starting Flashcards
1
Q
how to isolate a protein
A
take gene of interest insert via a plasmid -> transfect the cell -> host exspansion in bioreactor -> protein purification
2
Q
name two digestive proteins
A
trypsin
HIV protease
3
Q
What are three methods to identify protein structure
A
protein crystalography
cryo-electron microscopy
NMR spectroscopy
4
Q
name 3 Metabolisum linked proteins + function
A
alcohol dehydrogenase - metabolize ethaol
hexokinase - binds phosphate groups to glucose
