CSF PROTEIN DETERMINATION

Question 1

preferred method

Answer 1

Turbidimetric

Question 2

T/F. Turbidimetric precipitates only albumin

Answer 2

FALSE. Turbidimetric precipitates both albumin and globulin

Question 3

it precipitates albumin only:

Answer 3

Sulfosalicylic Acid (SFA)

Question 4

to precipitate globulins, add

Answer 4

Sodium Sulfate

Question 5

Dye binding uses

Answer 5

Coomasie Brilliant Blue

Question 6

assess the integrity of the blood brain barrier

Answer 6

CSF /Serum Albumin Index

Question 7

Index of 100

Answer 7

complete damage to BBB

Question 7

CSF /Serum Albumin Index
Normal value:
Abnormal value:

Answer 7

Normal value: <9
Abnormal value: >9

Question 8

assess condition with IgG production within the CNS (Multiple Sclerosis)

Answer 8

IgG Index

Question 9

indicative of IgG production within the CNS

Answer 9

IgG Index

Question 10

IgG Index
Normal value:
Abnormal value:

Answer 10

Normal value: <0.70
Abnormal value: >0.70

Question 11

done in conjunction with serum electrophoresis

Answer 11

CSF electrophoresis

Question 12

for the detection of oligoclonal bands

Answer 12

CSF electrophoresis

Question 13

The presence of 2 or more oligoclonal bands in CSF but not in serum is valuable for the diagnosis of: (4)

Answer 13

o Multiple sclerosis
o Neoplastic disorders
o Encephalitis
o Guillain-Barre’ syndrome

Question 14

protein component of the lipid protein complex that insulate the nerve fibers

Answer 14

Myelin Basic Protein

Question 15

presence of MBP in CSF indicates destruction of _____

Answer 15

myelin sheath

Question 16

used to monitor course of Multiple Sclerosis

Answer 16

Myelin Basic Protein

Question 17

_______ techniques are used for measurement in Myelin Basic Protein

Answer 17

Immunoassay techniques

Question 18

Done in conjunction with blood glucose

Answer 18

CSF Glucose

Question 19

Specimen for blood glucose should be drawn ____ prior to spinal tap

Answer 19

2 hours

Question 20

CSF Glucose Normal values

Answer 20

60-70% of the plasma glucose

Question 21

CSF Glucose is increased

Answer 21

Due to increase plasma glucose (not significant)

Question 22

CSF Glucose is decreased in

Answer 22

Bacterial, Fungal, and Tubercular Meningitis

Question 23

CSF glucose is normal in

Answer 23

Viral Meningitis

Question 24

CSF lactate levels can be a valuable aid in diagnosing and managing ____

Answer 24

meningitis

Question 25

CSF lactate normal values

Answer 25

Normal value: 10-22 mg/dl (1.1 to 2.4 mmol/L)

Question 26

T/F. CSF Lactate is directly proportional to glucose

Answer 26

CSF lactate is inversely proportional to glucose

Question 27

Frequently CSF lactate levels are used to monitor patients with
______

Answer 27

severe head injuries

Question 28

RBCs contain high concentrations of lactate, and results that are falsely elevated may be obtained on __________ or _______

Answer 28

xanthochromic or hemolyzed fluid

Question 29

CSF Lactate Values
Bacterial meningitis
Fungal and tubercular meningitis
Viral meningitis

Answer 29

Bacterial meningitis - Increased (>35mg/dl)
Fungal and tubercular meningitis - Increased (>25mg/dl)
Viral meningitis - Normal (remains lower than 25mg/dl)

Question 30

Product of ammonia and alpha-ketoglutarate

Answer 30

CSF Glutamine

Question 31

Indirect test for the presence of excess ammonia in CSF

Answer 31

CSF Glutamine

Question 32

Why CSF Glutamine is preferred over direct measurement of CSF ammonia?

Answer 32

a because the glutamine concentration remains more stable than the volatile ammonia concentration in the collected specimen

Question 33

CSF Glutamine Normal value

Answer 33

Normal value: 8 to 18 mg/dl

Question 34

CSF Glutamine is increased in

Answer 34

Reye’s syndrome, Disturbance of consciousness/coma (more than 35 mg/dl)

Question 35

Brain Tissue LD

Answer 35

LD1 and 2

Question 36

Lymphocytes LD

Answer 36

LD2 and 3

Question 37

Neutrophils LD

Answer 37

LD4 and 5

Question 38

Serum LDH
Normal =
Flipped pattern =

Answer 38

Normal = LDH 2>1>3>4>5
Flipped pattern = LD1>2>3>4>5 (Acute Myocardial Infarction)

Question 39

CSF LDH
Normal =
Neurological abnormalities=
Bacterial meningitis=

Answer 39

Normal = LDH 2>1>3>4>5
Neurological abnormalities= LDH 1>2>3>4>5
Bacterial meningitis= LDH 5>4>3>2>1

Question 40

Detects gram-negative bacterial endotoxin

Answer 40

Limulus Lysate Test

Question 41

Limulus Lysate Test Reagent

Answer 41

Blood of horseshoe crab