protein design

Question 1

What are the two factors for recombinent technologys

Answer 1

Finantial - cost + speed
Biomechincal - post translational modification + protein folding

Question 2

What is the application for designing proteins

Answer 2

Industreal
Drug development
reserch
agricultral
theriputics

Question 3

What are the steps to design a new proteindirected

Answer 3

1. identify a need - what do you want to imporove
2. select target protein - what protein does this
3. design protein - what structural changes are needed to imporove -> shape = function
4. gene synthesis - directed evolution, changing specific nucleotides
5. exspression + purification - use recombinant techneques to replicate + make protein
6. characterisation + optimization - define protein + ensure it performs to its best

Question 4

What is gene therapy + example of how this is used medically

Answer 4

Gene therapy is using recombiant techniques to insert a vector into a human to enable transcription of a desired protein
- makes human = exspression system
uses viral envelope to get gene into specific cell
insulin to combate T2 diabetes
use insulin genes to imput them into pancreatic alpha cells to create production

Question 5

What is an agricutral example of biotechnology

Answer 5

use transgenic plants to create proteins -> casein
- help feed population growth

make transgenic plant with casein gene -> grow plant -> extract speeds with protein exspressed -> purify