single stranded DNA phage plus single-stranded RNA phage Flashcards

1
Q

a + single strand DNA phage ?

A

Phage ΦX174(same strand as mRNA)

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2
Q

To perfom dsDNA,replicative form we need?

A

synthezise the minus strand

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3
Q

first step of replication by rolling circle ?

A

Replication form DNA is nicked by gene A protein

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4
Q

second step of rolling circle?

A

new + strand begins synthesis

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5
Q

third step of rolling circle ?

A

continued extension or original + strand with synthesis of new strand

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6
Q

fourth step of rolling circle?

A

one revolution complete and one progeny virus made

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7
Q

What the results of the rolling circle?

A
    • strand

- -/+ strand

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8
Q

what can we do with the + strand?

A

refaire le meme cycle

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9
Q

Single-stranded RNa phage who is one?

A

phage MS2

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10
Q

phage MS2 is what?

A

plus strand RNA phage

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11
Q

in single-stranded RNA phage the genome is used first?

A

as an mRNA(translation direct)

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12
Q

the genome used as first mRNA?

A

directs the synthesis of an RNA replicase(RNA-dependent RNA polymerase) and other viral proteins

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13
Q

The synthesis of RNA replicase is not performed by?

A

The host (on ne passe pas de - a + strand)

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14
Q

the RNA replicase synthesizes?

A

a minus strand RNA t

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15
Q

the minus strand RNA is used to produce?

A
  • additional mRNA

- additional copies of the genome (+ strand) for the production of new virion

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16
Q

la translation direct donne quoi?

A

viral proteins