Unit 2 Flashcards
history of organizing life
Carlus Linnaeus (sweden) established modern taxonomy (bionomial nomenclature)
taxonomy
naming and classifying organisms
phylogeny
the evolutionary relationships among organisms or their genes
What are the 5 hypotheses portrayed in the phylogenetic tree?
1- morphology 2- paleontology 3- behavior 4- development 5- molecular
morphology
anatomy, external structures, more in common (closer evolutionary relationship)
limitations of morphology
difficult to compare distantly related species and some variation caused by environment
ex- leopard frogs N and Central America
paleontology
includes when are where organisms lived (ancestral vs derived traits)
limitations of paleontology
fossil record- few and fragmented
behavior
active morphology
cultural vs inherited traits (bird and frog calls)
developmental patterns
sea squirts and vertebrae both have notochord
molecular data
DNA often used to construct phylogenetic trees
- mutations accumulate over time
- fewer differences= more closely related
what are photogenically analyses important for?
studying the transmission of viruses
how to (not) read a phylogenetic tree
time is read from root to tip
nodes are speciation events
relatedness = most recent common ancestor
Basic principle of molecular clock
Average rate at which a given gene or protein accumulates changes- used to gauge time of particular split in phylogeny
What does the molecular clock help with?
Dating evolutionary events
-rates constant so they can be used to predict divergence times
What is an example of what a molecular clock can help with?
Timing of when HIV first entered human pop from chimp
What is 16s rRNA? What does it code for?
- Small ribosomal subunit
- useful for comparing bacteria (common among all)
Biological species concept
Species are groups of actually or potentially interbreeding natural populations which are reproductively isolated from other such groups
Limitations of biological species concept
Not asexual/ limited to certain point
Morphological species concept
Appearance
Limitations of morphological species concept
Young, look alike, cryptic species
Group of organisms that can mate with one another and produce fertile offspring
Species
Divergence of biological lineages and the emergence of reproductive isolation
Speciation
Clade
Include all species linked by descent from a common ancestor
Monophyletic
Clade
Polyphyletic
Missing common ancestor
Paraphyletic
Missing some descendants
Taxon
Any species or group that we designate or name
How are new species formed?
Reproductive isolation between species
Reproductive isolation
No longer exchange genes
Reproductive isolation only affects
Sexually reproducing organisms
Allopatric
Populations separated by physical barrier
Examples of allopatric speciation
Continents drift, sea level changes, glaciers, climate changes
(Ozarks vs eastern highlands- fish)
Sympatric speciation
No physical isolation
Pre zygotic isolating mechanisms
Before fertilization occurs
3 examples of prezygotic isolating mechanisms
- mechanical
- behavior
- temporal
Mechanical isolation
Differences in size and shape of reproductive organs makes mating impossible
Behavior isolation
Individuals reject to fail to recognize potential mating partners
Temporal isolation
Mating periods do not overlap
Post zygotic isolating mechanisms
Reduce fitness of hybrid offspring
Examples of post zygotic isolating mechanisms
- low hybrid fitness
- zygotes or adult offspring have lower fitness or hybrids are infertile
Similarities between eukaryotic and bacterial cells
- have cytoplasm
- have plasma membranes
- need to divide to produce more cells
- carry DNA on chromosomes
- copy DNA
Three differences antibiotics exploit
- ribosomes
- prokaryotes have circular DNA
- cell wall
Explain ribosomal difference
- prokaryotes have smaller ribosomes than eukaryotes (70s)
- both have rRNA and proteins =subunits of ribosome
- antibiotics bind to pockets in subunits of ribosomes
Describe difference of prokaryotes shape of DNA
- need DNA gyrase
- –uncoils DNA during replication and prevents “knots”
- –halts DNA replication, kills cell
Describe the difference of the cell wall
Made of peptidoglycan in bacteria
–use to break bacteria into two groups (gram + and -)
How do bacteria form communities?
Aggregate into bio films
How do bacteria reproduce?
Asexually - binary fission
Bacteria 2 locations of DNA
- bacterial chromosome (circular DNA)
- plasmids (DNA connected to chromosome)
Survival strategies of bacteria
- bacteria aggregate into bio films, surrounded by protective slime
- endospores
Endospores
- Produced by bacillus when nutrients are depleted
- resistant to desiccation, heat, and chemicals
- resistant to harsh conditions (anthrax)
Shapes of bacteria
Spirilla (spirals)
Bacilli (rods)
Cocci (sphere)
Size of bacteria
Human hair (100 um)- eukaryotes (60 um) - prokaryotes (1 um) - viruses (0.1 um)
Arrangement of bacterial cells- pairs
Diplococci and diplobacilli
Arrangement of bacterial cells- clusters
Staphylococci
Arrangement of bacterial cells- chains
Streptococci, streptobacilli
Principle of gram stain
Separate bacteria into 2 groups via differential stain
Explain gram stain
Gram +: peptidoglycan = thick and outside (purple)
Gram -: peptidoglycan = think and covered by another layer (pink)
How do we currently define a “species” of bacteria?
- no widely accepted concept
- sequence common 16s rRNA and define cut offs of 95-97%
- operational taxonomic units (otu)
What are the four phyla common the gut?
- actinobacteria
- bacterioidetes
- proteobacteria
- firmicutes
Actinobacteria
- high GC content
- most antibiotics
- ex: streptomyces
Bacterioidetes
-gram negative
-rod: bacilli
-non spore forming
Ex) bacteriodes
Proteobacteria
Holds most sp
Ex) E. coli and pestis (plague)
Firmicutes
Low GC
Endospores
Ex) b anthrecis, clostridium
C diff and fecal transplants that restore microbial diversity
Services bacteria perform for us
Gut microbes affect heart meds
Crime
Brain
Microbes allow energy to be salvaged from otherwise indigestible material
Microbiome
Communities of bacteria that live in and on our bodies
Obligate anaerobes
Oxygen is poisonous to them
Obligate aerobes
Cannot survive without oxygen
Facultative anaerobes
Can shift metabolism between aerobic and anaerobic
Aerotolerant anaerobes
Not damaged by oxygen but do not conduct cellular respiration
Bacteria are (blank)
Natures recyclers
Energy
Photo or Chemo troph
Carbon
Hetero/auto troph
Some plants associate with bacteria to form cooperative nitrogen fixing root nodules. What are these called?
Chemoheterotrophs
Evolutionary reversal
3 generations, reverting back to prior generation (skip generation) and ancestral trait
Evolutionary convergence
Independently evolved traits subjected to similar selection pressures and may become superficially similar (wings)
Homoplasy
Similar traits in distantly related taxa generated by convergent evolution or evolutionary reversals
Tips
Taxa
Nodes
Splits in branches that indicate a division of one lineage into two
synapomorphy
derived traits shared among a group of organisms and viewed as evidence of common ancestry
history of evolutionary relationships among organisms or their genes
phylogeny
root
common ancestor of all the organisms in the tree
any species or group of species that we designate or name
taxon
any taxon that consists of an ancestor and all of its evolutionary descendants
clade
complete evolutionary history of life
tree of life
homologous
any features shared by two or more species that have been inherited from a common ancestor
example of homologous feature
vertebral column in vertebrates
parsimony
simplest explanation and requires fewest homoplasies
characteristics of viruses
not cellular, evolve independently, tiny size, rapid mutation rate, no fossils, diverse
negative sense single stranded RNA viruses
- RNA that is the complement of the mRNA needed for protein translation
- few genes
- RNA dependent RNA polymerase allows them to make complementary mRNA from negative sense RNA genome
- don’t represent a distinct taxonomic group but instead a particular process of cellular escape
examples of negative sense single stranded RNA viruses
measles, mumps, rabies, influenza
positive sense single stranded RNA viruses
- already set for translation (no replication needed)
- most abundant and diverse
examples of positive sense single stranded RNA viruses
diseases in crop plants, polio, hepatitis C, common cold
double stranded RNA virus
- evolved repeatedly from single stranded RNA ancestors
- not closely related
- infect tree of life
example of double stranded RNA virus
infant diarrhea
RNA retroviruses
- genomes composed of SS RNA evolved as escaped cellular components
- regenerate themselves by reverse transcription
- provirus= integrated retroviral DNA
- only infect vertebrates
examples of RNA retroviruses
HIV, cancer
double stranded DNA genome
- polyphyletic
- exchange of modules complicates history
- represent highly reduced parasitic organisms that have lost their cellular structure and ability to survive as free living
examples of double stranded DNA genome
smallpox and herpes
bacteriophages
viruses that attack bacteria
phage therapy
bacteriophage taken from stool and used to treat bacterial infections of the skin and intestines
bacteriophages provide the ability to
evolve and combat antibiotic-resisting bacteria
steps in transition from prokaryotic to eukaryotic cell
1) origin of a flexible cell surface (lost cell wall)
2) cytoskeletons devloped
3) phagocytosis
4) mitochondria and chloroplasts
origin of a flexible cell surface
- can grow larger
- surface area to volume decreases but infolding increases surface area
cytoskeletons developed
- provide cell support
- move materials
- distribute chromosomes
phagocytosis
-loss of cell wall= ability to engulf other organisms in digestive vacuoles
mitochondria and chloroplasts
engulfed bacteria evolved into m and c, endosymbiosis
differences between archaea and bacteria/eukarya
- over half genes unique
- cell membrane (distinctive lipids)
- ether linkages- branched
endosymbiotic theory
- photosynthetic bacteria to chloroplasts
- aerobic bacteria to mitochondria
certain organelles are the descendants of prokaryotes englufed, but not digested by early eukaryotic cells
endosymbiotic theory
primary endosymbiotic theory
engulfment of one cyanobacterium by a larger eukaryotic cell
support of endosymbiotic theory
size and shape, DNA circular, ribosomes (70s)
dinoflagellates
- most photosynthesize
- two flagella
- coral bleaching
- most photosynthesize
- two flagella
- coral bleaching
dinoflagellates
coral bleaching
live endosymbiotically in the cells of corals= reduces corals food when die
apicomplexans
- all parasites
- ex) plasmodium (malaria) and toxoplasma (toxoplasmosis)
- all parasites
- ex) plasmodium (malaria) and toxoplasma (toxoplasmosis)
apicomplexans
ciliates
- most are heterotrophic
- cilia used for locomotion
- ex) paramecium
- contractile vacuole
- hypotonic (more water out than in)
endocytosis
engulf solid food
contractile vacuole
expel water so doesn’t explode
- most are heterotrophic
- ex) paramecium
- contractile vacuole
- hypotonic (more water out than in)
ciliates
diatoms
- unicellular or filaments
- photosynthetic (1/5 of worlds photosynthetic carbon fixation)
- symmetric (bilaterally or radially)
- unicellular or filaments
- photosynthetic (1/5 of worlds photosynthetic carbon fixation)
- symmetric (bilaterally or radially)
diatoms
radiolarians
- radial symmetry
- thin, stiff psuedopods
- elaborate skeletons
- radial symmetry
- thin, stiff psuedopods
- elaborate skeletons
radiolarians
foraminiferans
- shells of calcium carbonate
- accumulate to form limestone
- shells of calcium carbonate
- accumulate to form limestone
foraminiferans
heterolobosean
- ex) Naegleria fowleri (brain eating amoeba)
- amoeboid body form
Euglenids
- have flagella
- some photosynthetic
- have flagella
- some photosynthetic
Euglenids
- ex) Naegleria fowleri (brain eating amoeba)
- amoeboid body form
heterolobosean
kinetoplastids
- parasites
- ex) trypanosoma
- disease: chagas, sleeping sickness
- parasites
- ex) trypanosoma
- disease: chagas, sleeping sickness
kinetoplastids
loboseans
- phagocytosis
- no flagella, cilia
- have lobe shaped pseudopods to move
- phagocytosis
- no flagella, cilia
- have lobe shaped pseudopods to move
loboseans
plasmodial slime molds
- no plasma membrane
- mass of cytoplasm, many nuclei
- streams over substrate- eating
- transforms into fruiting structures
- sporangia hold haploid spores
- no plasma membrane
- mass of cytoplasm, many nuclei
- streams over substrate- eating
- transforms into fruiting structures
- sporangia hold haploid spores
plasmodial slime molds
cellular slime molds
- retain plasma membrane, individuality
- haploid
- retain plasma membrane, individuality
- haploid
cellular slime molds
a multicellular (blank) spore forming organism that gives rise to a multicellular (blank) gamete forming organism
diploid (2n), haploid (n)
the (blank) is the multicellular diploid generation
sporophyte
the (blank) is the multicellular haploid generation
gametophyte
cells in the sporophyte divide (blank) to produce haploid spores
meiotically
spores germinate and divide meitically to produce the (blank)
haploid gametophyte generation
multicellular, diploid, spore producing stage gives rise to (blank)
a multicellular, haploid, gamete producing stage
specialized cells of the diploid spore producing organism (sporophytes) divide (blank) to produce (blank)
meiotically, four haploid spores
gametes produced by the (blank) must fuse to form a new sporophyte generation
(gametophyte generation)
competition
- and -, both harmed
predation
+ and -, one benefits, one harmed
mutualism
+ and +, both benefit