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MT6320 BACTE > U3 LAB: COLONIAL MORPHOLOGY > Flashcards

U3 LAB: COLONIAL MORPHOLOGY Flashcards

1
Q

Streaking is for?

A

isolation of organism

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2
Q

Importance of ID

A

determine antibiotic for treatment

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3
Q

Urine sample

A

Quantitative isolation

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4
Q

Streaking pattern is considered as?

A

semi-quantitative determination

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5
Q

Score

1+

A

1: Less than 10

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6
Q

Score

2+

A

1,2: Less than 10

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7
Q

Score

3+

A

1,2: Greater than 10
3: Less than 10

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8
Q

Score

4+

A

1,2,3: Greater than 10
4: Greater than 5

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9
Q

What instrument will be used for urine culture?

A

Calibrated loop

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10
Q

Diameter size of calibrated loop for Urine Culture

A
  • 0.001mL
  • 0.01mL
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11
Q

Hemolysis is seen in?

A

Blood Agar Plate

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12
Q

Partial hemolysis, has a greenish zone

A

Alpha Hemolysis

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13
Q

Complete hemolysis, has a clear zone

A

Beta hemolysis

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14
Q

No hemolysis

A

Gamma hemolysis

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15
Q

Bacteria with Alpha Prime Hemolysis

A

Streptococci

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16
Q

Bacteria with Double Zone of Hemolysis

A

Clostridium perfringens

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17
Q

Size

pinhead

A

Staphylococci

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18
Q

Size

pinpoint

A

Streptococci

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19
Q

Size

Large, Medium

A

gram negative bacilli

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20
Q

Large, rough, hemolytic colonies

A

Bacillus cereus

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21
Q

Swarming colonies

A

Proteus spp.

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22
Q

T/F: Proteus is a flagellated bacteria.

A

True

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23
Q

Colonies with rough edges, dry appearance, and umbonate center

A

Diphteroid

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24
Q

Molar tooth colonies

A

Actinomyces israelii

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25
Q

Dry, Wrinkled breadcrumb like

A

Nocardia sp.

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26
Q

Hammered copper

A

Yersinia pestis

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27
Q

Yersinia pestis is also known as?

A

Plague bacilli

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28
Q

Capsulated

A

Klebsiella sp.

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29
Q

Green, pearlescent appearance

A

Pseudomonas aerigunosa

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30
Q

Pigment

Yellow green color

A

Pyoverdin

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31
Q

Pigment

Blue

A

Pyocyanin

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32
Q

Pigment

Red

A

Pyorubin

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33
Q

Pigment

Brown/Black

A

Pyomelanin

34
Q

Cauliflower like

A

Myobacterium tuberculosis

35
Q

Fried egg appearance

A

Mycoplasma and Ureaplasma

36
Q

Pedicle/Foot like

A

Candida albicans (not a bacteria)

37
Q

Yellow colony

A

Staphylococcus aureus

38
Q

Brick-red pigment

A

Serratia marcescens

39
Q

Pigment of Serratia marcescens

A

Prodigiosin

40
Q

Blue-green pigment

A

Pseudomonas aerigunosa

41
Q

Purple colony

A

Chromobacterium violaceum

42
Q

String test is used for?

A

mucoid colonies (Capsulated bacteria)

43
Q

Medusa head colonies

A

Bacillus anthracis

44
Q

Growth in liquid media

All throughout

A

Facultative aerobe

45
Q

Growth in liquid media

On top of media

A

Obligate aerobe

46
Q

Growth in liquid media

Bottom of media

A

Facultative anaerobe

47
Q

Growth in liquid media

Middle

A

Aerotolerant

48
Q

Vine or Streamer / Puffed balls

A

Streptococci

49
Q

Produces scum at sides of thioglycollate

A

Pseudomonas aerigunosa

50
Q

Stalactite

A

Yersinia pestis

51
Q

Burnt chocolate / chocolate cake

A

Proteus

52
Q

Corn taco / fruity / corn tortilla

A

Pseudomonas aerigunosa

53
Q

Old sock

A

Staphylococcus aureus

54
Q

Musty

A

Pasteurella multocida

55
Q

Bleach

A

Eikenella corrodens

56
Q

Almond like

A

Chromobacterium violaceum

57
Q

Barn yard

A

Clostridium difficile

58
Q

Popcorn like

A

Neisseria animaloris

59
Q

Freshly plowed field

A

Nocardia

60
Q

Musty / musty basement

A

Haemophilus

61
Q

Freshly cut apple / fruity apple / strawberry

A

Alcaligenes faecalis

62
Q

Smooth, filamentous, rough/rhizoid

A

Diphteroid colonies

63
Q

Medusa Head colonies

A

Bacillus anthracis

64
Q

Fuzzy edged

A

Eikenella corrodens

65
Q

Wagon-wheel appearance

A

Moraxella catarrhalis

66
Q

White

A

Coagulase negative (-) Staphylococci

67
Q

Gray

A
  • Gram (-) rods
  • Enterococcus spp.
68
Q

Yellow or Off-white

A
  • Staphylococcus aureus
  • Micrococcus spp.
  • Non-pathogenic Neisseria
69
Q

Buff

A

Diphteroids

70
Q

Brittle (forms splinters)

A

Nocardia spp.

71
Q

Creamy (butyrous)

A

Staphylococcus aureus

72
Q

Dry and Waxy

A

Diphteroids

73
Q

Sticky

A

Neisseria spp.

74
Q

Beaten egg white

A

Bacillus anthracis

75
Q
  • Convex
  • Entire edge
  • 2-3mm
  • Creamy, yellowish
  • Beta hemolytic
A

Staphylococcus

76
Q
  • Convex or pulvinate
  • Transluscent
  • Pinpoint
  • Butyrous
  • Wide zone of Beta hemolysis
A

Streptococcus

77
Q
  • Umbilicate or flat
  • Transluscent
  • Butyrous or mucoid
  • Broad zone of alpha hemolysis
A

Pneumococcus

78
Q
  • Pulvinate
  • semiopaque
  • gray
  • moist to dry
  • beta hemolysis may or may not be present
A

Escherichia coli and other Enterics

79
Q
  • Flat
  • gray
  • spreading as a thin film on agar
  • burnt chocolate
A

Proteus

80
Q
  • Flat
  • gray to greenish
  • margins erose or spreading
  • green-blue pigment
  • grapelike odor
A

Pseudomonas

MT6320 BACTE (13 decks)

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