Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology 484 > Enterobacteriaceae > Flashcards

Enterobacteriaceae Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Shigella motility

A

100% neg

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Kleb pneumo motility

A

100% neg

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Proteus spp phenylalanine deaminase

A

positive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Providencia phenylalanine deaminase

A

positive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Principle of serological typing of Salmonella & Shigella species

A

Somatic O antigens & flagella H antigens for Salmonella

O antigens for Shigella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Escherichieae tribe

A

Escherichia, Shigella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Edwardsielleae tribe

A

Edwardsiella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Salmonelleae tribe

A

Salmonella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Citrobacteriaceae tribe

A

Citrobacter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Klebsielleae tribe

A

Klebsiella, Enterobacter, Hafnia, Serratia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Proteae tribe

A

Proteus, Morganella, Providencia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Yersinieae tribe

A

Yersinia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

IMVIC reactions for E. coli

A

Indole +
MR +
VP =
Citrate +

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

IMVIC reactions for Klebsiella pneumoniae

A

Indole =
MR =
VP +
Citrate +

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

IMVIC reactions for Klebsiella oxytoca

A

Indole +
MR =
MP +
Citrate +

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

IMVIC reactions for Enterobacter aerogenes

A

Indole =
MR =
VP +
Citrate +

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

IMVIC reactions for Enterobacter intermedius

A

Indole =
MR +
VP +
Citrate +

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

4 characteristics shared by members of Enterobacteriacea

A

Gram neg rod/bacilli
Oxidase =
Convert nitrate to nitrites
Ferment glucose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

K antigen of Enterobacteriaceae

A

Capsular antigen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

O antigen of Enterobacteriaceae

A

somatic antigen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

H antigen of Enterobacteriaceae

A

Flagellar antigen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

TSI

-K/A

A

Utilizes glucose only

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

TSI

-K/K

A

Non fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

TSI

-A/A

A

Utilize glucose and/or lactose and/or sucrose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

TSI

-No gas production

A

Nonaerogenic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

TSI

Gas production

A

Aerogenic, H2S production

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

LIA

-K/A

A

Utilize glucose only

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

LIA

-K/K

A

Decarboxylated lysine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

LIA

-R/Y

A

Phenylalanine deaminase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Indole

A

If organism has enzyme tryptophanase, it will deaminate tryptophan & make product indole

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Methyl red

A

Glucose is metabolized by mixed acid fermentation & produces an acidic end product, which decreases the pH & gives a color change

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

Voges-Proskauer

A

Acids formed are metabolized to 2,3-butanediol through acetoin, alpha napthol & 40% KOH are added, resulting in a red complex

33
Q

Citrate

A

Uses citrate as sole source of carbon

34
Q

Urea

A

If organism an hydrolyze urea to ammonia

35
Q

Amino acid degradation

-Lysine

A

–> cadaverine

36
Q

Amino acid degradation

-Arginine

A

–> putrescine (production of ammonia causes color change)

37
Q

Amino acid degradation

-Ornithine

A

–> putrescine (production of ammonia causes color change)

38
Q

ONPG

A

If organism ahs B-galactosidase, it acts on ONPG to produce galactose & orthonitrophenol - a neg reaction will occur if the org is a non-lactose fermenter

39
Q

PYR

A

The organism can hydrolyze the substrate PYR

  • L-prolidonyl-B naphthylamide
  • L-pyroglutamic acid-B naphthylamide
40
Q

Hippurate

A

If organism has enzyme hippicurase, will hydrolyze sodium hippurate

41
Q

Hydrogen sulfide

A

Organism’s ability to produce H2S

42
Q

Tryptophan deaminase (TDA)

A

Produces indole pyruvic acid which reacts with ferric ammonium citrate in the medium after addition of ferric chloride to produce a brown color

43
Q

Nitrate

A

Conversion of nitrate to nitrite

44
Q

Esculin

A

If organism hydrolyzes esculin, the product will react with ferric salt to form a black precipitate

45
Q

Why suspected Salmonella species isolates boiled for 20 if no reaction with O typing sera

A

Vi antigen may block the O antigen & may give a false negative.
If Vi antigen is heated, it will be removed & allow access to the O antigen

46
Q

Sorbitol MacConkey agar isolation pathogen

A

E.coli O157:H7
Causes hemorrhagic diarrhea & hemolytic uremic syndrome.
Appears as a clear/colorless colony on SMAC

47
Q

Indole positive Proteus

A

Proteus vulgaris

48
Q

3 sources Salmonella typhi can be isolated from during typhoid fever

A

Blood, gallbladder, GI tract

49
Q

Carrier state

A

Carriers shed organisms continuously or intermittently & feces may contaminate food or water sources

50
Q

Treatment of Salmonella gastroenteritis

A

If treated by antibiotics, patient may have a higher risk for developing carrier state
If treatment is necessary, use trimethoprim-sulfamethoxazole

51
Q

Purpose of MacConkey & eosin Methylene Blue agar

A

Selective: isolate gram neg rods, inhibit gram pos
differential: lactose positive & negative

52
Q

E. coli on EMB agar

A

Forms blue-black colonies with metallic-greenish sheen

53
Q

GN broth purpose

A

Selective enrichment media that inhibits gram pos bacteria & has high % of mannitol, which temporarily favors mannitol-fermenting gram neg rods

54
Q

Zinc dust use in nitrate test

A

If neg color reaction, zinc dust added to the nitrate test will show a color if nitrate is still present, which shows a true negative
If true neg color reaction, the nitrate has been reduced to nitrite then further reduced to gaseous products or a true pos reaction

55
Q

Non-lactose & lactose fermenters on EMB

A

Non-lactose: translucent, amber color

Lactose: pink

56
Q

Non-lactose & lactose fermenters on MAC

A

Non-lactose fermenters: colorless of transparent

Lactose: pink/red

57
Q

Non-lactose & lactose fermenters on HE

A

non-lactose: green/blue green. black H2s

Lactose: orange/salmon-pink

58
Q

Non-lactose & lactose fermenters on XLD

A

Non-lactose: red/colorless. black H2S

Lactose: yellow

59
Q

Non-lactose & lactose fermenters on TERGITOL-7

A

Non-lactose: red

lactose: green/blue

60
Q

Coliforms

A

Organisms commonly seen in GI tract (neg rods) as normal for a
-E. coli, Klebsiella, Enterobacter, Proteus, Pseudomonas

61
Q

Tribe with strong positive urea reaction

A

Proteeae tribe

62
Q

Vitek system

A

Inoculum size is small & is an enclosed walk-away system

63
Q

Microscan system

A

Fluorescent color change. If the microbe begins to se the substance in the well, it breaks a quenching bond for fl. and the well will fluoresce.

64
Q

Klebsiella pneumoniae reactions

A 
TSI: K/A + G
MIO: ===
CIT: +
LIA: K/K 
UREA +
65
Q

Klebsiella oxytoca

A 
TSI: K/A + G
MIO: =+=
CIT +
LIA: K/K
Urea: +
66
Q

Enterobacter aerogenes

A 
TSI: A/A +G
MIO: +=+
CIT: +
LIA: K/K
Urea: =
67
Q

E. coli

A 
TSI: K or A/A +G
MIO: +++
CIT: =
LIA: K/K or A
Urea: =
68
Q

Salmonella

A 
TSI: K/A +H2S
MIO: +=+
CIT: = 
LIA: K/K +H2S
Urea: =
69
Q

Citrobacter fruendii

A 
TSI: K/A +H2S
MIO: +==
CIT: +
LIA: K/A +H2S
Urea: +
70
Q

Proteus vulgaris

A 
TSI: A/A +G +H2S
MIO: ++=
CIT: =
LIA: R/A
Urea: +
71
Q

Proteus mirabilis

A 
TSI: K/A +G +H2S
MIO: +=+
CIT: +
LIA: R/A
Urea: +
72
Q

Shigella

A 
TSI: K/A
MIO: ===
CIT: = 
LIA: K/A
Urea: =
73
Q

Chemicals used in the oxidase test

A

C tetra methyl-p-phynylene-diamine-dihydrochloride

74
Q

Chemicals used in the indole test

A

C paradimethylamnobenzaldehyde

75
Q

Kleb pneumoniae colony morphology

A

pink mucoid colonies

76
Q

Proteus morphology

A

swarm on non-selective media

77
Q

E. coli morphology

A

Usually dryer pink with surrounding pink precipitated bile salt

78
Q

Yersinia optimal temperature and atmosphere

A

Grows best at 25-30 degrees Celcuis, ambient atmosphere, but will also grow at 37 degree

Microbiology 484 (15 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions