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Microbiology 484 > Respiratory Cultures > Flashcards

Respiratory Cultures Flashcards

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Biology 101 flashcards
1
Q

NF of upper respiratory tract

A

Strep mitis & other alpha strep, Staph aureus, Beta strep (not group A), H. influenzae, Strep pneumoniae, Moraxella catarrhalis, coag neg staph, Candida, Neisseria spp, non-hemolytic strep, Veillonella, bacteroides

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2
Q

Common cause of epiglottis

A

Haemophilus influenzae type B

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3
Q

Major concern in cystic fibrosis patients

A

Haemophilus influenzae, Staph aureus, Pseudomonas aeruginosa, Stenotrophomonas, Alcaligenes

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4
Q

Specimen collected for Bordetella pertussis

A

Nasopharyngeal swab using a calcium alginate swab

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5
Q

Media used for Bordetella pertussis

A

Boret-Gengou media, Regan-Low, modified Jones-Kendrick charcoal
Fl stain available, but if neg, must do culture

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6
Q

Cause of Vincent stomatitis or trench mouth

A

Severe cause of gingivitis. Caused by combined effect of anaerobes (Fusobacterium necophorum)

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7
Q

Purpose of PMN in sputum

A

PMNs suggest true sputum

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8
Q

Purpose of squamous epithelial cells in sputum

A

suggest only saliva & inadequate specimen

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9
Q

Significant findings on sputum gram stain

A

A disproportionate amount of any single type of bacterial morphology may be significant
Ex. gnr, gn diplococcic, gp diplococcic, or lack of bacteria

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10
Q

Organism that causes lobar pneumonia in a child

A

Strep pneumonia and H. influenzea type B

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11
Q

Organism that causes atypical pneumonia

A

Mycoplasma penumoniae

Cannot grow on culture - must Id by molecular amplification

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12
Q

Reason for SBA for isolation of beta hemolytic strep

A

Only blood agar that demonstrates valid reaction for hemolysis

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13
Q

What organism would be suspected from this sputum gram stain?

3+ gn diplococci
1+ gpc chains
1+ gp rods diptheroids
>25 PMNS
<10 epi
A

Moraxella catarrhalis

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14
Q

What organism would be suspected from this sputum gram stain?

4+ gpc chains &amp; clusters
4+ gp rods
1+ gnr
<25 PMN
>10 epi
A

Saliva contamination, not acceptable specimen for culture

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15
Q

What organism would be suspected from this sputum gram stain?

4+ gp diplococci
2+ neg diplococci
>25 PMN
<10 epi

A

Strep pneumoniae

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16
Q

What organism would be suspected from this sputum gram stain?

3+ tiny gnr
2+ gpc
>25 PMN
<10 epi

A

Haemophilus influenzae

17
Q

Moraxella catarrhalis biochemical tests

A

Oxidase +
glucose, maltose, sucrose, lactose =
DNase +
Tributryn +

18
Q

Pathogenicity of Moraxella catarrhalis

A

Cause lower lobe pneumoniae

19
Q

Screening for Group A beta Strep

A

Beta hemolysis around tiny pinpoint colony, confirm by antigen testing, bacitracin, PYR

20
Q

What are you screening for on the choc plate in sputum culture

A

H. influenzae

21
Q

What are you screening for on the blood agar plate in a sputum culture?

A

alpha hemolysis for Strep pneumonia, excess amount of Staph aureus, excess amount of yeast, hemolysis for group A beta Strep, presence or absence of normal flora

22
Q

What are you screening for on the Mac plate in sputum culture?

A

Excess gram neg rod. Klebsiella pneumoniae

23
Q

What must be done if the EIA screen for group A beta strep is neg

A

do culture

24
Q

When will H. influenza grow on a blood agar plate?

A

If another organism gives it V factor. X factor is coming from the plate (satellitism)

25
Q

Best specimen for culture of Bordetella pertussis and N. meningitides?

A

Nasopharyngeal swabs

26
Q

Respiratory symptoms of C. diphtheriae

A

Pharyngitis that causes a membrane to be produced & covers tonsils, palate, pharyngeal wall, causing respiratory obstruction. toxin release is also problem

27
Q

Cutaneous symptoms of C. diphtheriae

A

Membrane formation in non healing ulcers

28
Q

ID of C. diphtheriae

A

Shows a halo on tinsdales agar & will hydrolyze urea.

Definitive ID must be done by demonstration of toxin

29
Q

Importance of identifying capsulated H. influenza in respiratory cultures

A

may be associated with meningitis, epiglottitis, cellulitis, bacteremia, septic arthritis, pneumonia

30
Q

Importance in ID of non-encapsulated H. influenza in respiratory cultures

A

Cause otitis media, sinusitis, conjunctivitis, chronic bronchitis, pneumonia, & bacteremia in adults with chronic disease

31
Q

Community acquired pneumonia in children

A

Most common: RSV, influenza, A/B, para influenza, 1/2/3 adenovirus, M. pneumonia

Less common: strep pneumonia, staph aureus, H. influenza, Strep agalactiae

32
Q

Community acquired pneumonia in adults

A

Most common: strep pneumonia

Less common: M. pneumonia, H. influenza, Chlamydia pneumonia, respiratory viruses

33
Q

Diagnosis of community & hospital acquired pneumonia

A

Based on the clinical setting of the patient

34
Q

Hospital acquired pneumonia

A

GNR, Strep pneumoniae, Staph aureus, H. influenzae, Legionella spp.

Microbiology 484 (15 decks)

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