U3 LAB: COLONIAL MORPHOLOGY

Question 1

Streaking is for?

Answer 1

isolation of organism

Question 2

Importance of ID

Answer 2

determine antibiotic for treatment

Question 3

Urine sample

Answer 3

Quantitative isolation

Question 4

Streaking pattern is considered as?

Answer 4

semi-quantitative determination

Question 5

Score

1+

Answer 5

1: Less than 10

Question 6

Score

2+

Answer 6

1,2: Less than 10

Question 7

Score

3+

Answer 7

1,2: Greater than 10
3: Less than 10

Question 8

Score

4+

Answer 8

1,2,3: Greater than 10
4: Greater than 5

Question 9

What instrument will be used for urine culture?

Answer 9

Calibrated loop

Question 10

Diameter size of calibrated loop for Urine Culture

Answer 10

• 0.001mL
• 0.01mL

Question 11

Hemolysis is seen in?

Answer 11

Blood Agar Plate

Question 12

Partial hemolysis, has a greenish zone

Answer 12

Alpha Hemolysis

Question 13

Complete hemolysis, has a clear zone

Answer 13

Beta hemolysis

Question 14

No hemolysis

Answer 14

Gamma hemolysis

Question 15

Bacteria with Alpha Prime Hemolysis

Answer 15

Streptococci

Question 16

Bacteria with Double Zone of Hemolysis

Answer 16

Clostridium perfringens

Question 17

Size

pinhead

Answer 17

Staphylococci

Question 18

Size

pinpoint

Answer 18

Streptococci

Question 19

Size

Large, Medium

Answer 19

gram negative bacilli

Question 20

Large, rough, hemolytic colonies

Answer 20

Bacillus cereus

Question 21

Swarming colonies

Answer 21

Proteus spp.

Question 22

T/F: Proteus is a flagellated bacteria.

Answer 22

True

Question 23

Colonies with rough edges, dry appearance, and umbonate center

Answer 23

Diphteroid

Question 24

Molar tooth colonies

Answer 24

Actinomyces israelii

Question 25

Dry, Wrinkled breadcrumb like

Answer 25

Nocardia sp.

Question 26

Hammered copper

Answer 26

Yersinia pestis

Question 27

Yersinia pestis is also known as?

Answer 27

Plague bacilli

Question 28

Capsulated

Answer 28

Klebsiella sp.

Question 29

Green, pearlescent appearance

Answer 29

Pseudomonas aerigunosa

Question 30

Pigment

Yellow green color

Answer 30

Pyoverdin

Question 31

Pigment

Blue

Answer 31

Pyocyanin

Question 32

Pigment

Red

Answer 32

Pyorubin

Question 33

Pigment

Brown/Black

Answer 33

Pyomelanin

Question 34

Cauliflower like

Answer 34

Myobacterium tuberculosis

Question 35

Fried egg appearance

Answer 35

Mycoplasma and Ureaplasma

Question 36

Pedicle/Foot like

Answer 36

Candida albicans (not a bacteria)

Question 37

Yellow colony

Answer 37

Staphylococcus aureus

Question 38

Brick-red pigment

Answer 38

Serratia marcescens

Question 39

Pigment of Serratia marcescens

Answer 39

Prodigiosin

Question 40

Blue-green pigment

Answer 40

Pseudomonas aerigunosa

Question 41

Purple colony

Answer 41

Chromobacterium violaceum

Question 42

String test is used for?

Answer 42

mucoid colonies (Capsulated bacteria)

Question 43

Medusa head colonies

Answer 43

Bacillus anthracis

Question 44

Growth in liquid media

All throughout

Answer 44

Facultative aerobe

Question 45

Growth in liquid media

On top of media

Answer 45

Obligate aerobe

Question 46

Growth in liquid media

Bottom of media

Answer 46

Facultative anaerobe

Question 47

Growth in liquid media

Middle

Answer 47

Aerotolerant

Question 48

Vine or Streamer / Puffed balls

Answer 48

Streptococci

Question 49

Produces scum at sides of thioglycollate

Answer 49

Pseudomonas aerigunosa

Question 50

Stalactite

Answer 50

Yersinia pestis

Question 51

Burnt chocolate / chocolate cake

Answer 51

Proteus

Question 52

Corn taco / fruity / corn tortilla

Answer 52

Pseudomonas aerigunosa

Question 53

Old sock

Answer 53

Staphylococcus aureus

Question 54

Musty

Answer 54

Pasteurella multocida

Question 55

Bleach

Answer 55

Eikenella corrodens

Question 56

Almond like

Answer 56

Chromobacterium violaceum

Question 57

Barn yard

Answer 57

Clostridium difficile

Question 58

Popcorn like

Answer 58

Neisseria animaloris

Question 59

Freshly plowed field

Answer 59

Nocardia

Question 60

Musty / musty basement

Answer 60

Haemophilus

Question 61

Freshly cut apple / fruity apple / strawberry

Answer 61

Alcaligenes faecalis

Question 62

Smooth, filamentous, rough/rhizoid

Answer 62

Diphteroid colonies

Question 63

Medusa Head colonies

Answer 63

Bacillus anthracis

Question 64

Fuzzy edged

Answer 64

Eikenella corrodens

Question 65

Wagon-wheel appearance

Answer 65

Moraxella catarrhalis

Question 66

White

Answer 66

Coagulase negative (-) Staphylococci

Question 67

Gray

Answer 67

• Gram (-) rods
• Enterococcus spp.

Question 68

Yellow or Off-white

Answer 68

• Staphylococcus aureus
• Micrococcus spp.
• Non-pathogenic Neisseria

Question 69

Buff

Answer 69

Diphteroids

Question 70

Brittle (forms splinters)

Answer 70

Nocardia spp.

Question 71

Creamy (butyrous)

Answer 71

Staphylococcus aureus

Question 72

Dry and Waxy

Answer 72

Diphteroids

Question 73

Sticky

Answer 73

Neisseria spp.

Question 74

Beaten egg white

Answer 74

Bacillus anthracis

Question 75

• Convex
• Entire edge
• 2-3mm
• Creamy, yellowish
• Beta hemolytic

Answer 75

Staphylococcus

Question 76

• Convex or pulvinate
• Transluscent
• Pinpoint
• Butyrous
• Wide zone of Beta hemolysis

Answer 76

Streptococcus

Question 77

• Umbilicate or flat
• Transluscent
• Butyrous or mucoid
• Broad zone of alpha hemolysis

Answer 77

Pneumococcus

Question 78

• Pulvinate
• semiopaque
• gray
• moist to dry
• beta hemolysis may or may not be present

Answer 78

Escherichia coli and other Enterics

Question 79

• Flat
• gray
• spreading as a thin film on agar
• burnt chocolate

Answer 79

Proteus

Question 80

• Flat
• gray to greenish
• margins erose or spreading
• green-blue pigment
• grapelike odor

Answer 80

Pseudomonas