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MT6320 BACTE > U1 LAB: LAB SAFETY OVERVIEW > Flashcards

U1 LAB: LAB SAFETY OVERVIEW Flashcards

1
Q

Potential hazards in the work setting

A
  • Electric shock
  • Toxic gas
  • Flammable liquids
  • Radioactive materials
  • Corrosive substances
  • Mechanical trauma
  • Infectious agents
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2
Q

Laboratory safety requires what of all hazards?

A

effective control

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3
Q

Working area must be cleaned with?

A

1:10 dilution of 5% sodium hypochlorite

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4
Q

Spillage must be cleaned with?

A

1:5 dilution of 5% sodium hypochlorite

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5
Q

Alternative to bleach

A

Diluted Lysol solution

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6
Q

Plastic bag for infectious and contaminated materials

A

Yellow plastic bag

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7
Q

Plastic bag for noninfectious dry mats

A

Black plastic bag

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8
Q

Plastic bag for noninfectious wet mats

A

Green plastic bag

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9
Q

Container for contaminated sharps

A

Red puncture proof

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10
Q

What should be worn if splashes are anticipated?

A

Face shield or laboratory goggles

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11
Q

T/F: PPE must be worn inside the bacte lab only and must be removed when leaving the premises.

A

True

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12
Q

Route of Infection

Aerosols from centrifugation of unstoppered tubes, heating cultures too rapidly, removing stoppers from tubes, leakage from container

A

Airborne

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13
Q

Route of Infection

Result of failure to wash hands, eating, drinking, smoking, cosmetics, or mouth pipetting

A

Ingestion

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14
Q

Route of Infection

Result from needlesticks, broken glass, animal bites, small scratches

A

Direct inoculation

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15
Q

Route of Infection

Directly enter through mucous membranes

A

Mucous membrane contact

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16
Q

Route of Infection

Ticks, fleas, mosquitoes

A

Arthropod vectors

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17
Q

Safety Education

A
  • Orientation and continuing education
  • Safety manual
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18
Q

Universal Precautions

A

CDC (1987) set of preventive measures to reduce HIV, HBV, and other bloodborne pathogens, and is applied to all human blood and fluids that contain visible blood

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19
Q

Standard Precautions

A

All blood, tissue, body fluid, secretions and excretions (Except sweat) are considered potentially infectious

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20
Q

These are devices that isolate or remove a hazard

A

Engineering Controls

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21
Q

Engineering Controls in the Lab

A
  • Sharps disposal containers
  • Self-sheathing needles
  • Sharps with engineered sharps injury protections
  • Needless systems
  • Chemical fume hoods
  • Splash guards
  • Barriers
  • Biologic safety cabinets
  • Monitoring devices
  • Safety interlocks
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22
Q

This is a device that encloses a workspace in such a way as to protect workers from aerosol exposure to infectious disease agents.

A

Biosafety Cabinet

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23
Q

This is a form of containment, and sterilizes by heat, UV light, or HEPA filter.

A

Biosafety Cabinet

24
Q

HEPA stands for?

A

High Efficiency Particulate Air

25
Q

Biosafety Cabinet

Only the air to be exhausted is sterilized

A

Class I

26
Q

T/F: BSC Class I protects sample from contamination.

A

False

27
Q

Biosafety Cabinet

Sterilizes air that flows over infectious material, and air to be exhausted

A

Class II

28
Q

Biosafety Cabinet

Self-contained, 70% of air is recirculated in work area

A

Class II, Type A

29
Q

Biosafety Cabinet

Discharged out of the building

A

Class II, Type B

30
Q

Biosafety Cabinet

Operate either Type A when in recirculating mode or Type B when exhausting

A

Class II, Type C

31
Q

Biosafety Cabinet

Completely enclosed, ventilated, with negative pressure through HEPA filters

A

Class III

32
Q

What is the minimum requirement BSC?

A

Type II, Class A

33
Q

T/F: PPE is the least preferred to contain hazard.

A

True

34
Q

Biosafety Level

No known pathogenic potential for immunocompetent individuals (healthy, no infections)

A

Level 1

35
Q

Biosafety Level 1 examples

A

Bacillus subtilis

36
Q

Biosafety Level

Most common microorganisms associated with laboratory-acquired infections

A

Level 2

37
Q

Biosafety Level 2 examples (Direct contact)

A

HBV, HIV, Staphylococcus, Salmonella, Shigella

38
Q

Biosafety Level

Mycobacterium tuberculosis, Brucella, Coccidoides immitis, Rickettsia, Arbovirus (Inhalation)

A

Level 3

39
Q

T/F: In BSL 3, air movement must be carefully controlled.

A

True

40
Q

Biosafety Level

Exotic viruses such as filovirus and arenavirus

A

Level 4

41
Q

Process that kills all forms of microbial life, including bacterial endospores

A

Sterilization

42
Q

Most resistant stage

A

Endospore

43
Q

Process that destroys pathogenic organisms, but not all

A

Disinfection

44
Q

Removal of pathogenic microorganisms

A

Decontamination

45
Q

Factors influencing the degree of killing

A
  • Types of organisms
  • Number of organisms
  • Concentration/compatibility of disinfectant
  • Presence of organic material
  • Nature of surface to be disinfected
  • Contact time
  • Temperature
  • pH
    -Biofilm
46
Q

Appropriate sterilization technique for biohazardous wastes

A

Autoclave

47
Q

T/F: All microbiological wastes can go straight to disposal after putting in 2 leak-proof bags.

A

False

48
Q

Instruments, appliances must be checked for hazards at least once every?

A

12 mos

49
Q

MSDS Section I

A

Manufactuere details, date prepared

50
Q

MSDS Section II

A

Hazardous ingredient’s information, PEL

51
Q

MSDS Section III

A

Physical / Chemical Characteristics

52
Q

MSDS Section IV

A

Fire and explosion hazard data

53
Q

MSDS Section V

A

Reactivity data

54
Q

MSDS Section VI

A

Health hazard data

55
Q

MSDS Section VII

A

Precautions

56
Q

MSDS Section VIII

A

Control Measures

MT6320 BACTE (13 decks)

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