Storage and Expression of Genetic Material

Question 1

pyrimidines

Answer 1

cytosine, thymine, uracil ( CUT the py )

Question 2

responsible for the negative charge of DNA and RNA

Answer 2

phosphate groups

Question 3

enzymes necessary for denovo synthesis of purines are found

Answer 3

cytoplasm

Question 4

pathway used for ribose-5-phosphate synthesis

Answer 4

HMP pathway

Question 5

amino acids used in purine synthesis

Answer 5

aspartate, glycine, glutamine

Question 6

besides amino acids, what is needed for purine synthesis

Answer 6

N10-formyl-tetrahydrofolate & CO2

Question 7

converts ribose-5-phosphate to PRPP

Answer 7

ribose phosphate pyrophosphokinase/ PRPP synthase

Question 8

activates PRPP synthase

Answer 8

inorganic phosphate

Question 9

rate-limiting step of purine synthesis

Answer 9

PRPP to 5-phosphoribosylamine via glutamine: PRPP aminotransferase

Question 10

inhibitors of glutamine: PRPP aminotranserase

Answer 10

AMP, GMP, IMP (purine nucleotides)

Question 11

activators of rate-limiting enzyme of purine synthesis

Answer 11

PRPP & Glutamine

Question 12

parent purine. ( first one made )

Answer 12

IMP

Question 13

Purine denovo synthesis requires how many ATP

Answer 13

4

Question 14

methotrexate inhibits

Answer 14

dihydrofolate reductase ( converts folic acid to THF )

Question 15

drug that inhibits THF synthesis in prokaryotes

Answer 15

trimethoprim

Question 16

lack of folic acid leads to

Answer 16

megaloblastic anemia

Question 17

folate analogue used in chemotherapy

Answer 17

methotrexate and aminopterin. ( kills all rapidly dividing cells )

Question 18

Synthesis of AMP requires

Answer 18

GTP

Question 19

Synthesis of GMP requires

Answer 19

ATP

Question 20

mycophenolic acid inhibits

Answer 20

IMP dehydrogenase

Question 21

PABA analogs that competitively inhibit bacterial synthesis of folic acid

Answer 21

sulfonamides

Question 22

using the enzyme ________, hypoxanthine is converted to _______ in the purine salvage pathway

Answer 22

HGPRT, IMP

Question 23

using the enzyme ________, Guanine is converted to _______ in the purine salvage pathway

Answer 23

HGPRT, GMP

Question 24

in the purine salvage pathway, adenine is converted to AMP via

Answer 24

APRT

Question 25

disease associated with deficient HGPRT

Answer 25

Lesch-Nyhan syndrome

Question 26

Lesch-Nyhan syndrome presents with

Answer 26

hyperuricemia (excess uric acid production) and Gout

Question 27

genetic inheritance of Lesch-Nyhan

Answer 27

X-linked recessive

Question 28

infants with Lesch-Nyhan syndrome present with

Answer 28

orange crystals in Huggies

Question 29

treatment of Lesch-Nyhan

Answer 29

allopurinol

Question 30

Cofactor for rate limiting step of purine synthesis

Answer 30

Mg

Question 31

Inhibits IMP dehydrogenase

Answer 31

Mycophenolic acid