Test for Fecal Fat Flashcards
the presence of increase fats in stool
Steatorrhea
Steatorrhea Fecal characteristic
Greasy; foul odor; spongy consistency
Steatorrhea fecal volume
increased
Steatorrhea causes
Pancreatic insufficiency, Malabsorption, Maldigestion, and Absence of bile
inadequate intestinal absorption of processed foodstuffs despite normal digestive ability
Malabsorption
an inability to convert foodstuffs in the gastrointestinal tract into readily absorbable substances
Maldigestion
Stain for Triglycerides
NEUTRAL FAT STAIN
NEUTRAL FAT STAIN procedure
emulsified stool + 95% ETOH + Sudan III
Stain for total fat content (including Fatty acids, soaps/fatty acid salts, and cholesterol)
SPLIT FAT STAIN
SPLIT FAT STAIN procedure
stool + 36% acetic acid + Sudan III + Heat
Neutral Fat Stain NORMAL
Malabsorption
Neutral Fat Stain INCREASED
Maldigestion
Split Fat Stain
INCREASED
NORMAL
Malabsorption
Maldigestion
Gold standard quantitative test for fecal fat content
Van De Kamer Test
Collection Considerations for Van De Kamer Test
- Requires the collection of at least a 3 days specimen.
- The patient must maintain a regulated intake of fat (100 g/day) before and during the collection period
- The specimen is collected in a large, pre-weighed container like paint cans
- The entire fecal collection is weighed and homogenized (e.g., using a mechanical shaker).
Van De Kamer Test principle
Titration or titrimetric method
Van De Kamer Test reagent
Sodium hydroxide (naoh)
Formula for Fecal Fat:
Percent fat retention = [(Dietary fat - fecal fat) / Dietary fat] x 100
Values less than 95% indicate _____ in these individuals
steatorrhea
Disadvantage of VAN DE KAMER TEST
time consuming and uses corrosive and flammable solvents
this is useful for children when standard fat diet is used
VAN DE KAMER TEST
A test that is useful to differentiate malabsorption and maldigestion
D-XYLOSE TEST
______ is a pentose sugar that does not need to be digested but does need to be absorbed to be present in the urine.
D-Xylose
The xylose absorption test involves the patient’s ingestion of a dose of xylose, followed by the collection of a blood sample after ______________
2 hours and a 5-hour urine specimen.
If D-xylose result is low/abnormal, the result indicates a ________
malabsorption