The Proper Technique of Mounting Stained Tissue Sections

Question 1

_____ and_____are some of the effects of oxidation on stained tissues

Answer 1

Bleaching and deterioration

Question 2

A______ medium is used to adhere the coverslip to the slide

Answer 2

mounting

Question 3

are available, which allow the mounting of tissues directly from the staining procedure

Answer 3

Aqueous based mounting media

Question 4

In aqueous mounting media

the water solubility of some stains allows them to bleed and/or fade in such mounting media, necessitating the use of…

Answer 4

resinous mounting media

Question 5

To use a ______mounting media, the section must first be dehydrated and cleared. This usually occurs during the last stages of staining.

Answer 5

nonaqueous or resinous

Question 6

Any water carried over to the mounting stage will show up as____ or ____, as the water droplets aggregate and distort the tissue.

Answer 6

bubbles or vacuole-like structures

Question 7

It is important to note also that the_____ agent used must be compatible with the mounting medium, or the sections must be thoroughly dried prior to mounting. Thus, a good knowledge of various mounting media is necessary for the successful execution of the technique.

Answer 7

clearing

Question 8

Types of Mounting Media

Answer 8

Aqueous media
Resinous mounting media

Question 9

Histological sections which need to be examined for any length of time or to be stored, must be mounted under a…

Answer 9

cover-slip

Question 10

Aqueous Media

Usually used for mounting material which is…

Answer 10

unstained

Question 11

These are used for mounting sections from distilled water when the stains would be decolorized or removed by alcohol and xylene, as would be the case with most of fat stains (such as in Sudan methods).

Answer 11

Aqueous media

Question 12

Aqueous media

Some stains, like_____, tend to diffuse into the medium after mounting.

This can be avoided by using______.

Aqueous mountains require addition of bacteriostatic agents such as (3) to prevent the growth of fungi.

Answer 12

methyl violet

Highman’s medium

phenol, crystal of thymol or sodium merthiolate

Question 13

A very useful medium for mounting sections for fluorescent microscopy

Answer 13

Apathy’s medium (1.52)

Question 14

Recommended for fat stains.

Answer 14

Farrant’s medium (1.43)

Question 15

An excellent routine mountant for fat stains.

Answer 15

Glycerine jelly (1.47)

Question 16

Recommended for metachromatic dyes especially methyl violet.

Answer 16

Highman’s medium (1.52)

Question 17

It is the most common mounting media used in routine processing.

Answer 17

Resinous mounting media

Question 18

Resinous media are made up of natural or synthetic resins dissolved in (3)

Answer 18

benzene, toluene or xylene.

Question 19

It is used as 60% natural resin by weight in xylene.

H.&E stained slides are fairly well preserved but basic aniline dyes tend to fade and prussian blue is slowly bleached.

Slides take few months to dry

Answer 19

Canada balsam - (1.52)

Question 20

Polystyrene resin dissolved in xylene as a 20% solution.

It is most commonly used.

Answer 20

D.P.X. (1.52)

Question 21

Criteria of An Ideal Mounting Media

1. Refractive index should be as close as possible to that of the glass which is
   ______.
2. It should not cause stain to…
3. It should not____ or appear granular on setting.
4. It should____ too quickly.
5. It should not cause tissue sections to____.
6. It should set____ and ____
7. It should be stable, not change in____ and ____
8. It should be miscible with____.

Answer 21

1.518

diffuse or fade.

crack

dry

shrink

dry and hard

color or pH

xylene