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Bacteriology > Biosafety (LAB) > Flashcards

Biosafety (LAB) Flashcards

1
Q

process that kills all forms of microbial life, including bacterial endospores

A

Sterilization

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2
Q

What color of bin is use for Infectious waste/ pathological &
anatomical

A

Yellow

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3
Q

physical method of disinfection that Kills vegetative bacteria

A

Boiling at 100 deg Celsius in 15 minutes

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4
Q

BSC Class: In at front through HEPA to the outside or in to the room through HEPA

A

Class I

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5
Q

fire involves combustible cooking media such as oil

A

Class K

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6
Q

Involves flammable materials

A

Class B

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7
Q

Ideal and most common temperature control for AUTOCLAVE

A

15 psi pressure and 121°C for
30 minutes

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8
Q

Filtration of air

A

HEPA (High Efficiency Particulate Air)

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9
Q

What color of bin is use for radioactive waste

A

Orange

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10
Q

Disinfectants, kill endospores, toxic to human

A

Aldehydes-

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11
Q

higher temperatures than moist heat (160°Cto 180 °C); used to sterilize items such as glassware, oil,petrolatum, or powders

A

Dry Heat

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12
Q

removal of pathogenic microorganisms so items
are safe to handle or dispose of

A

Decontaimination

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13
Q

Dry heat temperature

A

160°C to 180 °C

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14
Q

Match the following bacteria to its biosafety level (BSL)

  1. HIV,Hep B, Salmonella
  2. Mycobacterium Tuberculosis
  3. : Bacillus subtilis and Naegleriagruberi
  4. Ebola virus, Chigo-Crimeanhemorrhagicfever
A
  1. BSL-2
  2. BSL_3
  3. BSL-1
  4. BSL-4
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15
Q

unlikely to be encountered in a routine clinical lab. Organisms are potentially infectious and transmitted through infectious aerosol

A

BSL-3

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16
Q

Disinfectants at high concentrations; used in soaps at low concentrations

A

Phenolics

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17
Q

requires longer exposure times (1.5to3hours)

A

Dry Heat

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18
Q

The important actions in case of fire and the order in which to perform tasks can be remembered with the
acronym RACE:

A
  1. Rescue any injuredindividuals.
  2. Activate the fire alarm.
  3. Contain (smother) the fire, if feasible (close fire doors).
  4. Extinguish the fire, if possible
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19
Q

a device that encloses a workspace in such a way as to protect workers from aerosol exposure to infectious disease agents

A

Biosafety Cabinet

20
Q

Filtration of liquid is done through the use of ____

A

cellulose acetate / cellulose nitrate membrane

21
Q

disinfectant; toxic at
high levels

A

Mercuric Chloride

22
Q

Steam under pressure; used to sterilize biohazardous trash and heat- stable objects

A

Moist Heat

23
Q

long wavelength and low energy

A

Non-ionizing radiation (UV)

24
Q

Decontamination of inanimate objects or surfaces; Preparation of patients for invasive procedures

A

Antiseptics

25
Q

Temperature and duration for Pasteurization

A

70 °C for 30 mins

26
Q

agents are those most commonly being sought
in clinical specimens and used in diagnostic, teaching, and
other laboratories

A

BSL-2

27
Q

kills food pathogens w/o damaging nutritional value or flavor

A

Pasteurization

28
Q

fire involves energized electrical equipment

A

Class C

29
Q

Supply air is HEPA-filtered. Exhaust air passes through two HEPA filters in series and is exhausted to the outside via a hard connection. Glove ports for attaching arm-length gloves to cabinet.

A

CLASS III

30
Q

fires involve combustible metals

A

Class D

31
Q

Involves combustible materials such as wood, paper, cloth, rubber, trash, and plastics

A

Class A

32
Q

Sterilization of heat-sensitive objects

A

Gases (Ethylene oxide)

33
Q

What color of bin is use for sharps

A

Red

34
Q

30% recirculated, 70% exhausted. Exhaust cabinet air must pass through a dedicated,internal cabinet duct to the outside through a HEPA filter

A

Class II, B

35
Q

Hazardous material burned to ashes at temperatures of 870°C to 980°C; Recommended and safest method

A

Incineration

36
Q

BSC: 70% recirculated to the cabinet work area through HEPA; 30% balance can be exhausted through HEPA back in to the room or to outside through a canopy unit

A

Class II, A

37
Q

disinfect drinking water; surface
disinfectants

A

Chlorine and chlorine
compounds-

38
Q

agents include those that
have no known potential for infecting healthy people and
are well defined and characterized.

A

BSL-1

39
Q

usually use as an eye drop solution.;for prophylactic treatment to prevent gonoccocal conjunctivitis in newborns

A

Silver Nitrate

40
Q

A process that destroys pathogenic organisms, but not necessarily all microorganisms, endospores, or prions

A

Disinfection

41
Q

method of choice for antibioticsolutions,toxic chemicals, radioisotopes, vaccines,and carbohydrates, which are all heat sensitive

A

Filtration

42
Q

What color of bin is use for Chemical Waste, Pharmaceutical waste

A

YELLOW W/
BLACK BAND

43
Q

Incineration temperature

A

870°C to 980°C

44
Q

designed to remove organisms larger than 0.3umfrom isolation rooms, operating rooms, and biologic safety cabinets (BSCs)

A

HEPA

45
Q

What color of bin is use for general/biological waste

A

Green

46
Q

agents are exotic agents that are highly contagious and cause life threatening disease. No vaccine or therapy available

A

BSL-4

Bacteriology (11 decks)

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