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Bacteriology > General Practice Test > Flashcards

General Practice Test Flashcards

1
Q

product of transcription

A

mRNA strand

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2
Q

sterilization process that should be employed for laboratory culture medium

A

moist heat

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3
Q

product of replication

A

Two complementary DNA strands

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4
Q

True or False: The pentose phosphate pathway is the alternative to Entner-Doudoroff
pathway by some heterolactic bacteria like Brucella species

A

False (Its an alternative to EMP)

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5
Q

True or False: Substrate level phosphorylation involves the donation of phosphate from the substrate to ADP.

A

True

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6
Q

True or False: Entner-Doudoroff Pathway is a metabolic pathway utilize by oxidative
organisms to utilize glucose in the formation of ATP

A

true

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7
Q

precursor metabolite for initiating pentose phosphate pathway

A

Glucose

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8
Q

The spores of Bacillus subtilis var. globigii should be killed to determine the efficacy of which sterilant

A

Ethylene oxide

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9
Q

True or False: Alcohols are effective even in the presence of organic material.

A

False

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10
Q

True or False: Phenolics are components of germicidal soaps

A

True

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11
Q

Proposed the practice of washing hands with chlorinated lime solutions

A

Ignaz Philipp Semmelweis

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12
Q

True or False: In establishing the case definition, the microbiology laboratory must first isolate the only causative organism involve in the outbreak

A

True

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13
Q

true or False: An epidemiologic curve is an important visual aid in outbreak investigation

A

True

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14
Q

True or False: Previous unrecognized cases, even if they fit the case definition shall not be included in this outbreak if they did not occur at the time of the outbreak

A

false

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15
Q

How do we sterilize test tubes, volumetric pipets and glass slides in the laboratory

A

dry heat

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16
Q

It remains active in the presence of organic matter and does not coagulate
protein material.

A

glutaraldehyde/ peraceticacid

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17
Q

True or False: Clostridium perfringens is an organism whose toxins cause diarrhea
associated with health care infections.

A

False

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18
Q

true or False: Enterococci are pathogens of
concern in HAIs because of their possible resistance to vancomycin and their
potential ability to pass that resistance on to other bacteria.

A

True

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19
Q

True or False: mbulatory
care settings include a variety of different locations thus in these settings,
commonly encountered microorganisms of infection control importance also
varies.

A

False

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20
Q

bacteria that may employ glycolysis to convert metabolites

A

E. coli

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21
Q

True or False: The product of transduction is a recombination of the DNA of cells
previously infected by the phage.

A

True

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22
Q

True or False: : In plasmid conjugation, all of the plasmids
are transferred via the intercellular bridge

A

False (transferred through conjugative bridge)

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23
Q

Method of sterilization for HEPA filters

A

formaldehyde gas

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24
Q

Capable of self-replication

A

Episomes

25
Q

structure that is only present in Gram negative bacteria only

A

Porins

26
Q

S. epidermidis is a bacterium that is part of the skin’s microbiota. The host -
relationship with this organism can be described as

A

Symbiotic

27
Q

An intermediate metabolite in the two major metabolic pathways.

A

Pyruvate

28
Q

Common target of antimicrobial activity

A

Cell wall

29
Q

True or False: The origin of replication is an area determined by initiation proteins
containing approximately approximately 500 base pairs.

A

False (300 base pairs)

30
Q

True or False: An anticodon
consists of triplets of nucleotide bases that encodes for a specific amino acid for
binding with the mRNA

A

False (codon)

31
Q

True or False: Production of antisense strand is a method for silencing genes.

A

True

32
Q

final step in translation

A

ribosomal site A encounters UAG codon

33
Q

Certain bacteria are capable of colonization due to

A

Capsule

34
Q

True or False: S. aureus wound
infection of a diabetic person is a manifestation of an opportunistic infection.

A

True

35
Q

True or False: If person A is positive for the SARS-CoV-2 virus and is also symptomatic,
person A can be considered a carrier

A

False (Carriers Harbors the organism but do not present
symptoms to the disease)

36
Q

An acute carrier of S. typhi infection can live for years without manifesting the infection.

A

True

37
Q

first step in PCR

A

nucleic acid extraction

38
Q

Kanamycin is ineffective in this group of microorganisms.

A

Anaerobic Gram positive bacteria

39
Q

Does not induce MLSB methylase formation

A

Ketolides (telithromycin)

40
Q

Drugs not affected by resistance mechanisms that affect other drug classes

A

SXT

41
Q

Fluoroquinolones targets which enzyme in Gram negative

A

topoisomerases II

42
Q

A pivotal test in the identification schemes of Gram positive bacteria

A

Catalase test

43
Q

What is the purpose of phenol in AFB staining?

A

Phenol stains the AFB bacilli red.

44
Q

A key test in differentiating Pseudomonas species from Enterobacteriaceae
group.

A

Oxidase test

45
Q

C. acnes is a Gram positive anaerobe. What antibiotics would be effective for this microbe

A

Lincosamides (clindamycin and lincomycin)

46
Q

The drug binds to the cytoplasmic membrane and inserts its hydrophobic tail into
the membrane, disrupting the cell membrane and increasing its permeability,
specifically for Gram positive

A

daptomycin

47
Q

Its ability to disrupt cell membrane is also toxic to human cells.

A

polymyxin

48
Q

Mucoid colonies are associated with

A

capsule production

49
Q

Anaerobes requires an environment devoid of oxygen for growth. Anaerobes are
examples of

A

Non-fastidious bacteria

50
Q

The appearance of any species of Mycobacteria using a Kinyoun method of
staining:

A

Red bacilli against a blue background

51
Q

This is considered the differential step in Gram staining

A

Decolorization process with Acetone/Alcohol

52
Q

Only Pseudomonas aeruginosa may be resistant to the combined action of these
antimicrobial agents.

A

folic acid pathway inhibitor

53
Q

Why is vancomycin ineffective against Gram negative bacteria?

A

They are unable to penetrate the cell membrane of Gram negative bacteria due to their
large molecular size

54
Q

This is a type of antimicrobial employed considered more effective against
organisms that are more difficult to control in combination with the host’s
immune system.

A

Bactericidal

55
Q

Treatment for tuberculosis infection

A

RNA synthesis inhibitor

56
Q

Downregulation of bacterial DNA supercoiling in Gram negative bacteria

A

fluoroquinolones

57
Q

This antibiotic is highly active against a wide variety of gram-negative and grampositive bacteria; however, its use is limited becauseof its toxicity

A

Chloramphenicol

58
Q

20% sulfuric acid is an alternative decolorizer in which staining method?

A

Kinyoun stain

59
Q

Blocks the P-site in translation

A

linezolid

Bacteriology (11 decks)

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