APT TEST/ ALKALI DENATURATION TEST/ DOWNEY TEST

Question 1

• A test for Fetal hemoglobin
• Used to Differentiate fetal blood from maternal blood
• Discovered by Leonard Apt

Answer 1

APT TEST/ ALKALI DENATURATION TEST/ DOWNEY TEST

Question 2

APT TEST/ ALKALI DENATURATION TEST/ DOWNEY TEST SPECIMEN

Answer 2

infant stool, vomitus, emesis, or gastric aspirate

Question 3

APT TEST/ ALKALI DENATURATION TEST/ DOWNEY TEST REAGENT

Answer 3

1% NaOH

Question 4

APT TEST/ ALKALI DENATURATION TEST/ DOWNEY TEST PROCEDURE

Answer 4

o Emulsify specimen in water.
o Centrifuge.
o Divide pink supernatant into two tubes.
o Add 1% sodium hydroxide to one tube.
o Wait 2 minutes.
o Compare color with that in the control tube.
o Prepare controls using cord blood and adult blood

Question 5

APT TEST/ ALKALI DENATURATION TEST/ DOWNEY TEST Pink supernatant

Answer 5

fetal blood with Hemoglobin F

Question 6

APT TEST/ ALKALI DENATURATION TEST/ DOWNEY TEST Yellow Brown supernatant

Answer 6

maternal blood with Hb A