Traditional Cultivation (Lab) Flashcards
Partial lysing of erythrocytes in a BAP around
and under the colony that results in a green
discoloration of the medium
alpha hemolysis
P. aeruginosa odor
fruitlike or grapelike
Pertains to Red blood Cells lysis or dissolution
Hemolysis
Loss of water =
increase solute concentration of media and bacterial lysis
Kluyvera spp pigment
blue
a type of culture that is inoculated directly from sample
PRIMARY
smells like freshly plowed field
Nocardia spp.
CFU Formula
CFU/mL= Number of colonies counted on the plate x Dilution Factor
organisms produce pink, dark pink, or red
colonies
Lactose Fermenter
bacteria that has a blue pigment
Kluyvera spp
cannot grow in the presence of O2, and the atmosphere in anaerobe jars,bags, or chambers
Anaerobes
grow in ambient air, which contains 21% O2 and a small amount of CO2
Aerobes
example of bacteria that exhibits partial lysing
Streptococcus pneumoniae
use of optimal artificial media and incubation
conditions to isolate and identify the bacterial
etiologies of an infection as rapidly and as
accurately as possible
CULTIVATION
increased concentrations of CO2 (5% to 10%) and approximately 15% O2
Capnophiles
Isolation technique is done To yield a ______ estimate of growth
semiquantitative
pH changes when lactose is ____
fermented
used to quantify colony forming units (CFU) in
urine cultures
Calibrated Inoculating Loop
Required temperature for bacteria
35-37 deg. celsius
Nocardia spp. smell
freshly plowed field
Reaction caused by enzymatic or toxin activity of Bacteria
Hemolysis
anaerobe but able to grow slowly and poorly in the presence of O2
Aerotolerant
a type of culture that is inoculated from the primary plate
Subculture
hemolysis of Streptococcus pyogenes
beta
musty basement, “mousy” or “mouse nest” smell
Haemophilus spp
under reduced O2 (5% to
10%) and increased CO2 (8% to 10%)
Microaerophiles
Haemophilus spp. smell
mousy
smells like an old sock
Staphylococcus aureus
bacteria that is brick-red especially at
room temperature
Serratia marcescens
Mincing of the tissue using sterile scissors and forceps into small pieces
Homogenization
Streaking technique for blunt slant media
Stab twice with ¾ deep of media; Do a streaking pattern upwards
smells fruity or grapelike
P. aeruginosa
Calibrated Inoculating Loop measurement
1 uL or 10 uL
P. aeruginosa pigment color
green, sometimes a metallic
sheen
S. aureus odor
old sock
inherent characteristic of a
specific organism confined generally to the
colony
pigmentation
Determined by touching the colony with a sterile loop
Consistency
has putrid smell
Proteus mirabilis
Increase atmospheric activity=
bacterial growth
How many times do you stab the butt media?
only once
