Identification Flashcards
how does the identification of an unknown microorganism works?
depend on the comparison of its properties with those of organisms that have already been classified and named (Type strains)
type of things that u can compare with?
- Morphology
- Biochemical properties
- 16s rRNA gene sequencing
- MLST
techniques?
- Dichotomous key(morpholy and phenotype)
- Serotyping
what do they also use??
use of selective and differential methods
for exemple?
-glucose fermentation
From left to right: +,+ (GAS present),-,
-API strips:
API strips?
sets of
differential methods
and biochemical
tests
serotyping?
based on the binding of specific antibody to surface structure
for exemple who binds to LPS ?
o serotypes
Who binds to capsules?
K serotypes
Who binds to flagella?
H serotypes
Antibodies
molecules of the immune system that recognize and bind to molecule on the surface of a microorganism or to secreted proteins.
if what ur looking for is in the sample there is going to be a positive reaction?
which is agglutination
serotype c quoi ds le fond?
refer to separate groups within a species of a microorganisms that all share a similar characteristic.they all have the same antigen
pour trouver leur serotype(antigen) tu dois?
use antibodies to form a positive reaction to find what is the antigen on the cells