single stranded DNA phage plus single-stranded RNA phage Flashcards
a + single strand DNA phage ?
Phage ΦX174(same strand as mRNA)
To perfom dsDNA,replicative form we need?
synthezise the minus strand
first step of replication by rolling circle ?
Replication form DNA is nicked by gene A protein
second step of rolling circle?
new + strand begins synthesis
third step of rolling circle ?
continued extension or original + strand with synthesis of new strand
fourth step of rolling circle?
one revolution complete and one progeny virus made
What the results of the rolling circle?
- strand
- -/+ strand
what can we do with the + strand?
refaire le meme cycle
Single-stranded RNa phage who is one?
phage MS2
phage MS2 is what?
plus strand RNA phage
in single-stranded RNA phage the genome is used first?
as an mRNA(translation direct)
the genome used as first mRNA?
directs the synthesis of an RNA replicase(RNA-dependent RNA polymerase) and other viral proteins
The synthesis of RNA replicase is not performed by?
The host (on ne passe pas de - a + strand)
the RNA replicase synthesizes?
a minus strand RNA t
the minus strand RNA is used to produce?
- additional mRNA
- additional copies of the genome (+ strand) for the production of new virion