SG 4.4 Flashcards
function of mRNA
contains message and codes for amino acid sequence
function of tRNA
transfers amino acids and contains anticodons that pair with mRNA codons in a correct order
function of rRNA
facilitates matching of mRNA and tRNA and joins amino acids to synthesize proteins
A site
matches correct incoming tRNA to the next codon in the mRNA
P site
holds tRNA carrying growing polypeptide chain
E site
empty tRNA leaves ribosome from exit site
gene expression
the process by which a gene gets turned on in a cell to make RNA and proteins
What 2 components were required for successful protein production?
At Least one positive transcription factor and an RNA polymerase
What 2 components stopped/slowed successful protein production?
Having no transcription factor or adding a negative transcription factor.
What 3 factors/components increased the rate of transcription?
a high concentration of positive TF, a high affinity of the Positive TF to the DNA, and a high affinity of RNA polymerase to the DNA and TF
What 2 factors/components stopped/slowed the rate of transcription?
The High Concentration of negative TF and the high affinity of negative TF to the DNA.
What 3 factors/components increased GFP production?
Positive TF Concentration, Positive TF Affinity, and Polymerase Affinity
What 2 factors/components stopped/decreased GFP production?
mRNA Destroyer Concentration and Protein Degradation.
mutation
change in DNA
causes of mutation
DNA replication, mutagens, and viral infections
