Advanced Hematology QUIZ Flashcards
In healthy bovine blood, the largest % of leukocytes will be
Lymphocytes
A leukocyte that presents antigen to the Specific Immune System (Humoral System)
1. lymphocyte
2. neutrophil
3. monocyte/macrophage
4. eosinophil
- monocyte/macrophage
A special stain to identify reticulocytes is
1. Giemsa stain
2. Wright’s stain
3. New Methylene blue
4. Sudan III
- New Methylene blue
The percentage of whole blood that is composed of erythrocytes is measured by
1. MCH
2. PCV
3. MCHC
4. MCV
- PCV
A Band Cell is
1. a musical cell
2. an immature granulocyte
3. a cancer cell
4. contains lines due to stain artifact
- an immature granulocyte
When evaluating a blood smear, the feathered edge is a good location to evaluate
1. microfilaria
2. large atypical cells
3. all choices are correct
4. platelet clumps
- all choices are correct
Which test differentiates rouleax formation from agglutination?
1. Activated Clotting Time
2. New Methylene Blue Test
3. Cross-match
4. Saline Test
- Saline Test
Band Cells are characterized by
1. size compared to RBCs
2. presence of blue granules
3. Dohle bodies
4. nucleus constriction is < 1/3 of widest part
- nucleus constriction is < 1/3 of widest part
Echinocytes are caused by
1. Lead toxicity
2. Neoplasia
3. EDTA artifact
4. Autoimmune Hemolytic Anemia (AIHA)
- EDTA artifact
Rouleaux formation is normal in which species?
1. can be normal in all of the species listed
2. cow and cat
3. cat and horse
4. dog and horse
- cat and horse
Please identify this equine white blood cell.
1. Reactive lymphocyte
2. Blast cell
3. Toxic band
4. Monocyte
- Monocyte
Because:
Size - 3 RBCs across
Nucleus- horse-shoe shaped
Cytoplasm contains vacuoles - common in this phagocytic cell when it is active
(too large to be toxic band; wrong nucleus to be reactive lymphocyte or blast cell)
The presence of toxic neutrophils indicate
1. a stimulus that shortens maturation in the bone marrow.
2. that the animal has been poisoned.
3. that the animal must have a viral infection.
4. that the neutrophils were damaged during smear/staining techniques.
- a stimulus that shortens maturation in the bone marrow.
- platelet clumping
- fibrin clumping
- stain precipitate
- blood parasites
- platelet clumping
- skip them because they are not blood cells.
- record these as cancer cells.
- record these as large lymphocytes.
- have slide reviewed by a DVM.
- have slide reviewed by a DVM.
Why?
These very large lymphocytes could be either large reactive OR cancer. A specialist would need to identify (special stains might be needed). Describe what you see in the report, but be certain to ask for a DVM/Pathologist to review!
- hypochomasia
- polychromasia
- normal
- immature
- hypochomasia
- PMN
- mast cell
- toxic band cell
- monocyte
- toxic band cell
Why?
This immature neutrophil (simple U nucleus) is about the same size as the mature neutrophil in the image (therefore not a monocyte). It shows the open chromatin and large clear granules of and RNA (purple dots) typical of toxic change.
“A” shows flame tipped shape/feathered edge and covers at least 2/3 the length of slide.
“D” is also pretty good…shows a little streaking at flame tip.
