CSF PROTEIN DETERMINATION Flashcards

1
Q

preferred method

A

Turbidimetric

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2
Q

T/F. Turbidimetric precipitates only albumin

A

FALSE. Turbidimetric precipitates both albumin and globulin

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3
Q

it precipitates albumin only:

A

Sulfosalicylic Acid (SFA)

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4
Q

to precipitate globulins, add

A

Sodium Sulfate

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5
Q

Dye binding uses

A

Coomasie Brilliant Blue

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6
Q

assess the integrity of the blood brain barrier

A

CSF /Serum Albumin Index

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7
Q

Index of 100

A

complete damage to BBB

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7
Q

CSF /Serum Albumin Index
Normal value:
Abnormal value:

A

Normal value: <9
Abnormal value: >9

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8
Q

assess condition with IgG production within the CNS (Multiple Sclerosis)

A

IgG Index

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9
Q

indicative of IgG production within the CNS

A

IgG Index

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10
Q

IgG Index
Normal value:
Abnormal value:

A

Normal value: <0.70
Abnormal value: >0.70

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11
Q

done in conjunction with serum electrophoresis

A

CSF electrophoresis

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12
Q

for the detection of oligoclonal bands

A

CSF electrophoresis

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13
Q

The presence of 2 or more oligoclonal bands in CSF but not in serum is valuable for the diagnosis of: (4)

A

o Multiple sclerosis
o Neoplastic disorders
o Encephalitis
o Guillain-Barre’ syndrome

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14
Q

protein component of the lipid protein complex that insulate the nerve fibers

A

Myelin Basic Protein

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15
Q

presence of MBP in CSF indicates destruction of _____

A

myelin sheath

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16
Q

used to monitor course of Multiple Sclerosis

A

Myelin Basic Protein

17
Q

_______ techniques are used for measurement in Myelin Basic Protein

A

Immunoassay techniques

18
Q

Done in conjunction with blood glucose

A

CSF Glucose

19
Q

Specimen for blood glucose should be drawn ____ prior to spinal tap

20
Q

CSF Glucose Normal values

A

60-70% of the plasma glucose

21
Q

CSF Glucose is increased

A

Due to increase plasma glucose (not significant)

22
Q

CSF Glucose is decreased in

A

Bacterial, Fungal, and Tubercular Meningitis

23
Q

CSF glucose is normal in

A

Viral Meningitis

24
CSF lactate levels can be a valuable aid in diagnosing and managing ____
meningitis
25
CSF lactate normal values
Normal value: 10-22 mg/dl (1.1 to 2.4 mmol/L)
26
T/F. CSF Lactate is directly proportional to glucose
CSF lactate is inversely proportional to glucose
27
Frequently CSF lactate levels are used to monitor patients with ______
severe head injuries
28
RBCs contain high concentrations of lactate, and results that are falsely elevated may be obtained on __________ or _______
xanthochromic or hemolyzed fluid
29
CSF Lactate Values Bacterial meningitis Fungal and tubercular meningitis Viral meningitis
Bacterial meningitis - Increased (>35mg/dl) Fungal and tubercular meningitis - Increased (>25mg/dl) Viral meningitis - Normal (remains lower than 25mg/dl)
30
Product of ammonia and alpha-ketoglutarate
CSF Glutamine
31
Indirect test for the presence of excess ammonia in CSF
CSF Glutamine
32
Why CSF Glutamine is preferred over direct measurement of CSF ammonia?
a because the glutamine concentration remains more stable than the volatile ammonia concentration in the collected specimen
33
CSF Glutamine Normal value
Normal value: 8 to 18 mg/dl
34
CSF Glutamine is increased in
Reye’s syndrome, Disturbance of consciousness/coma (more than 35 mg/dl)
35
Brain Tissue LD
LD1 and 2
36
Lymphocytes LD
LD2 and 3
37
Neutrophils LD
LD4 and 5
38
Serum LDH Normal = Flipped pattern =
Normal = LDH 2>1>3>4>5 Flipped pattern = LD1>2>3>4>5 (Acute Myocardial Infarction)
39
CSF LDH Normal = Neurological abnormalities= Bacterial meningitis=
Normal = LDH 2>1>3>4>5 Neurological abnormalities= LDH 1>2>3>4>5 Bacterial meningitis= LDH 5>4>3>2>1
40
Detects gram-negative bacterial endotoxin
Limulus Lysate Test
41
Limulus Lysate Test Reagent
Blood of horseshoe crab