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Analytical Chem 2 > Spectrophotometry > Flashcards

Spectrophotometry Flashcards

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1
Q

intensity of radiant energy transmitted, reflected or emitted is related to concentration of chemical species that absorbs energy

A

Spectrophotometry

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2
Q

Measurements of Spectrometry

A

Measurement of the position/wavelength in the electromagnetic spectrum where radiant energy has interacted with chemical species.
Measurement of the power of transmitter, emitted, reflected, or fluoresced energy.

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3
Q

The complete system if energy propagated in wave form

A

Electromagnetic Spectrum

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4
Q

Form of energy that is transmitted through space and energies; energy in wave form

A

Radiant Energy

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5
Q

Radiant Energy

A

Electromagnetic Radiant

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6
Q

Radiant Energy is energy in

A

UV Region
Visibile Region
Infrared Region

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7
Q

Length of a complete wave or cycle, from the peak of one wave to the peak of the next

A

Wavelength

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7
Q

Length of a complete wave or cycle, from the peak of one wave to the peak of the next

A

Wavelength

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8
Q

Wavelength Range of Ultraviolet Region

A

200 - 380 nm ⭐

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9
Q

Wavelength Range of Visible Region

A

380 - 780 nm

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10
Q

Wavelength Range of Near Infrared Region

A

780 - 3000 nm

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10
Q

Wavelength Range of Near Infrared Region

A

780 - 3000nm

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11
Q

Wavelength Range of medium infrared Region

A

3 -15mm ⭐

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12
Q

Wavelength Range of Far infrared Region

A

15 - 300mm

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13
Q

Units of wavelength measurement ⭐

A

Nanometer
Micrometer
Angstrong

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14
Q

Number of waves per centimeter and is equal to 1/wavelength (cm)

A

Wave number

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15
Q

Number of complete cycles which pass a given point per second

A

Frequency

16
Q

A molecule containing chromophore

A

Chromogen

17
Q

A functional group which absorb radiant energy in the UV or visible regions of the spectrum

A

Chomophore

18
Q

Examples of Chromophore

A

Organic Acids
Aldehydes
Ketone
Acetylene
Ethylene
Azomethines

19
Q

Enhance absorption by a chromophore and shift the wavelength of absorption, when attached to the chromophore

A

Auxochrome

20
Q

Possess one or more unshared or lone pair of electron that interacts with other e- in chromophore

A

Auxochrome

21
Q

Branch of spectrometery whhch embraces the measurement of the absorption

A

Spectrophotometry

22
Q

Designate instruments which have a radiant energy-dispersing device, such as prism or grating, permiting the measurement of wavelength and radiant power

A

Spectrophotometer
Spectrometer

23
Q

Branch of spectrophotometry in which the absorption measurement is made in the visible region of the spectrum

A

Colorimetry

24
Q

Instruments which have the necessary electronics to permit measurement of radiant power with a filter instead of prism or diffraction grating, to increase the sensitivity of the measurement.

A

Colorimeter
Filter photometer

25
Q

The energy in the electromagnetic spectrum occurs in bundles known as

A

Photons/Quanta

26
Q

amount of energy possessed by electromagnetic radiation is expressed in

A

E = hv
E = Energy
v = Frequency
h = Planck’s constant (6.63 x10-34 joule sec) ⭐

27
Q

Energy is iversely proportional to the

A

Wavelength

28
Q

Energy is directly porportional to the

A

Wave number

29
Q

The shorter the _________ or the greater the _____________, the higher the ______

A

The shorter the wavelength or the greater the frequency, the higher the energy

30
Q

Components of spectrophotometer

A

Light sources
MonochromatorSample cell
Detector or transducer
Device to readout response

31
Q

Light of a single wavelength

A

Monochromatic

32
Q

The mnochromatic light that passes to the sample

A

Incident light

33
Q

The monochromatic light that is not absorbed emerges from the sample

A

Transmitter light

34
Q

Beer-Lambert Formula

A

T = P/Po

T = Transmittance
P = Power of incident beam
Po = Power of transmitted radiant beam

35
Q

The amount of the monochromatic light absorbed by the sample

A

Absorbance

36
Q

Laws of Spectrophotometry

A

Beer’s Law
Lambert’s Law / Bourguer’s Law
Beer-Lambert’s Law / Bourguer’s Law

37
Q

The power of transmitted radiant beam decreases exponentially as concentration of the solution increase arithmetically

A

Beer’s Law

38
Q

Beer’s Law Equation

A

A = abc

A = Absorbance
a = Absorptivity
b = Cell thickness
c = Concn

39
Q

The power of a transmitted radiant beam decreases exponentially as the thickness of the solution increases arithmetically

A

Lambert’s / Bouguer’s Law

40
Q

The power of the incident and transmitted radiant beams to the thickness and concentartion of the solution

A

Beer-Lambert’s Law / Bourguer’s Law

Analytical Chem 2 (17 decks)

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