increased in CF patients

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Burkholderia cepacia special ID features

A

Slow ox pos results

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Burkholderia cepacia growth requirements

A

Can grow on MAC & blood but is inhibited by NF

Grows best on OFPBL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Flacobacteriaceae pathogenicity

A

Nosocomial

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

Flacobacteriaceae special ID features

A

Weak fermenters so appear as nonfermenters

Ox pos

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

Flacobacteriaceae special iso procedures

A

Yellow pigment

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Moraxella pathogenicity

A

Opportunists

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Moraxella special ID features

A

Ox pos
Nonmotile
Coccobacilli
Don’t utilize common carbs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

Oligella pathogenicity

A

Opportunists

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

Oligella special ID features

A

Coccobacilli

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

Oligella growth requirements

A

No growth on MAC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

Colony morphology of Psuedomonas stutzeri

A

Wrinkled, leathery colony with a light yellow to brown pigment

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

Significant characteristics of Burkholderia cepacia

A 
Nosocomial pathogen
Attacks cystic fibrosis patients frequently 
Prefers 30 C
Oxidase = 
Yellow pigment - non-wrinkled colony
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

Burkholeria pseudomallei pathogenicity

A

Causes meliodiosis granulomatous pulmonary disease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

Burkholeria pseudomallei characteristics

A

Has bipolar staining on gram stains, nonfermenter with a wrinkled colony
“Earthy odor”

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

Burkholeria mallei pathogenicity

A

Bioterroristic organism

Causes glanders

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

Burkholeria mallei characteristics

A

Nonmotile

Gram neg coccobacilli that may branch & look filamentous

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

Haemophilus phenotypic characteristics

A

Require X and/or V factors

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
42
Q

Enterobacteriaceae phenotypic characteristics

A

All are oxidase neg

Change nitrates –> nitrites & ferment glucose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
43
Q

Non fermenters phenotypic characteristics

A

Don’t ferment glucose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
44
Q

HACEK Group

A 
H = Haemophilus spp (new name Aggregatibacter Aphrophilus)
A = Actinobacillus actinomycetemcomitans
C = Cardiobacterium hominis
E = Eikenella carrodens
K = Kingella
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
45
Q

HACEK Group characteristics

A

All need increased CO2 & their love of heart valves

All NF except Aphrophilus

46
Q

Actinobacillus pathogenicity

A

Zoonotic

Isolated from blood

47
Q

Actinobacillus special ID features

A

Nonmotile
Coccobacilli
48 hr - star like colonies

48
Q

Actinobacillus growth

A

increased CO2

Mac neg

49
Q

Cardiobacterium pathogenicity

A

Respiratory & GI normal flora

May cause endocarditis following dental visits

50
Q

Cardiobacterium Special ID features

A

Pleomorphic neg rod
Hemolytic
Pits agar
Nonmotile

51
Q

Cardiobacterium growth

A

Increased CO2

Mac negative

52
Q

Eikenella pathogenicity

A

Respiratory & GI NF

-Human teeth bites

53
Q

Eikenella Special ID features

A

Coccobacilli
Ox pos
Yellow pigment
Pits agar/bleach odor

54
Q

Eikenella growth requirements

A

Increased CO2

Poor on Mac

55
Q

Kingella pathogenicity

A

Rare pathogen

56
Q

Kingella Special ID features

A

Pairs or short chained coccobacilli

Ox pos

57
Q

Kingella special growth

A

Increased CO2

42 C

58
Q

Haemophilus general characteristics

A

Gram neg coccobacillus
Ox & catalase pos
Like increased CO2

59
Q

H. influenzae location on body

A

Upper respiratory

60
Q

H. aegytpius location on body

A

Eye

61
Q

H. ducreyi location on body

A

Genital

62
Q

H. influenzae X & V requirements

A

Requires both X & V

63
Q

H. aegyptius X & V requirements

A

requires both X & V

64
Q

H. ducreyi X & V requirements

A

Requires X only,

Ox =

65
Q

Hemophilic

A

Blood lover

66
Q

X factor

A

Hemin

67
Q

V factor

A

NADC (nicotinamide-adenine dinucleotide)

68
Q

Porphyrin Test

A

Looks for the organism’s ability to convert ALA to porphyrin. Takes place of the X test. If org requires X to be provided, porphyrin =

69
Q

Satellitism

A

If Haemophilus requires both X & V factor, on BAP the X would be available from media, V would be donated from Strep pneumoniae, Staph aureus, Neisseria
Grows around these colonies as tiny, gray shiny colonies

70
Q

H. influenzae capsule

A

When antigens are from for the 6 serotypes (a, b, c, d, e, f)
B most invasive

71
Q

H. influenzae IgA

A

Protease lyses IgA’s so weakens defenses in mucous membranes

72
Q

H. influenzae adherence

A

Lack of adherence may play a role in the orgs ability to become systemic

73
Q

H. influenzae LPS

A

paralyzing effect of the ciliated respiratory epithelium

74
Q

Pathogenicity of H. influenzae serotype B

A

Affects children between ages 3 months to 6 years,

Causative agent of meningitis, cellulitis, epiglottitis

75
Q

Antibiotics used in life-threatening H. influenzae infections

A

Cefotaxime or ceftriazone alternates (tremethoprim, SXT, imipenam, ciprofloxacin)

76
Q

Antibiotics used in non life-threatening H. influenzae infections

A

Amoxicillin or 2 or 3rd gen cephalosporins

77
Q

Antibiotics used in H. ducreyi infections

A

Erythromycin

78
Q

Mechanism for testing for resistance to ampicillin

A

A disk containing Cefinase is rehydrated with water. Several colones are placed on disk. If the org has the enzyme, it breaks the B-lactam ring of the cefinase & color reaction occurs

79
Q

Pasterurella multocida pathogenicity

A

Cellulitis from cat scratch or bites

80
Q

Pasterurella multocida special ID features

A

Pleomorphic gram = rod

81
Q

Pasterurella multocida growth requirements

A

Grow on BAP
ox +
Cat +

82
Q

Brucella pathogenicity

A

Zoonotic - infects through contact with infected animals & animal products

83
Q

Brucella special ID features

A

Multiple blood cultures held for 30 days

84
Q

Brucella special isolation procedures

A

serological testing most beneficial

85
Q

Legionella pathogenicity

A

Pneumonia
Legionnaires disease
Pontiac fever

86
Q

Legionella special ID features

A

Urine antigen test
Fl testing
DNA probe

87
Q

Legionella growth requirements

A

20-43 C
BCYE isolation
Ground glass appearance

88
Q

Francisella pathogenicity

A

Tuleremia

Zoonotic - usually infected by rabbits or ticks

89
Q

Francisella special ID features

A

Ulcer specimens or biopsy of lymph nodes

90
Q

Francisella special iso procedures

A

Serological studies

91
Q

Bordetella pertussis pathogenicity

A

Whooping cough or pertussis

92
Q

Bordetella pertussis special ID features

A

Direct Fl testing

DNA probes

93
Q

Bordetella pertussis growth requirements

A

Regan-Lowe

Bordet-Gengou media

94
Q

Gardnerella pathogenicity

A

bacterial vaginosis

95
Q

Gardnerella special ID features

A

epithelial cell with borders obscured by tiny gram = rods (clue cells)

96
Q

Gardnerella special iso procedures

A

B hemolytic on V agar

97
Q

Campylobacter pathogenicity

A

Most common cause of bacterial gastroenteritis

98
Q

Campylobacter special ID features

A

Darting motility
Nonfermenter
curved gram strain
Ox +, cat +

99
Q

Campylobacter growth requirements

A

Campy BAP

42 C with 85% N2

100
Q

Helicobacter pathogenicity

A

Gastric & duodenal ulcers

101
Q

Helicobacter special ID features

A

Rapidly reacting urease

102
Q

Helicobacter special iso procedures

A

Serological testing available

103
Q

Vibrio cholerae pathogenicity

A

Causative agent of a severe life threatening diarrheal disease

104
Q

Vibrio cholerae ID

A

Curved gram neg rods but straight on lab growth

Ox +, nitrates –> nitrites, halophilic, + string test, susceptible to O/129

105
Q

Vibrio cholerae growth requirements

A

Require addition of Na+

TCBS media

106
Q

Aeromonas pathogenicity

A

Causes gastroenteritis, wound infections, septicemia

Distributed in water worldwide

107
Q

Aeromonas ID

A

Ox + on blood agar plate, glucose fermenting

108
Q

Plesiomonas pathogenicity

A

Causes gastroenteritis
Found in soil & water
Has been moved to Enterobacteriaceae family

109
Q

Plesiomonas ID

A

Ox +, glucose fermenting

Looks like E. coli on regular enteric media

110
Q

Plesiomonas growth requirements

A

Cannot grow at less than 8 C

Microbiology 484 (15 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions