Spectrophotometry

Question 1

intensity of radiant energy transmitted, reflected or emitted is related to concentration of chemical species that absorbs energy

Answer 1

Spectrophotometry

Question 2

Measurements of Spectrometry

Answer 2

Measurement of the position/wavelength in the electromagnetic spectrum where radiant energy has interacted with chemical species.
Measurement of the power of transmitter, emitted, reflected, or fluoresced energy.

Question 3

The complete system if energy propagated in wave form

Answer 3

Electromagnetic Spectrum

Question 4

Form of energy that is transmitted through space and energies; energy in wave form

Answer 4

Radiant Energy

Question 5

Radiant Energy

Answer 5

Electromagnetic Radiant

Question 6

Radiant Energy is energy in

Answer 6

UV Region
Visibile Region
Infrared Region

Question 7

Length of a complete wave or cycle, from the peak of one wave to the peak of the next

Answer 7

Wavelength

Question 7

Length of a complete wave or cycle, from the peak of one wave to the peak of the next

Answer 7

Wavelength

Question 8

Wavelength Range of Ultraviolet Region

Answer 8

200 - 380 nm ⭐

Question 9

Wavelength Range of Visible Region

Answer 9

380 - 780 nm

Question 10

Wavelength Range of Near Infrared Region

Answer 10

780 - 3000 nm

Question 10

Wavelength Range of Near Infrared Region

Answer 10

780 - 3000nm

Question 11

Wavelength Range of medium infrared Region

Answer 11

3 -15mm ⭐

Question 12

Wavelength Range of Far infrared Region

Answer 12

15 - 300mm

Question 13

Units of wavelength measurement ⭐

Answer 13

Nanometer
Micrometer
Angstrong

Question 14

Number of waves per centimeter and is equal to 1/wavelength (cm)

Answer 14

Wave number

Question 15

Number of complete cycles which pass a given point per second

Answer 15

Frequency

Question 16

A molecule containing chromophore

Answer 16

Chromogen

Question 17

A functional group which absorb radiant energy in the UV or visible regions of the spectrum

Answer 17

Chomophore

Question 18

Examples of Chromophore

Answer 18

Organic Acids
Aldehydes
Ketone
Acetylene
Ethylene
Azomethines

Question 19

Enhance absorption by a chromophore and shift the wavelength of absorption, when attached to the chromophore

Answer 19

Auxochrome

Question 20

Possess one or more unshared or lone pair of electron that interacts with other e- in chromophore

Answer 20

Auxochrome

Question 21

Branch of spectrometery whhch embraces the measurement of the absorption

Answer 21

Spectrophotometry

Question 22

Designate instruments which have a radiant energy-dispersing device, such as prism or grating, permiting the measurement of wavelength and radiant power

Answer 22

Spectrophotometer
Spectrometer

Question 23

Branch of spectrophotometry in which the absorption measurement is made in the visible region of the spectrum

Answer 23

Colorimetry

Question 24

Instruments which have the necessary electronics to permit measurement of radiant power with a filter instead of prism or diffraction grating, to increase the sensitivity of the measurement.

Answer 24

Colorimeter Filter photometer

Question 25

The energy in the electromagnetic spectrum occurs in bundles known as

Answer 25

Photons/Quanta

Question 26

amount of energy possessed by electromagnetic radiation is expressed in

Answer 26

E = hv E = Energy v = Frequency h = Planck's constant (6.63 x10-34 joule sec) ⭐

Question 27

Energy is iversely proportional to the

Answer 27

Wavelength

Question 28

Energy is directly porportional to the

Answer 28

Wave number

Question 29

The shorter the _________ or the greater the _____________, the higher the ______

Answer 29

The shorter the wavelength or the greater the frequency, the higher the energy

Question 30

Components of spectrophotometer

Answer 30

Light sources MonochromatorSample cell Detector or transducer Device to readout response

Question 31

Light of a single wavelength

Answer 31

Monochromatic

Question 32

The mnochromatic light that passes to the sample

Answer 32

Incident light

Question 33

The monochromatic light that is not absorbed emerges from the sample

Answer 33

Transmitter light

Question 34

Beer-Lambert Formula

Answer 34

T = P/Po T = Transmittance P = Power of incident beam Po = Power of transmitted radiant beam

Question 35

The amount of the monochromatic light absorbed by the sample

Answer 35

Absorbance

Question 36

Laws of Spectrophotometry

Answer 36

Beer's Law Lambert's Law / Bourguer's Law Beer-Lambert's Law / Bourguer's Law

Question 37

The power of transmitted radiant beam decreases exponentially as concentration of the solution increase arithmetically

Answer 37

Beer's Law

Question 38

Beer's Law Equation

Answer 38

A = abc A = Absorbance a = Absorptivity b = Cell thickness c = Concn

Question 39

The power of a transmitted radiant beam decreases exponentially as the thickness of the solution increases arithmetically

Answer 39

Lambert's / Bouguer's Law

Question 40

The power of the incident and transmitted radiant beams to the thickness and concentartion of the solution

Answer 40

Beer-Lambert's Law / Bourguer's Law