EMBEDDING

Question 1

Now that the specimen is thoroughly infiltrated with wax it must be formed into a “____” which can be clamped into a microtome for section cutting

Answer 1

block

Question 2

process by which tissues are surrounded by a medium such as agar, gelatin, or wax which when solidified will provide sufficient external support during sectioning.

Answer 2

Embedding

Question 3

Embedding is carried out using an “______” where a mould is filled with____ and the specimen placed into it.

Answer 3

embedding centre

molten wax

Question 4

The specimen is very carefully orientated in the mold because its placement will determine the “_____”, an important consideration in both diagnostic and research histology.

Answer 4

plane of section

Question 5

A______ is placed on top of the mould, topped up with more wax and the whole thing is placed on a_____ to solidify

Answer 5

cassette

cold plate

Question 6

When this is completed the block with its attached cassette can be removed from the mould and is ready for_____.

It should be noted that, if tissue processing is properly carried out, the tissue blocks are very stable and represent an important source of archival material.

Answer 6

microtomy

Question 7

is the process by which tissues are first embedded or fully infiltrated with a supporting medium such as____ or ____, then infiltrated a second time with____ in which they are also embedded.

Answer 7

Double embedding

agar or nitrocellulose

wax

Question 8

Embedding tissues in wax requires the use of molds that will help shape the finished product. The following are examples of embedding molds to use:

Answer 8

1. Plastic embedding rings and base molds
2. Compound embedding units
3. Leuckhart’s embedding mold
4. Disposable embedding molds

Question 9

: the rings may later serve as block holder during sectioning.

Answer 9

Plastic embedding rings and base molds

Question 10

: a containment with several compartments allowing the embedding of many specimen at a time, thereby reducing the time needed for blocking.

Answer 10

Compound embedding units

Question 11

: an adjustable mold made up of 2 L-shaped metal that can ba arranged on a flat metal plate.

Answer 11

Leuckhart’s embedding mold

Question 12

Disposable embedding molds: these include

Answer 12

peel-away types
plastic ice trays
paper boats