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MT6320 BACTE > U3 LEC: BACTERIAL CULTIVATION > Flashcards

U3 LEC: BACTERIAL CULTIVATION Flashcards

1
Q

Microscopic examination of specimens provide preliminary information about a bacterial infection.

A

Bacterial Cultivation

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2
Q

Part of Koch’s postulate

A

What is seen in sample microscopy should be observed in sample culture

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3
Q

This provides definitive ID of a particular agent

A

Cultivation and isolation

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4
Q

Flow of Traditional ID

A
  1. Sample collection
    2, Microscopy (stained/unstained)
  2. Culture
  3. Microscopy of Culture
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5
Q

Flow of Alternative Bacterial ID

A
  1. Sample collection
  2. Culture
  3. Molecular testing
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6
Q

Normal flora of the throat

A

Moraxella catarhalis

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7
Q

One of the most sophisticated biochemical testing, just highly presumptive (not definitive and confirmatory)

A

VITEK

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8
Q

VITEK is best for?

A

Drug Susceptibility and Antimicrobial Susceptibility Testing

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9
Q

Purposes of bacterial cultivation

A
  • grow and isolate all bacteria
  • determine bacteria that are most likely causing infection
  • obtain sufficient growth of clinically relevant bacteria
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10
Q

In vitro

A

artificial

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11
Q

In vivo

A

animals or human

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12
Q

In situ

A

environment

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13
Q

Key Terms

To introduce

A

Inoculation

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14
Q

Key Terms

Media that has the organism

A

Inoculum

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15
Q

Other Key Terms

A
  • Incubation
  • Cultures
  • Colonies
  • Isolation
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16
Q

Criteria for Growth Media

A
  • sufficient moisture/water
  • properly adjusted pH
  • suitable level of oxygen
  • sterile
  • incubated at proper temp
  • free from inhibitory substances
  • must contain essential nutrients in proper concentration
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17
Q

Classification of Growth Media: According to Physical state

A
  • Liquid
  • Semi-solid
  • Solid
  • Biphasic media
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18
Q

This media can be observed in the blood culture bottle.

A

Biphasic Media

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19
Q

This is the first solidifying agent used.

A

Gelatin

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20
Q

At this percent, it can be used to detect presence of gelatinase.

A

12%

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21
Q

Red algae

A

Rhodophyta

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22
Q

Characteristics of Solid Media

A
  • not degradable
  • liquid at 55-60C
  • solid at 50C or lower
  • melts at high temperature (95-100C; Hysteresis)
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23
Q

According to dispensing/formation

A
  • Plated medium
  • Tubed
  • Bottled
  • Biphasic
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24
Q

Proper form of dispensed media

A

smooth, equal, molten liquid, no bubble formation, not too thin or thick

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25
Q

Kirby-Bauer Method

A

Mueller-Hilton Agar: neutral pH, 0.5 McFarland Standard, 4mm thick

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26
Q

This media can be used 5 days from preparation, prone to drying and contamination.

A

Plated Medium

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27
Q

T/F: If there is still liquid in plated media, you can still use it.

A

True

pwede pa basta moist environment

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28
Q

Screw Cap tubes lasts up to?

A

2 weeks to 1 month

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29
Q

Tubes with cotton lasts up to?

A

2 weeks

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30
Q

Matter of Dispensing

Plated

A
  1. Weigh
  2. Dissolve
  3. Autoclave
  4. Dispense
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31
Q

Matter of Dispensing

Tubed

A
  1. Weigh
  2. Dissolve
  3. Dispense
  4. Autoclave
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32
Q

Manner of Formation

Plated Medium

A
  • Clock Method
  • Radial
  • Simple
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33
Q

Manner of Formation

Butt

A

Stab

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34
Q

Manner of Formation

Slant

A

Streak

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35
Q

T/F: Spreading is equal to seeding, but not equal to streaking.

A

True

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36
Q

Manner of Formation

Few growth in primary streak

A

very little growth

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37
Q

Manner of Formation

Growth only in primary streak

A

Little growth

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38
Q

Manner of Formation

Growth in primary and secondary streak

A

Moderate growth

39
Q

Manner of Formation

Growth in primary, secondary, tertiary streak

A

Heavy growth

40
Q

Manner of Formation

1 to 10 colonies only

A

Rare growth

41
Q

According to Composition

A
  • Chemically Defined / Synthetic
  • Complex / Non-chemically defined
  • Tissue Culture
42
Q

According to Composition

Has defined compositions and concentrations

A

Chemically defined / Synthetic

43
Q

According to Composition

Cannot be defined

A

Complex / Non-chemically defined

44
Q

According to Composition

Examples of Complex / Non-chemically defined

A

Blood agar, Chocolate agar

45
Q

According to Composition

This refers to organized tissues to look like organs, used for cancer treatment and bacterial culture

46
Q

According to function / use

A
  • Basic / Basal / General Purpose
  • Enriched Culture Media
47
Q

According to function / use

Supportive media, for non-fastidious organisms

A

Basic/Basal/General Purpose

48
Q

According to function / use

Fortified with nutrients, can grow some fastidious organisms

A

Enriched Culture Media

49
Q

According to function

A
  • Selective medium
  • Differential medium
50
Q

According to function

Refers to inhibitors

A

Selective Medium

51
Q

According to function

Refers to inhibitors and indicators

A

Differential

52
Q

According to function

This can differentiate one colony from the other

A

Indicators

53
Q

Black colonies, hydrogen sulfide indicator

A

Salmonella agar

54
Q

No hydrogen sulfide indicator

A

Shigella agar

55
Q

Inhibitors for Gr (+) bacteria

A
  • Dyes (gentian violet, crystal violet)
  • Bile Salt (Sodium Desoxycholate)
56
Q

Has crystal violet, selective for Gr (-) bacteria, differentiate for lactose and non-lactose fermenters

A

MacConkey Agar

57
Q

Selective media for Staphylococcus sp.

A

Mannitol Salt Agar

58
Q

According to function

This causes growth of enteric pathogens but will inhibit growth of Gr (+) bacteria

A

Bile Salt (Sodium Desoxycholate)

59
Q

Inhibitors for Gr (-) bacteria

A
  • Sodium azide
  • Potassium tellurite
60
Q

According to function

This is the best preservative for urine samples

A

Sodium azide

61
Q

Selective Culture Media

For TB

A

Lowenstain Jensen Medium

62
Q

Selective Culture Media

Lowenstain Jensen Medium is enriched in?

63
Q

Selective Culture Media

Inhibitor in Lowenstain Jensen Medium

A

Malachite green

64
Q

Selective Culture Media

For Gram Positive (+)

A

Phenylethyl alcohol

65
Q

Selective Culture Media

For Neisseria gonorrhoeae

A
  • Thayer Martin
  • Modified Thayer Martin
  • Martin Lewis
  • New York City Agar
66
Q

Selective Culture Media

Inhibitor for Thayer Martin

A
  • Vancomycin (Gr +)
  • Colistin (Gr -)
  • Nystatin (fungi)
67
Q

Selective Culture Media

Inhibitor for Modified Thayer Martin

A

addition of Trimethoprim lactate

68
Q

Selective Culture Media

Inhibitor for Martin Lewis

A
  • Vancomycin
  • Colistin
  • Trimethoprim lactate
  • Anisomicin (fungi)
69
Q

Selective Culture Media

Inhibitor for New York City Agar

A
  • Amphotericin B (fungi)
70
Q

Mannitol Salt Agar

Inhibitor

71
Q

Mannitol Salt Agar

pH indicator

A

Phenol Red

72
Q

Mannitol Salt Agar

Normal color, and color change

A

Normal: pink
Change: yellow

73
Q

Thiosulfate Citrate Bile Salts Sucrose Agar

Inhibitor

A
  • Thiosulfate
  • Bile salts
  • high pH
74
Q

Thiosulfate Citrate Bile Salts Sucrose Agar

pH indicator

A

Bromthymol blue

75
Q

Thiosulfate Citrate Bile Salts Sucrose Agar

Color of colonies

A

yellow and green

76
Q

According to use

A
  • Enrichment medium
  • Transport medium
  • Characteristic / biochemical characteristic medium
  • Motility medium
  • Antibiotic susceptibility
  • Anaerobic / Reducing culture medium
  • Pharmaceutical agar
  • Media for capnophiles
77
Q

According to use

Increases number of pathogens

A

Enrichment medium

78
Q

According to use

Maintain viability

A

Transport Medium

79
Q

According to use

Under enrichment medium

A
  • Alkaline peptone water
  • Tetrathionate
  • Hafnia or gram negative broth
80
Q

According to use

Under transport medium

A
  • Amies, Cary-blair (Stool)
  • Transgrow
81
Q

According to use

Under biochemical

A
  • Triple Sugar Iron Agar (Gram - bacteria; ability to ferment glucose, lactose or sucrose to produce H2S and gas)
82
Q

According to use

under Motility medium

A

Sulfide Indole Motility (SIM)

83
Q

According to use

under Antibiotic susceptibility

A
  • Mueller Hinton Agar
  • Middle Brook Agar (need high protein)
84
Q

According to use

under Anaerobic

A

Bacteroides anaerobic medium (Additives that reduce O2)

85
Q

According to use

under Pharmaceutical

A

research, no nutrients, pure agar

  • Malunggay leaves
  • Malco agar (coconut water)
86
Q

Manifestations of Growth

Solid Media

A

Appearance of colonies

87
Q

Manifestations of Growth

Liquid media

A
  • Turbidity
  • Presence of Pellicle
  • Clot or coagulation
  • Bubbles
88
Q

Location in broth media

A
  • Surface
  • Column
  • Bottom
89
Q

When should you observe colony morphology?

A

18 to 24 hours postculture

90
Q

This may affect size and characteristics of colonies

A

Age of culture

91
Q

This determines the growth and colony morphology.

A

Plating media

92
Q

Other term for plating media

A

Plate reading

93
Q

T/F: Fastidious organisms require a longer time of incubation.

MT6320 BACTE (13 decks)

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