Stallion BSE Flashcards
when do colts hit puberty?
8-30 months
67% of mature size
100 mil sperm/ejac
T/F - libido is seasonal in stallions?
False
How long is the complete spermiogenesis cycle?
57 days+transport so 71 days total
the caput is — and faces
head of the epididymus and faces cranially
Which internal genitalia are palpable?
ampulla
what gives the preseminal fraction?
the Bulbourethral glands
what gives acidic secretions?
prostate
what gives the gel fraction?
seminal vesicles
what does the ampulla do?
sperm storage and secretions
WHat is the lowest PR we should accept from a stallion?
30%
Seasonal PR should be above?
85%
Average PR % per cycle
45-55
WHen to start stallion BSE
2 years or over
what do we look at for stallion BSE?
testicles - consistent, size, position, ejac
daily sperm to test mass/volume
reasons for a stallions BSE
pre-purchase plan mares to be bred CTS for AI or something post-injury when not successful
What degree of sampling for the stallion BSE?
2 collections and evaluations 1 hour apart.
what is acceptable total scrotal width?
> 10 cm
preparing the stallion procedure
tease a bit, look at penis, culture any masses or things from the urethra fossa, wash the penis, mount and collect
preparing the AV for collection
non-spermicidal lube on the ring, put on collection bag, fill with water to 55C (so 44-50 when sampling), mount onto phantom and divert penis when riding
what temperature does the AV need to be?
55 C (44-50 when sampling)
routine semen evaluation includes
removing water –> put through the filter –> measure gel-free volume –> evaluate progressive motility on 400X –> measure % progressive (speed) –> then concentration –> cacl total motile sperm –> sperm morphology (live/dead count) on 1000X –> total PMMNs
how do we dilute sperm with extender?
usually 1:1
what ratio of stain to sperm for morphology
60:40 on warm, dry slide.
What are possibilities when we see urosperia
HyPP
RAO
What do we classify as below average sperm?
50 mil/ml,
What do we classify as average sperm?
30-70% PMMNs, concentraion 50-100 mil/ml, 30-50% normal morph
What do we classify as above average sperm?
> 70% PMMNs, concentraion >100 mil/ml, >50% normal morph
What are some other good tests to do if things are abnormal?
Advanced Sperm Cell Analysis/lmaging Genetic tests (HYPP, HERDA, CID) Serum or semen for EVA Urethroscopy Ultrasonography Epididymal aspiration Testicular Biopsy
Hormonal analysis
