CSF CELL COUNTING

Question 1

Why do we need to perform CSF cell count immediately?

Answer 1

because WBCS and RBCs will begin to lyse within 1 hour

Question 2

40% to 50% WBC disintegrates within _______

Answer 2

2 hours

Question 3

If the specimen is refrigerated, WBC lysis can be ______ significantly to approximately ______, but not _____

Answer 3

reduced; 15%; completely prevented

Question 4

Undiluted specimen appearance

Answer 4

Clear

Question 5

1:10 diluted specimen appearance

Answer 5

Slightly crazy eme hazy

Question 6

1:20 diluted specimen appearance

Answer 6

HAZY

Question 7

1:100 diluted specimen appearance

Answer 7

Slightly Cloudy

Question 8

bloody 1:200 diluted specimen appearance

Answer 8

Cloudy/ Slightly

Question 9

1:10,000 diluted specimen appearance

Answer 9

Bloody/Turbid

Question 10

is WBC count routinely performed on CSF?

Answer 10

YEZ NA YEZ

Question 11

WBC diluting fluid

Answer 11

(3% glacial acetic acid (HAc) with Methylene Blue

Question 12

WBC count normal values
Adults:
Neonates:

Answer 12

• Adults = 0-5 WBCs/ ul
• Neonates = 0-30 WBCs/ul

Question 13

WBC differential count is performed on a ______

Answer 13

stained smear (using Wright’s stain)

Question 14

When the differential count is performed, ______ should be counted, classified, and reported in terms of _____

Answer 14

100 cells; percentage

Question 15

When a differential cell count is performed using a cytospin slide or concentrated smear, the entire slide should be scanned first using ______

Answer 15

low-power objective (10×).

Question 16

Normal WBC in CSF
Adult:
Neonate:

Answer 16

Adult: 70% lymphocytes, 30% monocytes Neonate: Up to 80% monocytes

Question 17

RBC count can be calculated by

Answer 17

subtracting WBC count from Total Cell count

Question 17

T/F. WBC and RBC count is routinely done

Answer 17

FALSE. Only WBC count is routinely done. RBC is not.

Question 18

Done only in case of traumatic tap to correct Total Protein and WBC count

Answer 18

RBC count

Question 19

term for the increase in number of normal cells in CSF

Answer 19

Pleocytosis

Question 20

Subtract 1 WBC for every _____ RBC’s seen

Answer 20

700

Question 21

Subtract ____ Total protein for every 10,000 RBCs/ul seen

Answer 21

8 mg/dl

Question 22

Subtract 1 mg /dl of TP for every ____ RBCs/ul seen

Answer 22

1,200