Sterilisation Methods + Instrument Cleaning (+CR) Flashcards
Why can’t boiling be considered an adequate method of sterilization?
Does not reach a high enough temperature to destroy bacterial spores
State the 7 steps used to clean endotracheal tubes
1. Rinse in running water
(Any debris + fluid could deactivate detergent)
2. Soak in detergent
(Soften residual debris)
3. Scrub inside + out with brush
(Remove all debris + mucus, lumen must maintain clean + patent)
4. Rinse thoroughly
(All detergent must be removed, to prevent chemical + ischaemic tracheitis)
5. Dry thoroughly + check cuff inflation + for any wear and tear
(Faulty tubes can comprimise anaesthetic + increase morbidity)
6. Sterilize - Rubber = Ethylene Oxide - air for 48hrs + Silicone = Autoclave
(Heat damages rubber tubes = cannot be autoclaved. EO sterilization requires airing to avoid chemical tracheitis)
7. Store in dry, cool + enclosed environment, out of direct sunlight
(Correct care + storage = prolong life)
What two chemical solutions can be considered as a method of disinfection?
Chlorhexidine
or
Glutaraldehyde
What type of heat does a hot-air oven produce?
Dry heat
Are microorganisms more resistant to dry heat?
Yes
If dry heat was to be used as a method of sterilization, what must be considered?
That the instrument needing sterilized, is subjected to dry heat for a long period of time
If dry requires long periods of time to be sterilized, then how long of a cooling period is required?
A longer cooling period
What are chemical indicator strips?
- Paper strips
- Changes colour when the correct time + temp has been reached + maintained
What method of sterilization do you use chemical indicator strips for?
The Autoclave
(Ethylene Oxide)
What is a Browne’s tube?
- Small glass tubes
- Filled with an orange liquid
- Turns green when the correct time + temp has been reached + maintained
What method of sterlization are Browne’s tubes used for?
Autoclave
+
Hot-air
What is Bowie-Dick indicator tape?
- A beige tape
- Impregnated with chemical stripes
- Changes to black when a temp of 121 degrees celsius
- Does not indicate that the pack has been exposed for the correct amount of time
What method of sterlization is Bowie-Dick indicator tape used for?
Autoclave
What is Ethylene Oxide tape?
Same as Bowie-Dick indicator tape, but the tape is green
+
Lines change red, when exposed to EO
What method of sterlization is Ethylene Oxide tape used for?
Ethylene Oxide
What are spore strips?
- Strips of paper
- Impregnanted with spores - Bacillus stearothermophilius
- After sterilization - culutred for 72hrs
- If no sterilization has been achieved - no growth will be visable
- Accurate
What is the disadvantage of Spore strips?
Despite the accuracy, there is a long delay in awaiting results
What are the 3 methods of sterlization can Spore strips be used for?
- Autoclave
- Ethylene Oxide
- Hot-air oven
What are Thermocouples?
- Electrical leads with temp-sensitive tips
- Placed in autoclave, with leads passed out + attached to a recording device
- Temp is checked throughout the cycle
- Results recorded
What method of sterlization is Thermocouples used for?
Autoclave
What are the advantages of Self-seal pouches for packaging materials?
- Easy to pack
- Clear front - easy visablility
- Paper back + sterilization indicator
- Ideal for individual instruments
What are the 2 main disadvantages of Self-seal pouches for packaging materials?
- Can be punctured easily by heavy or sharp instruments
- May have to use double packaing to further prevent puncturing - but increases costs
What 2 methods of sterilization are suitable for Self-seal pouches?
- Autoclave
- Ethylene Oxide