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MT6320 BACTE > U3 LAB: MICROSCOPIC MORPHOLOGY > Flashcards

U3 LAB: MICROSCOPIC MORPHOLOGY Flashcards

1
Q

Uses one stain

A

Simple stain

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2
Q

Provides morphology and arrangement

A

Simple stain

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3
Q

Example of simple stain

A

Cocci in chains

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4
Q

Commonly used in Bacteriology

A

Differential stain

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5
Q

Provides Gram reaction, morphology, and arrangement

A

Differential stain

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6
Q

Primary stain

A

Crystal violet

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7
Q

Mordant

A

Gram’s iodine

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8
Q

Use of mordant

A

enhances primary stain to organism

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9
Q

Decolorizer

A

acetone alcohol

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10
Q

Use of decolorizer

A

removes primary stain

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11
Q

Secondary stain

A

Safranin

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12
Q

No mordant

A

secondary stain will be observed (pink)
- erroneous result
- false negative result

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13
Q

No alcohol

A

purple
- false positive result

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14
Q

Preparation of smear from clinical specimens

A

Direct smear

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15
Q

Cells observed in Direct Smear

A
  • Bacterial cells
  • RBCs, WBCs
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16
Q

Preparation of smear from culture

A

Indirect smear

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17
Q

Comma shaped bacilli

A

Vibrio

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18
Q

Not usually encountered, gram stain is not commonly done

A

Spiral

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19
Q

Gram (+) cocci in tetrads

A

Micrococcus tetragena

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20
Q

Gram (+) cocci in pairs (lancet shaped)

A

Streptococcus pneumoniae

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21
Q

Gram (-) cocci in pairs (may be found intracellularly inside PMN, coffee bean shaped)

A
  • Neisseria sp. (N. meningitidis, N. gonorrhoeae)
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22
Q

Gram (+) cocci in sarcinae (cubical packet)

A

Sarcina lutea

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23
Q

Gram (+) cocci in chains

A

Streptococcus

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24
Q

Gram (+) cocci in clusters

A

Staphylococcus

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25
Q

Gram (+) bacilli in chains (large bacilli with square cut ends)

A

Bacillus

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26
Q

Bamboo fishing pole appearance

A

Bacillus

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27
Q

Clear structures in Bacilli

A

Endospores

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28
Q

Gram (-) bacilli in pairs

A

Pseudomonas or non-fermenters

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29
Q

Refers to elongated cocci

A

Coccobacilli

30
Q

Gram (-) coccobacilli in pairs, singles

A

Enterics

31
Q

Gram (+) bacilli that are large, arranged singly or inidividually with spores

A
  • Clostridium tetani
  • Clostridium botulinum
32
Q

Spore of C. tetani is located?

A

terminally

33
Q

Box car appearance

A

Clostridium perfringens

34
Q

Gram (-) coccobacilli straight and rod shaped

A

Haemophilus ducreyi

35
Q
  • smallest pathogenic bacilli
  • school of fish / railroad track pattern
A

Haemophilus ducreyi

36
Q

Gram (-) bacilli with rounded ends

A

Bacteroides fragilis

37
Q

Gram (+) large irregular bacilli

A

Corynebacterium

38
Q

“club shaped” , Chinese Character, X or Y, palisade, snapping arrangement

A

Corynebacterium

39
Q

Gram (+) branching bacilli with bifurcated or spatulated ends

A

Bifidobacterium

40
Q

Gram (+) slender, branching, filamentous bacilli

A

Nocardia

41
Q

T/F: Nocardia is also partially acid fast.

A

True

42
Q

Gram (+) filamentous bacilli with a V-shaped pattern

A

Erysipelothrix rhusiopathiae

43
Q

Erysipelothrix rhusiopathiae is the causative agent of?

A

Red disease

44
Q

Gram (-) bacilli in long looped filamentous forms

A

Streptobacillus moniliformis

45
Q

Streptobacillus moniliformis is also known as?

A

necklace bacillus

46
Q

Gram (-) fusiform bacilli

A

Fusobacterium nucleatum

47
Q

Gram (-) comma/curved shaped bacilli in singles

A

Vibrio

48
Q

Gram (-) seagull wing shaped, S shaped bacilli

A

Campylobacter

49
Q

Gram (-) curved bacilli with two or more curves

A

Spirillum

50
Q

On gram stain, AFB is?

A

weakly gram positive

51
Q

Acid Fast Bacilli Slender

Serpentine cord like pattern

A

Mycobacterium tuberculosis

52
Q

AFB

Primary stain

A

Carbol fuchsin

53
Q

AFB

Mordant

A

Heat / Tergitol

54
Q

AFB

Decolorizer

A

Acid alcohol

55
Q

AFB

Secondary stain

A

Methylene blue / Malachite green

56
Q

Acid Fast Bacilli Slender

Palisades / globi packets / cigar bundle, arranged singly

A

Mycobacterium leprae

57
Q

M. leprae is a causative agent of?

A

Leprosy

58
Q

What microscope is used for Spirochetes?

A

Darkfield microscopy

59
Q

What stains are used for Spirochetes?

A
  • silver stains
  • fluorescent stains
  • tissue stains (Giemsa)
60
Q

Tightly twisted, with one end or both bent to a hook

A

Leptospira interrogans

61
Q

Leptospira interrogans resemble a?

A

Question mark

62
Q

Motility of T. pallidum

A

Corkscrew motility

63
Q

Flexibly twisted

A

Borrelia

64
Q

This is the only spirochete that can be stained with Giemsa

A

Borrelia

65
Q

Yeast cells are Gram ___

A

positive

66
Q

Square shaped bacteria

A

Haloarcula spp.

67
Q

Starshaped bacteria

A

Stella spp.

68
Q

DSSM stands for?

A

Direct Sputum Smear Microscopy

69
Q

What should be read before reporting sputum smear negative for ABF?

A

300 OIF

70
Q

Structures seen in sputum smear

A
  • WBC (pus cells)
  • Squamous epithelial cells
  • Mucus threads
71
Q

Spiral shaped structures that can be seen in asthmatics

A

Curschmann’s Spirals

MT6320 BACTE (13 decks)

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