General media (LAB) Flashcards
MacConkey agar lactose fermenting colonies color
Pink
MTM
Modified Thayer- Thayer Martin
provides an indication of the type of organism
present based on oxygen requirements
SEMI-SOLID
MTM is selective for!l?
N. gonorrohoeae and N. meningiditis
Composed of soy broth
Thioglycollate broth
composed of mannitol as a primary carbon source
GN broth
pH indicator for MacConkey Agar
neutral red
GN
Gram Negative Broth
pH indicators for Hektoen enteric agar
bromthymol blue & acid fuchsin
Agarose need to be cooled between this temperature for distribution into petri dishes
55-60 deg. celsius
Non lactose fermenter color with HE agar
Green
Inhibits GN in XLD agar
Sodium deoxycholate
most common solidifying agent
Agarose
Cultivation of non-fastidious microorganism, determination of hemolytic reactions
Sheep Blood Agar
PEA
Phenylethyl Alcohol
Enrichment broth or semi-solid, supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms
Thioglycollate Broth
bacterial growth is indicated by turbidity
Broth media
Isolation and differentiation of Salmonella and Shigella spp. from other gram negative enteric bacilli with phenol red as the pH indicator
XLD
Blood agar based enriched with hemoglobin and supplement B
MTM
Components for BHI
Dextrose
Pork brain
Heart dehydrated infusion
MA
MacConkey Agar
Use to inhibit Proteus spp. in MTM agar
Trimethoprim
Agarose re-solidifies on this temperature
below 50 deg. celsius
composed pancreatic digest of casein
Thioglycollate broth
Selective for N.gonorrohoeae and N.meningitidis. Supports the growth of Francisella and Brucella spp.
MTM
Cultivation of non-fastidious
microorganism, determination of hemolytic
reactions
SHEEP BLOOD AGAR (BA)
BHI
Brain Heart Infusions
- needs are relatively complex, and exceptional
media components must be used for growth
fastidious
Peptones sources , 5%
defibrinated sheep blood
COLUMBIA CNA WITH BLOOD
Isolation and differentiation of lactose
fermenting and non–lactose- fermenting enteric bacilli
MACCONKEY AGAR
Selective isolation of gram-positive
cocci
COLUMBIA CNA WITH BLOOD
BA
Sheep Blood Agar
Selective isolation of aerobic gram-positive
cocci and bacilli
Phenylethyl Alcohol Agar
Components of Thioglycollate broth
Primary digest of casein
Glucose
Soy broth
MacConkey Agar non lactose fermenting colonies color
Colorless
- contain inhibitory agents against a specific bacteria,
while allowing only a “selected” bacteria to grow
SELECTIVE MEDIA
XLD
Xylose-Lysine-Deoxycholate
BA: Cultivation of non-fastidious microorganism, determination of ______
hemolytic reactions
a component of Columbia CNA w/ blood that blocks DNA replication
nalidixic acid
inhibits GN in PEA agar
phenylethyl alcohol
HE
Hektoen Enteric Agar
Vancomycin in MTM is use to?
Inhibit GP
Artificial environment where needs
of bacterial organisms are met to
proliferate and grow
MEDIA
Selective for N.gonorrohoeae and
N.meningitidis. Supports the growth of Francisella and
Brucella spp.
Modified Thayer-Thayer Martin Agar
pH indicator of XLD
Phenol red
CA
Chocolate Agar
h2s indicator
ferric ammonium citrate
supports growth of most non-fastidious organism
NUTRITIVE MEDIA
- used to enhance growth of a particular bacteria by
providing specific nutrients required
ENRICHMENT MEDIA
Agarose melts in this temperature
95 deg Celsius
Thioglycollate broth is use as an _____ broth or semi-solid, supports growth of ___,______,_____, and fastidious microorganisms
Enrichment
Anaerobes, aerobes, microaerophilic
Cultivation of fastidious microorganisms
such as Haemophilus spp., Brucella spp. and
pathogenic Neisseria spp
CHOCOLATE AGAR
A broth agar for cultivation of fastidious organisms
Brain Heart Infusion
H2s indicator in XLD
Ferric ammonium citrate
Nystatin in MTM is use to?
Inhibit yeast
Collistin in MTM is use to?
Inhibit GN
- disrupts GN cell membrane in Colombia CNA w/ blood agar
Colistin
Peptone base, enriched solution of
hemoglobin
CHOCOLATE AGAR
Made of Trypticase soy agar
BA
Selective (enrichment) liquid
medium for gastrointestinal pathogens
GRAM NEGATIVE (GN) BROTH
used to observe and detect the motility of
bacteria
SEMI-SOLID