Lipid as fuel 2 Flashcards
What are the two methods which lipids can be transported to tissues
Diet + Liver -> TAG breakdown by LPL + transported in VLDL + Chylomicrons
Adipose -> adipose lipase broken to 3 FFA + glycerol
glycerol -> liver
FFA bind to albumin in hydrophobic pocket -> Albumin-FFA to travel in blood
Transmembrane transport of FFA
bind to FABP -> FABP-FFA in tissue
What activation of FFA occurs in the cytosol
FFA + CoASH + ATP -> fatty acyl-CoA + AMP
- Burns 2 atp equivlants to bind CoA to molecule
How does fatty acyl-CoA get transported from the cytosol to the mitochonral matrix
outter membrane -> fatty acyl-CoA tranporter protein
Inner membrane -> fatty acyl-carnitine transporter protein
Intermembrane space- fatty acyl-CoA -> fatty acyl-carnitine, in a reversable reaction by carnitine acyltransferase 1 on outter membrane
Mitochondral matrix - fatty acylcarnitine -> fatty acyl-CoA, reverse reaction by carnitine acyltransferase 2 inner membrane
What are the general prinicapls/requirements around B oxidation of fatty acids
Requires an even C length + saturated fatty acid - occuring in matrix
Produces no ATP just NADH + FADH2
Cycle process where acyl chain reused 2 carbons shorter each loop
repeates ((n/2)-1) times
What are the 4 steps of beta oxidation
- oxidation: 2H reduced by FAD+->FADH2
- hydration: Formation of OH across double bond
- oxidation: ketone formation at OH reduction NAD->NADH
- clevage: 2 end C cleved to form acytl-CoA, CoA attached to remaining C chain
What is the function of the 4 steps of beta oxidation
steps 1-3: Rearangement aroudn the bond for clevage + energy capture
step 4: Clevage of the bond -> releasing Actyl-CoA for further oxidation in CAC + fatty acyl-CoA reproduction (2C shorter)
What is the overall reaction for beta oxidation
n-chain -CoA + n/2-1 (NAD+ + FAD+ + H2O + CoASH) -> n/2 Actyl-CoA + n/2-1 (NADH/H+ + FADH2)
+ heat as overall spontaneous reaction
What is the properties of FADH
Ribofalvin B2 dirrivative
- Redox, 2 reduction potentions
- Strong binding to 2H+
