CSF CELL COUNTING Flashcards
Why do we need to perform CSF cell count immediately?
because WBCS and RBCs will begin to lyse within 1 hour
40% to 50% WBC disintegrates within _______
2 hours
If the specimen is refrigerated, WBC lysis can be ______ significantly to approximately ______, but not _____
reduced; 15%; completely prevented
Undiluted specimen appearance
Clear
1:10 diluted specimen appearance
Slightly crazy eme hazy
1:20 diluted specimen appearance
HAZY
1:100 diluted specimen appearance
Slightly Cloudy
bloody 1:200 diluted specimen appearance
Cloudy/ Slightly
1:10,000 diluted specimen appearance
Bloody/Turbid
is WBC count routinely performed on CSF?
YEZ NA YEZ
WBC diluting fluid
(3% glacial acetic acid (HAc) with Methylene Blue
WBC count normal values
Adults:
Neonates:
- Adults = 0-5 WBCs/ ul
- Neonates = 0-30 WBCs/ul
WBC differential count is performed on a ______
stained smear (using Wright’s stain)
When the differential count is performed, ______ should be counted, classified, and reported in terms of _____
100 cells; percentage
When a differential cell count is performed using a cytospin slide or concentrated smear, the entire slide should be scanned first using ______
low-power objective (10×).
Normal WBC in CSF
Adult:
Neonate:
Adult: 70% lymphocytes, 30% monocytes Neonate: Up to 80% monocytes
RBC count can be calculated by
subtracting WBC count from Total Cell count
T/F. WBC and RBC count is routinely done
FALSE. Only WBC count is routinely done. RBC is not.
Done only in case of traumatic tap to correct Total Protein and WBC count
RBC count
term for the increase in number of normal cells in CSF
Pleocytosis
Subtract 1 WBC for every _____ RBC’s seen
700
Subtract ____ Total protein for every 10,000 RBCs/ul seen
8 mg/dl
Subtract 1 mg /dl of TP for every ____ RBCs/ul seen
1,200
