Biosafety (LAB) Flashcards
process that kills all forms of microbial life, including bacterial endospores
Sterilization
What color of bin is use for Infectious waste/ pathological &
anatomical
Yellow
physical method of disinfection that Kills vegetative bacteria
Boiling at 100 deg Celsius in 15 minutes
BSC Class: In at front through HEPA to the outside or in to the room through HEPA
Class I
fire involves combustible cooking media such as oil
Class K
Involves flammable materials
Class B
Ideal and most common temperature control for AUTOCLAVE
15 psi pressure and 121°C for
30 minutes
Filtration of air
HEPA (High Efficiency Particulate Air)
What color of bin is use for radioactive waste
Orange
Disinfectants, kill endospores, toxic to human
Aldehydes-
higher temperatures than moist heat (160°Cto 180 °C); used to sterilize items such as glassware, oil,petrolatum, or powders
Dry Heat
removal of pathogenic microorganisms so items
are safe to handle or dispose of
Decontaimination
Dry heat temperature
160°C to 180 °C
Match the following bacteria to its biosafety level (BSL)
- HIV,Hep B, Salmonella
- Mycobacterium Tuberculosis
- : Bacillus subtilis and Naegleriagruberi
- Ebola virus, Chigo-Crimeanhemorrhagicfever
- BSL-2
- BSL_3
- BSL-1
- BSL-4
unlikely to be encountered in a routine clinical lab. Organisms are potentially infectious and transmitted through infectious aerosol
BSL-3
Disinfectants at high concentrations; used in soaps at low concentrations
Phenolics
requires longer exposure times (1.5to3hours)
Dry Heat
The important actions in case of fire and the order in which to perform tasks can be remembered with the
acronym RACE:
- Rescue any injuredindividuals.
- Activate the fire alarm.
- Contain (smother) the fire, if feasible (close fire doors).
- Extinguish the fire, if possible
a device that encloses a workspace in such a way as to protect workers from aerosol exposure to infectious disease agents
Biosafety Cabinet
Filtration of liquid is done through the use of ____
cellulose acetate / cellulose nitrate membrane
disinfectant; toxic at
high levels
Mercuric Chloride
Steam under pressure; used to sterilize biohazardous trash and heat- stable objects
Moist Heat
long wavelength and low energy
Non-ionizing radiation (UV)
Decontamination of inanimate objects or surfaces; Preparation of patients for invasive procedures
Antiseptics
Temperature and duration for Pasteurization
70 °C for 30 mins
agents are those most commonly being sought
in clinical specimens and used in diagnostic, teaching, and
other laboratories
BSL-2
kills food pathogens w/o damaging nutritional value or flavor
Pasteurization
fire involves energized electrical equipment
Class C
Supply air is HEPA-filtered. Exhaust air passes through two HEPA filters in series and is exhausted to the outside via a hard connection. Glove ports for attaching arm-length gloves to cabinet.
CLASS III
fires involve combustible metals
Class D
Involves combustible materials such as wood, paper, cloth, rubber, trash, and plastics
Class A
Sterilization of heat-sensitive objects
Gases (Ethylene oxide)
What color of bin is use for sharps
Red
30% recirculated, 70% exhausted. Exhaust cabinet air must pass through a dedicated,internal cabinet duct to the outside through a HEPA filter
Class II, B
Hazardous material burned to ashes at temperatures of 870°C to 980°C; Recommended and safest method
Incineration
BSC: 70% recirculated to the cabinet work area through HEPA; 30% balance can be exhausted through HEPA back in to the room or to outside through a canopy unit
Class II, A
disinfect drinking water; surface
disinfectants
Chlorine and chlorine
compounds-
agents include those that
have no known potential for infecting healthy people and
are well defined and characterized.
BSL-1
usually use as an eye drop solution.;for prophylactic treatment to prevent gonoccocal conjunctivitis in newborns
Silver Nitrate
A process that destroys pathogenic organisms, but not necessarily all microorganisms, endospores, or prions
Disinfection
method of choice for antibioticsolutions,toxic chemicals, radioisotopes, vaccines,and carbohydrates, which are all heat sensitive
Filtration
What color of bin is use for Chemical Waste, Pharmaceutical waste
YELLOW W/
BLACK BAND
Incineration temperature
870°C to 980°C
designed to remove organisms larger than 0.3umfrom isolation rooms, operating rooms, and biologic safety cabinets (BSCs)
HEPA
What color of bin is use for general/biological waste
Green
agents are exotic agents that are highly contagious and cause life threatening disease. No vaccine or therapy available
BSL-4
