TCA Cycle Flashcards
What vitamin is succinate dehydrogenase derived from
Riboflavin
FAD and FMN are coenzymes for
Succinate dehydrogenase
What is a cofactor for pyruvate dehydrogenase
Thiamine pyrophosphate (B1)
What is the nicotinamide ring on NAD+?NADH derived from
Niacin B3
CoA is derived from
Pantothenic acid
Cofactor for pyruvate carboxylase
Biotin B7
What does pyruvate carboxylase do
Converts pyruvate to oxaloacetate when cycle is low on components
Pyruvate to acetyl CoA
Pyruvate dehydrogenase
Pyruvate to oxaloacetate
Pyruvate carboxylase
What enzyme is embedded in the mitochondrial membrane?
Succinate dehydrogenase
What complex is succinate dehydrogenase in
Complex II of ETC
What is the thioester bond in succinyl coA cleaved by
Succinate thiokinase
What is succinate thiokinase used for
Synthesis of high energy phosphate bond for GTP
What is in the a-keto acid dehydrogenase complex protein family
a-ketogluturate dehydrogenase complex
Pyruvate dehydrogenase complex
Branched chain amino acid dehydrogenase complex
Sources for acetyl coA
fatty acids (palmitate)
ketone body (acetoacetate)
Sugar (glucose)
Pyruvate
Alanine
Ethanol
E1
a-keto acid decarboxylase
Contains TPP
Cleaves off Co2
E2
Transacyclase containing lipoic acid
Transfers acyl of a-keto acid from thiamine–>CoASH–> produces acetyl coA
E3
Dihydrolipoyl dehydrogenase contains FAD—> reduces it to NAD+—> generates NADH
Succinate dehydrogenase is derived from
B2 Riboflavin
Pyruvate dehydrogenase and a-ketogluatare dehydrogenase contain
TPP B1
What happens to the lipoate on E2 in pyruvate dehydrogenase
It is reduced when it accepts the acyl group from E1
Which vitamins are necessary for decarboxylation of a-ketoglutarate
B1, 2, 3, 5
What is derived from coenzyme A
B5 pantothenic acid
Which enzyme involves TPP (B1)
a-ketoglutarate dehydrogenase
Lipoate is attached to what
e-amino group of lysine of transacylase enzyme
What form is transylase enzyme
E2
What happens to the oxidized lipoate disulfide form
Reduced, accepts acyl group from TPP
Arsenic poisioning
Lipoic acid is a target of arsenite inhibition due to binding of -SH groups
Which enzymes produce NAD+/NAH
Malate dehydrogenase, a-ketoglutarate, isocitrate dehydrogenase
What is a-ketoglutarate dehydrogenase derived from
TPP
Which part of the TCA is positive delta G
Citrate to isocitrate
Malate to OAA
What is citrate synthase controlled by
Concentration of AA, substrate, and citrate
What is citrate an inhibitor of
Product inhibitor, Its competitive with OAA
Isocitrate dehydrogenase is activated by ____ during muscular contraction
Ca++
Isocitrate dehydrogenase is allosterically activated by
ADP