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MT6320 BACTE > U3 LEC: COLONIAL MORPHOLOGY > Flashcards

U3 LEC: COLONIAL MORPHOLOGY Flashcards

1
Q

Importance of Colonial Morphology as a Diagnostic Tool

A
  • provide presumptive diagnosis
  • enchanced quality of patient care through rapid results and cost-effectiveness
  • plays a significant role in quality control
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2
Q

Observed in media immediately surrounding or underneath the colony

A

Hemolysis

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3
Q

Hemolysis

Most important in presumptive identification of?

A

Streptococcus

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4
Q

Hemolysis

Uses what light source?

A

Transillumination (light source behind the plate)

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5
Q

Hemolysis

Two major types

A

a-hemolysis
B-hemolysis

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6
Q

Hemolysis

Partial clearing of blood, green discoloration

A

a-hemolysis

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7
Q

Hemolysis

Examples of a-hemolytic bacteria

A

Streptococcus pneumoniae, viridans strep

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8
Q

Hemolysis

Complete clearing of blood cells

A

B hemolysis

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9
Q

Hemolysis

Examples of B hemolysis

A
  • S. pyogenes
  • S. agalactiae
  • Listeria monocytogenes
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10
Q

Hemolysis

Non hemolytic colonies are referred to as?

A

y hemolytic

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11
Q

Colony size

A
  • Large
  • Medium
  • Small
  • Pinpoint
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12
Q

This helps suggest what you might find on Gram stain

A

Colony Size

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13
Q

Colony Size

Gram-positive is ____ than gram-negative

A

less

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14
Q

Colony Size

Staphylococcus is _____ than Streptococcus

A

greater

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15
Q

Edge of colonies

A
  • Smooth
  • Filamentous
  • Rough
  • Rhizoid
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16
Q

This bacteria has filamentous edge of colonies.

A

Bacillus anthracis

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17
Q

These bacterial colonies have rough edges.

A

Diphtheroid

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18
Q

Form or Margin

This is referred to as a hazy blanket of growth on surface.

A

Swarming

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19
Q

Form or Margin

Examples of bacteria with Swarming colonies

A

Proteus spp.

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20
Q

Description of a colony in which light can pass through

A

Transluscent colony

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21
Q

T/F: There is no such thing as colorless or transparent colony.

A

True

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22
Q

Elevation

A
  • Raised
  • Convex
  • Flat
  • Umbilicate
  • Umbonate
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23
Q

Elevation

Raised flat top

A

Raised

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24
Q

Elevation

Dome shaped

A

Convex

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25
Q

Elevation

Not raised

A

Flat

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26
Q

Elevation

Convex with depressed center (pitting)

A

Umbilicate

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27
Q

Elevation

Examples of Umbilicate

A

S. pneumoniae (if no capsule)

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28
Q

Elevation

Convex with protruding nipple

A

Umbonate

29
Q

Elevation

Examples of Umbonate

A

Diphtheroids

30
Q

Density

A
  • Transparent
  • Transluscent
  • Opaque
31
Q

Semiopaque (bull’s eye colony)

A

Group B strep (mostly Staph and Gr (-) rods)

32
Q

Color

Coagulase negative staph

A

White

33
Q

Color

Enterococcus and most gram-negative rods

A

gray

34
Q

Color

Micrococcus and Neisseria

A

yellow or off-white

35
Q

Color

Diphtheroids

A

buff

36
Q

This is determined by touching a colony with a loop.

A

Consistency

37
Q

Consistency

A
  • Brittle (splinters)
  • Creamy
  • Dry
  • Waxy
  • Sticky
38
Q

Consistency

Entire colony comes off the plate

A

Sticky

39
Q

Consistency

Creamy

A

Staphyloccocus aureus

40
Q

Consistency

Sticky

A

Neisseria

41
Q

Consistency

Brittle

A

Nocardia

42
Q

Consistency

Dry

A

Streptococci

43
Q

Consistency

Dry and waxy

A

Diphtheroid

44
Q

Pigment

Green or green metallic sheen

A

Pseudomonas aeruginosa

45
Q

Pigment

Brick red

A

Serratia marcescens

46
Q

Pigment

Blue

A

Kluyvera

47
Q

Pigment

Purple

A

Chromobacterium violaceum

48
Q

Pigment

Brown-black anaerobe

A

Prevotella melaninogenica

49
Q

Odor

Old sock

A

S. aureus

50
Q

Odor

Fruity or grape-like

A

P. aeruginosa

51
Q

Odor

Putrid

A

Proteus mirabilis

52
Q

Odor

Musty basement

A

Haemophilus spp.

53
Q

Odor

Freshly plowed field

A

Nocardia spp.

54
Q

Organisms in Liquid Media

Streamers

A
  • Vinelike growth
  • Scumlike growth
55
Q

Organisms in Liquid Media

Vinelike growth, puff ball-like

A

Streptococci

56
Q

Organisms in Liquid Media

Scumlike growth

A

Yeast

57
Q

Organisms in Liquid Media

Refers to overall cloudiness of liquid

A

Turbidity

58
Q

Organisms in Liquid Media

Gas bubbles present

A

Enterics

59
Q

Oxidative processes require?

A

oxygen

60
Q

What medium is used in Carbohydrate oxidation and fermentation?

A

Oxidative-fermentative medium

61
Q

This determines the ability of bacteria to produce enzymes

A

Amino acid degradation

62
Q

What medium is used for amino acid degradation?

A

Lysine Iron Agar (LIA)

63
Q

Enzymes in amino acid degradation can:

A
  • Deaminate
  • Dihydrolyze
  • Decarboxylate
64
Q

Level 1: Critical/invasive

A
  • Amniotic fluid
  • blood
  • brain
  • CSF
  • heart valve
  • pericardial fluid
65
Q

Level 2: Unpreserved

A
  • Bloody fluids
  • Bone
  • wound drainage
  • feces
  • sputum
  • tissue
66
Q

Level 3: Quantitation Required

A
  • Catheter tip
  • Urine
  • Tissue for quantification
67
Q

Level 4: Preserved

A
  • Feces in preservative
  • Urine in preservative
  • Swabs in holding medium
68
Q

Traditional Biochemical Testing Panel

A
  • Citrate
  • TSI
  • LIO
  • MIO/SIM
  • Urease
  • Glucose
  • Lactose
  • Maltose

MT6320 BACTE (13 decks)

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