Traditional Cultivation (Lab)

Question 1

Partial lysing of erythrocytes in a BAP around
and under the colony that results in a green
discoloration of the medium

Answer 1

alpha hemolysis

Question 2

P. aeruginosa odor

Answer 2

fruitlike or grapelike

Question 3

Pertains to Red blood Cells lysis or dissolution

Answer 3

Hemolysis

Question 4

Loss of water =

Answer 4

increase solute concentration of media and bacterial lysis

Question 5

Kluyvera spp pigment

Answer 5

blue

Question 6

a type of culture that is inoculated directly from sample

Answer 6

PRIMARY

Question 7

smells like freshly plowed field

Answer 7

Nocardia spp.

Question 8

CFU Formula

Answer 8

CFU/mL= Number of colonies counted on the plate x Dilution Factor

Question 9

organisms produce pink, dark pink, or red
colonies

Answer 9

Lactose Fermenter

Question 10

bacteria that has a blue pigment

Answer 10

Kluyvera spp

Question 11

cannot grow in the presence of O2, and the atmosphere in anaerobe jars,bags, or chambers

Answer 11

Anaerobes

Question 12

grow in ambient air, which contains 21% O2 and a small amount of CO2

Answer 12

Aerobes

Question 13

example of bacteria that exhibits partial lysing

Answer 13

Streptococcus pneumoniae

Question 14

use of optimal artificial media and incubation
conditions to isolate and identify the bacterial
etiologies of an infection as rapidly and as
accurately as possible

Answer 14

CULTIVATION

Question 15

increased concentrations of CO2 (5% to 10%) and approximately 15% O2

Answer 15

Capnophiles

Question 16

Isolation technique is done To yield a ______ estimate of growth

Answer 16

semiquantitative

Question 17

pH changes when lactose is ____

Answer 17

fermented

Question 18

used to quantify colony forming units (CFU) in
urine cultures

Answer 18

Calibrated Inoculating Loop

Question 19

Required temperature for bacteria

Answer 19

35-37 deg. celsius

Question 20

Nocardia spp. smell

Answer 20

freshly plowed field

Question 21

Reaction caused by enzymatic or toxin activity of Bacteria

Answer 21

Hemolysis

Question 22

anaerobe but able to grow slowly and poorly in the presence of O2

Answer 22

Aerotolerant

Question 23

a type of culture that is inoculated from the primary plate

Answer 23

Subculture

Question 24

hemolysis of Streptococcus pyogenes

Answer 24

beta

Question 25

musty basement, “mousy” or “mouse nest” smell

Answer 25

Haemophilus spp

Question 26

under reduced O2 (5% to
10%) and increased CO2 (8% to 10%)

Answer 26

Microaerophiles

Question 27

Haemophilus spp. smell

Answer 27

mousy

Question 28

smells like an old sock

Answer 28

Staphylococcus aureus

Question 29

bacteria that is brick-red especially at
room temperature

Answer 29

Serratia marcescens

Question 30

Mincing of the tissue using sterile scissors and forceps into small pieces

Answer 30

Homogenization

Question 31

Streaking technique for blunt slant media

Answer 31

Stab twice with ¾ deep of media; Do a streaking pattern upwards

Question 32

smells fruity or grapelike

Answer 32

P. aeruginosa

Question 33

Calibrated Inoculating Loop measurement

Answer 33

1 uL or 10 uL

Question 34

P. aeruginosa pigment color

Answer 34

green, sometimes a metallic
sheen

Question 35

S. aureus odor

Answer 35

old sock

Question 36

inherent characteristic of a
specific organism confined generally to the
colony

Answer 36

pigmentation

Question 37

Determined by touching the colony with a sterile loop

Answer 37

Consistency

Question 38

has putrid smell

Answer 38

Proteus mirabilis

Question 39

Increase atmospheric activity=

Answer 39

bacterial growth

Question 40

How many times do you stab the butt media?

Answer 40

only once