U1 LAB: LAB SAFETY OVERVIEW Flashcards
Potential hazards in the work setting
- Electric shock
- Toxic gas
- Flammable liquids
- Radioactive materials
- Corrosive substances
- Mechanical trauma
- Infectious agents
Laboratory safety requires what of all hazards?
effective control
Working area must be cleaned with?
1:10 dilution of 5% sodium hypochlorite
Spillage must be cleaned with?
1:5 dilution of 5% sodium hypochlorite
Alternative to bleach
Diluted Lysol solution
Plastic bag for infectious and contaminated materials
Yellow plastic bag
Plastic bag for noninfectious dry mats
Black plastic bag
Plastic bag for noninfectious wet mats
Green plastic bag
Container for contaminated sharps
Red puncture proof
What should be worn if splashes are anticipated?
Face shield or laboratory goggles
T/F: PPE must be worn inside the bacte lab only and must be removed when leaving the premises.
True
Route of Infection
Aerosols from centrifugation of unstoppered tubes, heating cultures too rapidly, removing stoppers from tubes, leakage from container
Airborne
Route of Infection
Result of failure to wash hands, eating, drinking, smoking, cosmetics, or mouth pipetting
Ingestion
Route of Infection
Result from needlesticks, broken glass, animal bites, small scratches
Direct inoculation
Route of Infection
Directly enter through mucous membranes
Mucous membrane contact
Route of Infection
Ticks, fleas, mosquitoes
Arthropod vectors
Safety Education
- Orientation and continuing education
- Safety manual
Universal Precautions
CDC (1987) set of preventive measures to reduce HIV, HBV, and other bloodborne pathogens, and is applied to all human blood and fluids that contain visible blood
Standard Precautions
All blood, tissue, body fluid, secretions and excretions (Except sweat) are considered potentially infectious
These are devices that isolate or remove a hazard
Engineering Controls
Engineering Controls in the Lab
- Sharps disposal containers
- Self-sheathing needles
- Sharps with engineered sharps injury protections
- Needless systems
- Chemical fume hoods
- Splash guards
- Barriers
- Biologic safety cabinets
- Monitoring devices
- Safety interlocks
This is a device that encloses a workspace in such a way as to protect workers from aerosol exposure to infectious disease agents.
Biosafety Cabinet