Serology & Heartworm QUIZ

Question 1

Answer 1

A+

Question 2

a. Test is more specific, than sensitive
b. Test is more sensitive than specific
c. Test is equally sensitive and specific
d. It can’t be determined from this data

Answer 2

b. Test is more sensitive than specific

Question 3

a. 1:1,000
b. 1:10
c. 1:10,000
d. It can’t be determined with this information

Answer 3

a. 1:1,000

Question 4

Measuring levels of antibodies against Disease X in the serum of a patient is
1. Indirect serology
2. Direct serology

Answer 4

1. Indirect serology

Question 5

Wash steps are very important in antigen-antibody complex testing to prevent…
1. False positives
2. False negatives

Answer 5

1. False positives

Question 6

When evaluating an animal for active infection of a specific disease, the minimum serology requirements are…
1. The convalescent titer is 4x higher than the acute titer
2. The convalescent titer is 2x higher than the acute titer
3. The acute titer is 4x higher than the convalescent titer
4. The acute titer is 2x higher than the convalescent titer

Answer 6

1. The convalescent titer is 4x higher than the acute titer

Question 7

1. undetermined, the test failed its controls
2. patient is positive for both FeLV and FIV
3. patient is positive for FeLV
4. patient is positive for FIV

Answer 7

4. patient is positive for FIV

Question 8

a. 1:160
b. 1:320
c. 160
d. t can not be determined from this diagram

Answer 8

a. 1:160

Question 9

What test is the test of choice for diagnosing Heartworm Disease in cats?

Answer 9

Antibody test

Question 10

If you are asked to dilute a cleaning solution 1:10 and the final volume will be 1 liter, how much concentrate (C) and how much water (W) will you mix together?
a. 900 ml C and 100 ml W
b. 100 ml C and 1000 ml W
c. it can’t be determined from this information
d. 100ml C and 900 ml W

Answer 10

d. 100ml C and 900 ml W

Question 11

An example of a false negative is:
1. a diseased animal incorrectly identified as healthy
2. a healthy animal is correctly identified as healthy
3. a diseased animal correctly diagnosed as diseased
4. a healthy animal incorrectly identified as diseased

Answer 11

1. a diseased animal incorrectly identified as healthy

Question 12

An example of a microfilarial test for Heartworm diagnosis is:
1. Modified Knott’s Test
2. Filter Technique
3. Direct Smear
4. all choices are correct

Answer 12

4. all choices are correct

Question 13

List two limitations of antigen testing (dog or cat) for diagnosis of Heartworm. (i.e. when the test won’t be accurate)
NOT GRADED YET

Answer 13

1. low female (or none) count in the blood
2. if the patient is on heartworm preventatives it may interfere with results

Question 14

Describe what is happening during an ELISA test. (e.g. describe the technology of how it works, not how the tech adds drops to a cartridge)
NOT GRADED YET

Answer 14

sample is absorbed into the matrix where it is introduced to the capture reagents for the first time. Once the test is snapped, amplifying and wash solution are released. This helps improve the specificity of the test by clearing debris. The sample hits the absorbent block which makes the flow go back the other direction, so the sample will pass through the reagents for a second time.