Physical methods

Question 1

Heat is the most widely used?

Answer 1

physical method for controlling microbial growth

Question 2

high temperature?

Answer 2

denature macromolecules

Question 3

amount of time required to reduce viability tenfold is called

Answer 3

the decimal reduction time (D)

Question 4

The decimal reduction time is

inversely correlated with

Answer 4

the

temperature

Question 5

The time necessary to kill a
defined fraction is ____ of
the initial cell concentration.

Answer 5

independant

Question 6

thermophiles?

Answer 6

ils ont des T plus haute pour arriver a leur D

Question 7

Thermal death time?

Answer 7

time needed to kill all cells at a given temperature

Question 8

Thermal death time depends on ?

Answer 8

dependent on the population size

Question 9

why?

Answer 9

Need to standardize the starting number of cells

to be able to compare the sensitivity of different microorganisms

Question 10

Endospores survive heat that would rapidly kill _____ cells

Answer 10

vegetative cells

Question 11

A _____

temperature is needed to kill endospores

Answer 11

higher

Question 12

Endospores can resist boiling

for a long time: ____ for a decimal reduction

Answer 12

5 min

Question 13

What’s the autoclave?

Answer 13

sealed device that use steam under pressure

Question 14

Allows temperature of water to get above ___

Answer 14

100 C

Question 15

At 15 psi (pound-Square inch),steam reaches _____, sterilization is achieved
in ______

Answer 15

121C

-10-15 min

Question 16

what kills the microorganisms?

Answer 16

High temperature not microorganism

Question 17

in an autoclave, The temperature of the ___ rises and falls more slowly than the temperature of the ______

Answer 17

• object

- autoclave

Question 18

The temperature of the object must reach the target temperature and be held for_____min to ensure sterility

Answer 18

10-15 min