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Bacteriology > Bacterial Identification > Flashcards

Bacterial Identification Flashcards

1
Q

binds to the nucleic acid either RNA or DNA and used to confirm the presence of bacteria in blood cultures when gram stain results are difficult to interpret or when the presence of bacteria is highly suspected and none are detected in light microscopy

A

Acridine Orange

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2
Q

Result is based on rapid production of bubbles or effervescence

A

Catalase test

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3
Q

Interpretation of primary cultures; a comparative examination of the colony
morphology of microorganisms growing on various culture media

A

Plate reading

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4
Q

Widal test is commonly use for

A

Salmonella typhi

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5
Q

It would nonspecifically bind to
nearly all Mycobacteria and would give the Mycobacterial cells a bright yellow appearance or orange against a greenish
background

A

Auramine Rhodamine

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6
Q

Amplification process where there is an Addition of other amino acids or nucleic acids into the sequence

A

Extension

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7
Q

Contain low concentrations of peptone and a single carbohydrate substrate such as glucose

A

O-F medium

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8
Q

a component of the cell wall that gives the ability to resist decolorization.

A

Mycolic Acid

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9
Q

An enzyme use to differentiate Staphylococcus from Streptococcus

A

Catalase

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10
Q

utilizes a method known as PCR

A

Amplification Methods

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11
Q

Enzyme being utilize for Oxidase test

A

Cytochrome oxidase

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12
Q

Special stain for flagella

A

Leifson Stain

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13
Q

O-F medium result: 1 tube has yellow top and green bottom and the other remain unchanged

A

Oxidizer

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14
Q

a term used to describe a particular genus in general.

A

Color

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15
Q

temp. required for Extension

A

72 deg. Celsius

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16
Q

A type of staining wherein instead of the organism itself, it’s the background that is
stained

A

Negative Stains

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17
Q

is one of the first method used to type strains of bacteria

A

Plasmid profile

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18
Q

One of the most common applications of this technique is the fluorescent treponemal antibody absorption test [FTA-ABS ] for your T. pallidum subspecies for detecting syphilis

A

Immunofluorescent Assays

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19
Q

detecting antigens by means of agglutination or clumping either with an artificial carrier
particle or insoluble matrix such as your latex bead and or with antibody bound to the
surface

A

Agglutination Assays

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20
Q

The basic method consists of antibodies bonded to enzymes instead of fluorochromes

A

Enzyme Immunoassays

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21
Q

pH indicator: Bromcressol purple

A

Purple to yellow

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22
Q

involves the ability of a
bacterial isolate to grow in the presence of one or
more inhibitory substances

A

Inhibitor profiles

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23
Q

pH indicator: pale yellow to pink

A

Andrade’s acid fuchsin-

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24
Q

Hard to grow in vitro, requires additional growth factors

A

Fastidious

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25
Q

an enzyme that mediates the extension process of amplification and is obtained from Thermus aquaticus.

A

Taq polymerase

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26
Q

specific PCR amplification product containing the
target nucleic acid

A

amplicon

27
Q

Based on the ability of two homologous nucleic acid strands to bond specifically with each other and form a double-stranded molecule (duplex or
hybrid)

A

Nucleic Acid Hybridization Methods

28
Q

O-F medium: Both tubes are yellow

A

Fermenter:

29
Q

an inherent characteristic of
a specific organism confined generally to the colony,

A

Pigmentation

30
Q

used to determine protein expression in disease conditions, such as cancers, genetic diseases, and
other diseases, including microbial infections.

A

Proteomics

31
Q

temp. required for annealing

A

50-58 deg. Celsius

32
Q

partial lysing of RBCs in an SBA (

A

α (alpha hemolysis)

33
Q

contain nutrients that support growth of most nonfastidious organisms without giving any particular organism a growth advantage

A

Nutritive media

34
Q

a technology that is used for rapidly identifying microorganisms, including fungi

A

MALDI-TOF Mass Spectrometry

35
Q

Pseudomonas spp.: Oxidative or fermenter?

A

Oxidative

36
Q

Positive result for acid

A

Yellow

37
Q

temp. required for denaturation

A

94 degrees Celsius

38
Q

Stain that is directed toward coloring specific components of the elements present

A

Differential Stain

39
Q

Grouping at the micron level of DNA molecules attached to a solid support

A

DNA Microarrays

40
Q

How should you determine the elevation?

A

The elevation should be determined by tilting the culture plate and looking at the side of the colony.

41
Q

a hazy blanket of growth on the surface that extends well beyond the streak lines

A

Swarming

42
Q

complete clearing of erythrocytes in SBA around
or under the colonies because of the complete
lysis of RBCs

A

β(beta hemolysis)

43
Q

identified based on susceptibility to optochin and solubility in bile

A

Streptococcus pneumoniae

44
Q

O-F medium: Both tubes does not change in color

A

Nonutilizer

45
Q

Can grow in the presence of various NaCl concentrations

A

Enterococci spp. and Vibrio spp.

46
Q

Enterobacteriacea: Oxidative or Fermenter?

A

Fermenter

47
Q

Stain that is directed toward coloring the forms and shapes present

A

Simple Stain

48
Q

identified based on Ability to hydrolyze esculin in the presence of bile

A

Enterococci spp.

49
Q

contain specific nutrients required for the growth of particular bacterial pathogens that maybe present alone or with other bacterial species in a patient specimen

A

Enrichment media

50
Q

Compare local strains to determine whether a local outbreak is caused by a single strain type or by multiple strains

A

Strain Typing

51
Q

Using sera to identify and differentiate bacteria

A

Serotyping

52
Q

contain one or more agents that are inhibitory to all organisms except those “selected”by the specific growth condition or chemical

A

Selective media

53
Q

FITC is conjugated directly to the specific antibody

A

Direct Fluorescent Antibody technique

54
Q

involves the use
of primers.

A

Annealing

55
Q

Reagent use for Oxidase Test

A

1% tetramethyl-p-phenylenediamine dihydrochrloride

56
Q

Determining the order of nucelotides in a fragment of DNA

A

Sequencing

57
Q

Acid fast stain method that utilizes heat

A

Ziehl-Neelsen

58
Q

Special stain for endospores;

A

Schaeffer-Fulton Stain

59
Q

identification is based on Ethanol survival

A

Bacillus spp.)

60
Q

alternative for acid alcohol as a decolorizer for AFB stain

A

20% Sulfuric Acid

61
Q

Assays involves the diffusion of soluble antigen and
antibody

A

Precipitation Assays

62
Q

Inoculation of the clinical specimen onto laboratory media

A

Primary Plating

63
Q

based on the ability of some bacteria and not others to utilize a substrate that often produces a change in the ph that is detected by a change in the color of a ph indicator

A

Differential media

64
Q

process of amplification method where it aims that the double stranded DNA would become single strands

A

Denaturation

Bacteriology (11 decks)

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