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Journals ECVS24 > Stains and IHC > Flashcards

Stains and IHC Flashcards

1
Q

IHC marker for:
- Carcinoma

A

Cytokeratin

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2
Q

IHC marker for:
- Sarcoma

A

Vimentin
Same as melanoma, mesothelioma

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3
Q

IHC marker for:
- Melanoma

A

Ki67
Proliferating cell nuclear antigen proliferation markers
Vimentin - (same as sarcoma, mesothelioma)
Melan-A

Neuron-specific enolase (if amelanotic)

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4
Q

IHC marker for:
- Rhabdomyosarcoma

A

Desmin

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5
Q

IHC marker for:
- Leimyosarcoma

A

Desmin

Smooth muscle actin

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6
Q

IHC marker for:
- T-cell lymphoma

A

CD3

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7
Q

IHC marker for:
- B-cell lymphoma

A

CD73a

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8
Q

IHC marker for:
- GIST

A

CD117 (KIT)
Same as MCT

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9
Q

IHC marker for:
- Schwannoma (STS)

A

Melan-A

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10
Q

IHC marker for:
- MCT

A

CD117 (KIT) - same as GIST. Ass. w. tumour grade, recurrence and death
PCNA
Ki-67 - prognostic for reduced survival and increased local recurrence

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11
Q

IHC marker for:
- amelanotic melanoma

A

Neuron-specific enolase - same as neuroendocrine tumours

S-100

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12
Q

IHC marker for :
- neuronal/neuroendocrine

A

Neuron-specific enolase - same as amelanotic melanoma

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13
Q

IHC marker for:
- Haemangiosarcoma

A

Factor VIII-related Ag (vWF)

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14
Q

IHC marker for:
- Histiocytic tumour

A

CD-18
Lysozyme

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15
Q

IHC marker for:
- Ovarian granulosa cell tumours

A

Alpha-inhibins

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16
Q

IHC marker for:
- Mammary tumours

A

Ki-67 - prognostic for reduced survival and increased local recurrence
p53
HER 2 - correlated with COX-2 expression (worse prognosis)
BRCA
RAD51
VEGF
CD44
Phosphase and Tensin

17
Q

IHC marker for:
- Mesothelioma

A

Cytokeratin
Vimentin IHC

18
Q

Stain for:
- Osteosarcoma

19
Q

Stain for:
- Chylothorax

A

Sudan Black

20
Q

Stain for:
- Saliva/mucin

A

Periodic Acid Shiff

21
Q

Stain for:
- Liposarcoma

22
Q

Stain for:
- MCT

A

Romanowsky Stains (Diff-Quick, Hemacolour, May-Grunwald-Giemsa)
Toluidine blue

23
Q

What are AgNORs?

What does their presence indicate?

How are they stained?

A

Argyrophilic nucleolar organiser regions
AgNORs are present in MCT and mammary carcinoma

Increased mortality, local recurrence and decreased survival

Silver-based stains

24
Q

Stain for:
- Mycoplasmal organisms

A

Giemsa, Wright or Leishmann stains

25
Q

Stain for:
- Starch granules

A

Iodine staining
Polarised light microscopic examination

26
Q

Stain for:
- Mycobacetrium

A

Ziehl-Neelsen (acid fast stain)

27
Q

Stain for:
- fungal arthritis

A

Sabourauds agar culture

28
Q

Test for fungal osteomyelitis

A

Latex-agglutination testing for antigen - cryptococcosis
Serum antigen detection - blastomycosis and coccidioidomycosis

29
Q

Test for Neospora/Toxoplasma

A

Serum antibody titres

30
Q

Test for uroabdomen

A

Fluid : serum creatinine - >2:1
Fluid : serum potassium - >1.5x (dogs), >1.9x (cats)

31
Q

Test for bile

A

Fluid : serum bilirubin - >2:1
Basophilic mucinous sediment
Halls stain

Ictotest is inaccurate

32
Q

Diagnosing septic peritonitis:
- fluid analysis
- chemical analysis
- cytology

A

Fluid analysis:
- TP >3.0
- Cell count 5000-50,000+ cells/uL
- SG >1.013

Chemical analysis:
- Glucose <2.78 mmol/L (<50mg/dL)
- Blood-to-fluid glucose difference >1.12 mmol/L (>20mg/dL)

Cytology:
- Toxic and degenerative neutrophils
+/- intracellular bacteria/vegetable matter

33
Q

Diagnosing aseptic peritonitis:
- fluid analysis
- chemical analysis
- cytology

A

Fluid analysis (similar to septic):
- TP >3.0
- Cell count >5000 cells/uL
- SG >1.013

Chemical analysis:
- Glucose >2.78 mmol/L (>50mg/dL)

Cytology:
- Nondegenerative neutrophils
- No bacteria/vegetable matter
+/- crystals (starch)

34
Q

Diagnosing chyloperitoneum:
- fluid analysis
- chemical analysis
- cytology

A

Gross appearance - milky pink/creamy white, opaque

Fluid analysis:
- cell count 1000-17000 cells/uL

Chemical analysis:
- Cholesterol - fluid < serum
- Triglycerides - fluid > 3x serum

Cytology:
- Nondegenerative neutrophils
- Lymphocytes, macrophages, mesothelial cells

35
Q

IHC for Pheochromocytoma

A

Chromogranin A and Synaptophysin

37
Q

IHC for differentiation between benign and malignant tumours of perianal gland

A

PCNA (proliferating cell nuclear antigen)
Ki-67
AgNORs

Journals ECVS24 (19 decks)

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